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Leghemoglobins and Their Gene Expressions

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The Nitrogen Fixation and its Research in China

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Abstract

All nitrogen-fixing legume root nodules resulting from infection by symbiotic Rhizobium contain leghemoglobin. Leghemoglobin facilitates and regulates the flow of oxygen to the nitrogen-fixing bacteroids, resulting in creating the microaerobic environment necessary for the nitrogenase activity (Wittenberg et al. 1972; 1974; Wittenberg 1976). Leghemoglobins from soybean (Appleby et al. 1975; Ellfolk 1960), kidney bean (Lehtovaara et al. 1974), yellow lupine (Broughton et al. 1972; Kuranova et al. 1976), serradella (Broughton et al. 1972), broad bean (Richadson et al. 1975), snake bean (Broughton et al. 1971), Seshania (Bogusz et al. 1987; Gu et al. 1989), which form large round nodules, and from pea (Lehtovaara et al. 1980), clover (Thulborn et al. 1979), alfalfa (Jing et al. 1982, 1983a; Lullien et al. 1987), which form elongated nodules with terminal meristems, have been purified and studied.

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References

  • Appleby CA, Nicola NA, Hurrell JG, Leach Si (1975) Characterization and improved separation of soybean leghemoglobin. Biochem 14: 4444

    Article  CAS  Google Scholar 

  • Appleby CA, Tjepkema JD, Trinick MJ (1983) Hemoglobin in a nonleguminous plant, parasponia: possible genetic origin and function in nitrogen fixation. Science 220: 951

    Article  CAS  Google Scholar 

  • Appleby CA (1984) Leghemoglobin and Rhizobium respiration. Ann Rev Plant Physiol. 35: 443

    Article  CAS  Google Scholar 

  • Barker DG, Gallusci P, Lullien V, Khan H, Gherard M, Huguet T (1988) Identification of two groups of leghemoglobin genes in alfalfa (Medicago satira) and a study of their expression during root nodule development. Plant Mol Biol 11: 761

    Article  CAS  Google Scholar 

  • Becana M, Gogorcena Y, Aparico-Tejo BM, Sanchez-Diag M (1986) Nitrogen Fixation and leghemoglobin content during vegetative growth of alfalfa. J Plant Physiol 123: 117

    Article  CAS  Google Scholar 

  • Bogusz D, Kortt AA, Appleby CA (1987) Sesbania rostrata root and stem nodule leghemoglobin: Purification and relationship among the seven major components. Arch Biochem Biophys 254: 261

    Google Scholar 

  • Bogusz D, Appleby CA, Landsmann J, Dennis ES, Trinick MJ, Peacock WJ (1988) Functioning hemoglobin genes in non-nodulating plants. Nature 331: 178

    Article  CAS  Google Scholar 

  • Bojsen K, Abiladsten D, Jensen EO, Paluden K, Marcker KA (1983) The chromosomal arrangement of six soybean leghemoglobin genes. EM BO J 2: 1165

    CAS  Google Scholar 

  • Brison N, Verma DPS (1982) Soybean leghemoglobin gene family: Normal, pseudo, and truncated genes. Proc Natl Acad Sci USA 79: 4055

    Google Scholar 

  • Broughton WJ, Dilworth MJ (1971) Control of leghemoglobin synthesis in snake bean. Biochem J 125: 1075

    CAS  Google Scholar 

  • Broughton WJ, Dilworth MJ, Godfrey CA (1972) Molecular properties of lupine and serradella leghemoglobin. Biochem J 127: 309

    CAS  Google Scholar 

  • de Bruijn FJ, Jensen EO, Metz BA, Hoffmann HJ, Welters P, Marcker KA, Schell J (1988) Interaction of transacting factors with distinct DNA elements in the promoter region of the Glycine max and Seshania rostrata leghemoglobin genes. In: Palacios R, Verma DPS (eds) Molecular genetics of plant-microbe interaction. APS Press, St. Paul. MN USA, pp 333

    Google Scholar 

  • de Bruijn FJ, Grunenberg GFB, Hoffmann HJ, Metz B, Ratet P, Simons-Schreier A, Szabados L, Welters P, Schell J (1989) Regulation of plant genes specifically induced in nitrogen-fixing nodules: role of cis-acting elements and trans-acting factors in leghemoglobin gene expression. Plant Mol Biol 13: 319

    Article  Google Scholar 

  • Dilworth MJ, Covertry DR (1976) Stability of leghemoglobin in yellow lupine nodules. In: Newton WE, Postgate JR, Rodriguez-Barrueco C (eds) Recent developments in nitrogen fixation. Academic Press, London

