Wheat pp 250-261 | Cite as

Embryo Culture of Wheat — Regenerative Tissue Culture System

  • T. Shimada
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 13)

Abstract

Embryo culture has often been applied to practical problems in plant breeding. For example, inviable embryos in vivo, resulting from unsuccessful crosses, can be grown in vitro in a nutrient medium. This topic has been frequently discussed (see Raghavan 1977; Norstog 1979). Young embryos of cereals can be cultured in vitro to obtain regenerative cultured tissues from which many roots and shoots can be initiated. Regenerative cultures have been obtained by utilizing immature embryos in maize (Green and Phillips 1975), oats (Cummings et al. 1976), sorghum (Gamborg et al. 1977; Thomas et al. 1977), barley (Dale and Deambrogio 1979), triticale (Sharma et al. 1980), and wheat (Shimada 1978; Gosch-Wackerle et al. 1979).

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Adachi T, Katayama Y (1989) Callus formation and shoot differentiation in wheat tissue culture. Bull Fac Agric Univ Miyazaki 16: 77–82Google Scholar
  2. Ahloowalia BS (1982) Plant regeneration from callus culture in wheat. Crop Sci 22: 405–410CrossRefGoogle Scholar
  3. Bajaj YPS (1986) In vitro regeneration of diverse plants and the cryopreservation ofgermplasm of wheat (Triticum aestivum L.) Cereal Res Commun 14: 305–311Google Scholar
  4. Cummings DP, Green CE, Stuthman DD (1976) Callus induction and plant regeneration in oats. Crop Sci 16: 465–470CrossRefGoogle Scholar
  5. Dale PJ, Deambrogio E (1979) A comparison of callus induction and plant regeneration from different explants of Hordeum vulgare. Z Pflanzenphysiol 94: 65–77Google Scholar
  6. Dudits D, Nemet G, Haydu Z (1975) Study of callus growth and organ formation in wheat (Triticum aestivum) tissue cultures. Can J Bot 53: 957–963CrossRefGoogle Scholar
  7. Dunstan DI, Short KC, Thomas E (1978) The anatomy of secondary morphogenesis in cultured scutellum tissues of Sorghum bicolor. Protoplasma 97: 251–260CrossRefGoogle Scholar
  8. Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151–158PubMedCrossRefGoogle Scholar
  9. Gamborg OL, Shyluck JP, Brar DS, Constabel F (1977) Morphogenesis and plant regeneration from callus of immature embryos of sorghum. Plant Sci Lett 10: 67–74CrossRefGoogle Scholar
  10. Gosch-Wackerle G, Avivi GL, Galun E (1979) Induction, culture and differentiation of callus from immature rachises, seeds and embryos of Triticum. Z Pflanzenphysiol 91: 267–278Google Scholar
  11. Green CE, Phillips RL (1975) Plant regeneration from tissue culture of maize. Crop Sci 15: 417–421CrossRefGoogle Scholar
  12. Heyser JW, Nabors MW, McKinnon C, Dykes TA, Demott KJ, Kautzman DC, Mujeeb-Kazi A (1985) Long-term, high-frequency plant regeneration and the induction of somatic embryogenesis in callus cultures of wheat (Triticum aestivum L.). Z Pflanzenzücht 94: 218–233Google Scholar
  13. Karp A, Maddock SE (1984) Chromosome variation in wheat plants regenerated from cultured immature embryos. Theor Appl Genet 67: 249–255CrossRefGoogle Scholar
  14. Lapitan NLV, Sears RG, Gill BS (1984) Translocations and other karyotypic structural changes in wheat X rye hybrids regenerated from tissue culture. Theor Appl Genet 68: 547–554CrossRefGoogle Scholar
  15. Larkin PJ, Scowcroft WR (1981) Somaclonal variation — a novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60: 197–214CrossRefGoogle Scholar
  16. Larkin PJ, Ryan SA, Brettell RJS, Scowcroft WR (1984) Heritable somaclonal variation in wheat. Theor Appl Genet 67: 443–455CrossRefGoogle Scholar
  17. Lazar MD, Collins CB, Vian WE (1983) Genetic and environmental effects on the growth and differentiation of wheat somatic cell cultures. J Hered 74: 353–357Google Scholar
  18. Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 18: 100–127CrossRefGoogle Scholar
