Wheat pp 285-352 | Cite as

Wheat Anther Culture: Agronomic Performance of Doubled Haploid Lines and the Release of a New Variety “Florin”

  • Y. Henry
  • J. de Buyser
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 13)


The production of haploid plants from hybrids, followed by chromosome doubling, provides wheat breeders with a means of accelerating the process of true breeding line development. The interest of doubled haploid wheat plants has been understood since their discovery (Gaines and Aase 1926). Nevertheless, a useful technique for producing haploids started at the beginning of the 1970’s with anther culture (Chu et al. 1973; Ouyang et al. 1973; Picard and de Buyser 1973; Wang et al. 1973). Since that time, other techniques have been developed in order to produce haploid wheat plants: the bulbosum technique used for some particular genotypes (Barclay 1975), the Salmon method (Kobayashi and Tsunewaki 1978; Tsunewaki et al. 1984) and the wheat × maize crosses (Laurie and Bennet 1988). Limited numbers of haploids can also be produced using irradiated pollen (Natarajan and Swaminathan 1958; Snape et al. 1983) and unpollinated ovary culture (Zhu and Wu 1979; Yan et al. 1979).


Double Haploid Anther Culture Regeneration Ability Haploid Plant Albino Plant 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • Y. Henry
    • 1
  • J. de Buyser
    • 1
  1. 1.C.N.R.S., Laboratoire de Génétique Végétale, URA 115Université Paris XIOrsay, Cedex 05France

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