    Google Scholar 

  • Ellfolk N (1960) Crystalline leghemoglobin, I. Purification procedure. Acta Chem Scand 14: 609

    Article  CAS  Google Scholar 

  • Govers F, Gloudemans T, Moerman M, van Kammen A, Bisseling T (1985) Expression of plant genes during the development of pea root nodules. EMBO J 4: 861

    Google Scholar 

  • Gu XJ, Li GS, Jing Y (1989) Leghemoglobin from root nodules of Sesbania cannabina. Chinese J Bot 1: 116

    Google Scholar 

  • Hamilton B, Hofbauer R, Ruis H (1982) Translational control of catalase synthesis by hemin in the yeast Saccharomyces cerevisiae. Proc Natl Acad Sci USA 79: 7609

    Article  CAS  Google Scholar 

  • Hattori J, Johnson DA (1985) The detection of leghemoglobin-line sequences in legumes and nonlegumes. Plant Mol Biol 4: 285

    Article  CAS  Google Scholar 

  • Holein D, McPherson J, Goh SH, Bornstein P (1981) Regulation of protein synthesis: Translational control by procollagen-drived fragments. Proc Natl Acad Sci USA 78: 6163

    Google Scholar 

  • Hurrell JGR, Broughton WJ, Dilworth HJ, Minasian E, Leach SJ (1976) Comparative structural and immunochemical properties of leghomoglobins. Eur J Biochem 66: 389

    Google Scholar 

  • Hurrell JGR, Thulborn AR, Broughton WJ, Dilworth MJ, Leach SJ (1977) Leghemoglobin immunochemistry and phylogenetic relationship. FEBS Lett 84: 244

    Article  CAS  Google Scholar 

  • Hyldig-Nielsen JJ, Jensen EO, Paludan K, Wiborg O, Garett R, Jorgensen P, Marcker KA (1982)

    Google Scholar 

  • The primary structure of two leghemoglobin genes from soybean. Nucl Acid Res 10: 689

    Google Scholar 

  • Jensen EO, Marcker KA, Villadsen IS (1986) Herne regulates the expression in Saccharomyces cerevisiae of chimeric genes containing 5’-flanking soybean leghemoglobin sequences. EMBO J 5: 843

    Google Scholar 

  • Jensen EO, Marcker KA, Schell J, de Bruijn FJ (1988) Interaction of a nodule specific, trans-acting factor with distinct DNA elements in the soybean leghemoglobin lbc3 5’-upstream region. EMBO J 7: 1265

    CAS  Google Scholar 

  • Jing Y, Paau AS, Brill WJ (1982) Leghemoglobins from alfalfa (Medicago satina L. vernal) root nodules. I. Purification and in vitro synthesis of five leghemoglobin components. Plant Sci Lett 25: 119

    Google Scholar 

  • Jing Y, Paau AS, Brill WJ (1983a) Methods of isolation and some characteristics of leghemoglobins from alfalfa (Medicago sativa L. vernal) nodules. Acta Botanica Sinica 25: 220

    CAS  Google Scholar 

  • Jing Y, Paau AS, Brill WJ (1983b) mRNAs of leghemoglobins from alfalfa root nodules. Acta Botanica Sinica 25: 431

    Google Scholar 

  • Jing Y (1985) Synthesis and cloning of cDNA coding for alfalfa leghemoglobin. Acta Botanica Sinica 27: 263

    CAS  Google Scholar 

  • Jing Y (1986) Regulation of alfalfa leghemoglobin synthesis at translational level. Scientia Sinica, Series B, 29: 50

    CAS  Google Scholar 

  • Jing Y (1987) Plant hemoglobin and its gene expression. In: Wu Xiangyu, Huang Zongzhen (eds) Advances in Plant Physiol Biochem. Chinese Academic Press, Beijing, p 141

    Google Scholar 

  • Jing Y (1988a) Synthesis and cloning of cDNAs coding for alfalfa leghemoglobins. In: Swaminathan MS, Hu H, Shao QQ (eds) Genetic manipulation in crops. CASSELL TYCOOLY, pp 237

    Google Scholar 

  • Jing Y (1988b) Expression of leghemoglobin genes of alfalfa (Medicago sativa L. vernal) during root nodule development. In: Bothe H, de Bruijn FJ, Newton.WE (eds) Nitrogen fixation: Hundred years after Gustav Fischer, Stuttgart, New York, p 636

    Google Scholar 

  • Jing Y (1988e) Expression of leghemoglobin genes in senescent and dark treatment of root nodules of alfalfa (Medicago sativa L. vernal). In: Book of Second Japan-China Bilateral Symposium on Biophysics, May 16–20, Kyoto, Japan, p 325

