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Wheat pp 435-447 | Cite as

Culture of Isolated Pollen of Wheat (Triticum aestivum L.)

  • S. K. Datta
  • I. Potrykus
  • M. Bolik
  • G. Wenzel
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 13)

Abstract

Pollen culture provides a system with haploid single cells and thus is a promising haplopahase tool for basic and applied biotechnological breeding programs. Anther culture (Nitzsche and Wenzel 1977; de Buyser and Henry 1980, 1986; Maheshwari et al. 1980; Bajaj 1983; Schaeffer et al. 1984; Wenzel and ForoughiWehr 1984; Bajaj and Gosal 1986; Dunwell 1986; Han 1986; Keller et al. 1987) is one of the methods of haploid induction most commonly used now for crop improvement. Culture of whole spikes (Wilson 1977; Brettel et al. 1980, Ozias-Akins and Vasil 1982; Datta 1987) may simplify this technique. The third way is the culture of isolated microspores. However, in most reports the microspores are cultured within the anthers. Only a rather limited number of reports have been published on the successful use of isolated microspore culture in cereals (Köhler and Wenzel 1985; Wei et al. 1986; Datta and Wenzel 1987, 1988; Datta and Potrykus 1988). While regeneration of plants from single cells of cereals is still a problem, plant regeneration from in vitro cultures originating from multicellular explants has been achieved from all the major cereal crops (Vasil 1983; Lörz et al. 1988). Plant regeneration from microspores of wheat follows the two general pathways of in vitro culture, viz organogenesis and embryogenesis. The latter one is preferred for its unicellular origin and production of non-chimeric plants.

Keywords

Somatic Embryogenesis Plant Regeneration Anther Culture Isolate Microspore Culture Pollen Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • S. K. Datta
    • 1
  • I. Potrykus
    • 1
  • M. Bolik
    • 2
  • G. Wenzel
    • 2
  1. 1.Swiss Federal Institute of TechnologyETH, Institute of Plant SciencesZürichSwitzerland
  2. 2.Institute for Resistance GeneticsBBAGrünbach-BockhornGermany

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