  19. Lu C, Vasil IK, Ozias-Akins P (1982) Somatic embryogenesis in Zea mays L. Theor Appl Genet 62: 109–112CrossRefGoogle Scholar
  20. Maddock SE, Lancaster VA, Risiott R, Franklin J (1983) Plant regeneration from cultured immature embryos and inflorescences in 25 cultivars of wheat (Triticum aestivum). J Exp Bot 34: 915–926CrossRefGoogle Scholar
  21. Magnusson I, Bornman CH (1985) Anatomical observations on somatic embryogenesis from scutellar tissues of immature zygotic embryos of Triticum aestivum. Physiol Plant 63: 137–146CrossRefGoogle Scholar
  22. Maekawa KM (1981) Genetic studies on alloplasmic lines of three hexaploid synthetics in Triticinae. MS Thesis, Fac Agric Kyoto UnivGoogle Scholar
  23. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant 15: 473–497CrossRefGoogle Scholar
  24. Nakamura T, Keller WA (1982) Callus proliferation and plant regeneration from immature embryos of hexaploid triticale. Z Pflanzenzücht 88: 137–160Google Scholar
  25. Norstog K (1979) Embryo culture as a tool in the study of comparative and developmental morphology. In: Sharp WR, Larsen PO, Paddock EF, Raghavan V (eds) Plant cell and tissue culture: principles and applications. Ohio State Univ Press, Columbus, pp 179–202Google Scholar
  26. Ogura H, Shimada T (1978) Preliminary report on plant redifferentiation from wheat callus. Wheat Inf Sery 45,46: 26–28Google Scholar
  27. O’Hara JF, Street HE (1978) Wheat callus culture: the initiation, growth and organogenesis of callus derived from various explant sources. Ann Bot (London) 42: 1029–1038Google Scholar
  28. Ozias-Akins P, Vasil IK (1982) Plant regeneration from cultured immature embryos and inflorescences of Triticum aestivum L. (wheat); evidence for somatic embryogenesis. Protoplasma 110: 95–105CrossRefGoogle Scholar
  29. Ozias-Akins P, Vasil IK (1983) Improved efficiency and normalization of somatic embryogenesis in Triticum aestivum (wheat). Protoplasma 117: 40–44CrossRefGoogle Scholar
  30. Raghavan V (1977) Applied aspects of embryo culture. In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspect of plant cell, tissue, and organ culture. Springer, Berlin Heidelberg New York, pp 375–397Google Scholar
  31. Sears RG, Deckard EL (1982) Tissue culture variability in wheat: callus induction and plant regeneration. Crop Sci 22: 546–550CrossRefGoogle Scholar
  32. Sharma GC, Belb LL, Sapra VT (1980) Genotypic differences in organogenesis from callus often triticale lines. Euphytica 29: 751–754CrossRefGoogle Scholar
  33. Shimada T (1978) Plant regeneration from the callus induced from wheat embryos. Jpn J Genet 53: 371–374CrossRefGoogle Scholar
  34. Shimada T, Yamada Y (1979) Wheat plants regenerated from embryo cell cultures. Jpn J Genet 54: 379–385CrossRefGoogle Scholar
  35. Shimada T, Sasakuma T, Tsunewaki K (1969) In vitro culture of wheat callus tissue. I. Callus formation, organ redifferentiation and single cell culture. Can J Genet Cytol 11: 294–304Google Scholar
  36. Shimada T, Koba K, Otani M, Niizeki H (1987) Morphology, meiosis and in vitro propagation of barley-wheat hybrids. In: Yasuda S, Konishi T (eds) Barley genetics V. 5th Int Barley Genetics Symp, OkayamaGoogle Scholar
  37. Springer WD, Green CE (1979) A cytological examination of tissue culture initiation from immature embryos of maize. Protoplasma 101: 269–281CrossRefGoogle Scholar
  38. Thomas E, King P, Potrykus I (1977) Shoot and embryo-like structure formation from cultured tissues of Sorghum bicolor. Naturwissenshaften 64: 587CrossRefGoogle Scholar
  39. Trione EJ, Jones LE, and Metzger WI (1968) In vitro culture of somatic wheat callus tissue. Am J B of 55: 529–531CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • T. Shimada
    • 1
  1. 1.Research Institute of Agricultural ResourcesIshikawa Agricultural CollegeIshikawaJapan 921

Personalised recommendations