    Google Scholar 

  • Jing Y, Cho MJ, Paay AS, Brill WJ (1989a) Molecular cloning of cDNA synthesized from a putative leghemoglobin mRNA precursor isolated from alfalfa nodules. Chinese J Bot 1: 9

    Google Scholar 

  • Jing Y, Paau AS, Brill WJ (1990a) Transcription and translation of alfalfa leghemoglobin genes during root nodule development. Science in China 33: 1078

    Google Scholar 

  • Jing Y, Shan XQ (1990b) Nitrogen fixation and leghemoglobin synthesis during rejuvenation after dark treatment of alfalfa. Chinese J Bot 2: 7

    Google Scholar 

  • Jing Y, Li GS, Shan XQ, Li JG (1990e) Rice root nodules with acetylene reduction activity. In: Gresshoff PM, Roth E and Stacey G (eds) Nitrogen fixation: achievements and objectives. Chapman and Hall, New York, London p 829

    Google Scholar 

  • Kiss G, Vegh Z, Vincze E (1987) Nucleotide sequence of a eDNA clone encoding leghemoglobin III from Medicago satira. Nucl Acid Res 15: 3620

    Article  CAS  Google Scholar 

  • Klucas RV (1974) Studies on soybean nodule senescence. Plant Physiol 54: 612

    Article  CAS  Google Scholar 

  • Kuranova IP, Grebenko AI, Konareva NV, Syromyatnikova IF, Barynin VV (1976) Separation of

    Google Scholar 

  • leghemoglobin from lupine (Lupinus luteus L.) nodules into components. Biokim. 41: 1603

    Google Scholar 

  • LaRue TA, Child JJ (1978) Leghemoglobin and nitrogenese activity in peas. In: Proc of Steenbock- Kettering Int Symp on Nitrogen Fixation. June 12–16, 1978, University of Wisconsin (Madison)

    Google Scholar 

  • Lee JS, Brown GG, Verma DPS (1983) Chromosomal arrangement of leghemoglobin genes in soybean. Nucl Acid Res 11: 5541

    Article  CAS  Google Scholar 

  • Lehtovaara P, Ellfolk N (1974) The primary structure of kidney bean leghemoglobin. FEBS Lett 43: 239

    Article  CAS  Google Scholar 

  • Lehtovaara P, Lappalainen A, Ellfolk N (1980) The amino acid sequence of pea (Pisum satirum) leghemoglobin. Biochim Biophys Acta 623: 98

    Article  CAS  Google Scholar 

  • Lullien V, Barker DG, de Lajudie P, Huguet T (1987) Plant gene expression in effective and ineffective root nodules of alfalfa (Medicago saliva). Plant Mol Biol 9: 465

    Article  Google Scholar 

  • Marcker A, Lund M, Jensen EO, Marcker KA (1984) Transcription of the soybean leghemoglobin genes during nodule development. EMBO J 3: 1691

    CAS  Google Scholar 

  • Mauro VP, Nguyen T, Kafinkes P, Verma DPS (1985) Primary structure of the soybean nodulin-23 gene and potential regulation elements in the 5’-flanking regions of nodulin and leghemoglobin genes. Nucl Acid Res 13: 239

    Article  CAS  Google Scholar 

  • Mauro VP, Verma DPS (1988) Transcriptional-activation nuclei from uninfected soybean of a set of genes involved in symbiosis with Rhizobium. Mol Plant-Microbe Interaction, 1: 46

    Article  Google Scholar 

  • Metz B, Welters P, Hoffmann HJ, Jensen EO, Schell J, de Bruijn FJ (1988) Primary structure and promoter analysis of leghemoglohin genes stem-nodulated tropical legume Sesbania rostrata: conserved coding sequences, cis-elements and trans-acting factor. Mol Gen Gent 214: 181

    CAS  Google Scholar 

  • Metzler DE (1977) Enzymes: The protein catalysts of cells. In: Biochemistry: The chemical reaction of living cells. Academic Press, New York, SanFrancisco, London, p 301

    Google Scholar 

  • Ochoa S, de Haro C (1979) Regulation of protein synthesis in eukaryotes. Ann Rev Biochem 48: 549 Revel M, Groner Y (1978) Post-transcriptional and translational controls of gene expression in eukaryotes. Ann Rev Biochem 47: 1079

    Google Scholar 

  • Richadson M, Dilworth MJ, Seawen MD (1975) The amino acid sequence of leghemoglohin 1 from root nodules of broad been (Vicia fàba L.) FEBS Lett 51: 33

    Google Scholar 

  • Roberts MP, Jafar S, Mullin BC (1985) Leghemoglobin-like sequences in the DNA of four actinorhizal plants. Plants Mol Biol 5: 333

    Article  CAS  Google Scholar 

  • Roponen J (1970) The effect of darkness on the leghemoglohin content and amino acid levels in the root nodules of pea plants. Plant Physiol 23: 452

    Article  CAS  Google Scholar 

  • Stougaard J, Marcker KA, Otten L, Schell J (1986) Nodule-specific expression of a chimeric soybean leghemoglobin gene in transgenic Lotus corniculatus. Nature 321: 669

    Article  Google Scholar 

  • Stougaard J, Abildsten D, Marcker KA (1987a) The Agrobacterium rhizogenes pRiTL-DNA segment as a gene vector system for transformation of plants. Mol Gen Genet 207: 251

    Article  CAS  Google Scholar 

  • Stugaard J, Sandal NN, Gron A, Kuhle A, Karcker KA (1987b) 5’-analysis of the soybean leghemoglohin Lbc3 gene: regulatory element required for promoter activity and organ specificity. EMBO J 6: 3565

    Google Scholar 

  • Thulborn KR, Minasian E, Leach SJ (1979) Leghemoglobin from Trifolium subterraneum purification and characterization. Biochim Biophys Acta 578: 476

    Google Scholar 

  • Tjepkema JC (1983) Hemoglobin in the nitrogen-fixing root nodules of actinorhizal plants. Can J Bot 61: 2924

    Article  CAS  Google Scholar 

  • Tjepkema JD (1984) Oxygen, hemoglobins, and energy usage in actinorhizal nodules. In: Veeger

    Google Scholar 

  • C, Newton WE (eds) Advances in nitrogen fixation research. Martinus Nijhoff, Boston, p467 Truelsen E, Gausing K, Jochimsen B, Jorgensen P, Marcker KA (1979) Cloning of soybean leghe-

    Google Scholar 

  • moglobin structural gene sequences synthesized in vitro. Nucl Acid Res 6: 3061

    Google Scholar 

  • Verma DPS, Nash DT, Schulman HM (1974) Isolation and in vitro translation of soybean leghemoglobin mRNA. Nature 251: 74

    Article  CAS  Google Scholar 

  • Verma DPS, Delauney J (1988) Root nodule symbiosis: nodulin and nodulin genes. In: Verma DPS, Goldberg RB (eds) Plant gene research: temporal and spatial regulation of plant genes. Springer-Verlag, Wien, New York, p 169

    Chapter  Google Scholar 

  • Welters P, Metz BA, Schell J, de Bruijn FJ (1989) Nucleotide sequence of the Sesbania rostrata leghemoglobin (Srglb3) gene. Nucl Acid Res 17: 1253

    Article  CAS  Google Scholar 

  • Wiborg O, Hyldig-Nielsen JJ, Jensen EO, Paludan K, Marcker KA (1982) The nucleotide sequences of two leghemoglobin genes from soybean. Nucl Acid Res 10: 3487

    Article  CAS  Google Scholar 

  • Wittenberg JB, Appleby CA, Wittenberg BA (1972) The kinetics of the reaction of leghemoglohin with oxygen and carbon monoxide. J Biol Chem 247: 527

    CAS  Google Scholar 

  • Wittenberg JB, Bergersen FJ, Appleby CA, Turner GC (1974) Facilitated oxygen diffusion. J Biol Chem 249: 4057

    CAS  Google Scholar 

  • Wittenberg JB (1976) Facilitation of oxygen diffusion by intracellular leghemoglohin and myoglohin. In: Jobsis FF (ed) Oxygen and physiological functions. Prof Inf Lib, Dallas, Texas, p 228

    Google Scholar 

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Authors
  1. Y. X. Jing
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  2. X. J. Gu
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  3. G. S. Li
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  4. X. Q. Shan
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Editors and Affiliations

  1. Shanghai Institute of Biochemistry, Academia Sinica, 320 Yue-Yang Road, 200031, Shanghai, PR China

    Guo-Fan Hong

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© 1992 Springer-Verlag Berlin Heidelberg

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Jing, Y.X., Gu, X.J., Li, G.S., Shan, X.Q. (1992). Leghemoglobins and Their Gene Expressions. In: Hong, GF. (eds) The Nitrogen Fixation and its Research in China. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-10385-2_21

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  • DOI: https://doi.org/10.1007/978-3-662-10385-2_21

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-10387-6

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