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Regeneration of Plants from Protoplasts of Coffea spp. (Coffee)

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Plant Protoplasts and Genetic Engineering VII

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 38))

Abstract

In many tropical countries, coffee is a crop of major economic importance, generating a total of 10–12 billion US dollars per year (Söndahl and Lauritis 1992). About 75% of the cultivated coffee is Coffea arabica, with the remaining 25% being C. canephora, also known as Robusta coffee. Conventional coffee breeding has led to major improvements in yield and in resistance to pests and diseases. However, there are important obstacles that impede coffee improvement. The utilization of the more than 70 different members of the genus Coffea for the introduction of desirable traits into C. arabica is complicated by genetic barriers, since this is the only species of the genus that is autogamous and tetraploid. Another problem in coffee improvement is the long time required for breeding programs, a fact inherent in all woody crop plants. The application of biotechnological methods has the potential to overcome some of these difficulties.

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References

  • Acuña JR, de Peña M (1991) Plant regeneration from protoplasts of embryogenic cell suspensions of Coffea arabica L. cv. caturra. Plant Cell Rep 10: 345–348

    Article  Google Scholar 

  • Acuña R, de Peña M (1987) Isolation and culture of coffee protoplasts. In: Angarita Zerda A (ed) Abstr Int Congr Plant Tissue Cult, Tropical Species, Bogotà 1987. Univ Nacional de Colombia, Bogotà, pp 34–35

    Google Scholar 

  • Adams TL, Zarowitz MA (1994) Stably transformed coffee plant cells and plantlets. US Patent and trademark office. Patent #5,334,529, Aug 2, 1994

    Google Scholar 

  • Barton CR, Adams TL, Zarowitz MA (1992) Stable transformation of foreign DNA into Coffea arabica plants. In: Proc 14th Int Colloq Coffee, 14–19 July 1991, San Francisco. ASIC, Paris pp 745–747

    Google Scholar 

  • Baumann TW (1986) Biotechnology, its potential for the growth and manufacture of coffee. In: Proc 11th Int. Colloq Coffee, 11–15 Feb 1985, Lome. ASIC, Paris, pp 55–68

    Google Scholar 

  • Baumann TW, Neuenschwander B (1990) Tissue culture in coffee biotechnology. Café Cacao Thé 34: 159–164

    Google Scholar 

  • Baumann TW, Rodriguez MF, Kappeler AW (1992) Chlorogenic acid in leaf disks, suspension-cultured cells, and protoplasts of coffee (Coffea arabica L.). In: Proc 14th Colloq Coffee, 14–19 July 1991, San Francisco. ASIC, Paris, pp 465–470

    Google Scholar 

  • Blaydes DF (1966) Interaction of kinetin and various inhibitors in the growth of soybean tissue. Physiol Plant 19: 748–753

    Article  CAS  Google Scholar 

  • Danilina AN (1972) Morphogenesis in tissue cultures of Daucus carota. Phytomorphology 22: 160164

    Google Scholar 

  • Dentan E (1985) The microscopic structure of the coffee bean. In: Clifford MN, Willson KC (eds) Coffee: botany, biochemistry and production of beans and beverage. Croom Helm, London, pp 284–304

    Google Scholar 

  • Dublin P (1981) Embroyogenèse somatique directe sur fragments de feuilles de caféier Arabusta.

    Google Scholar 

  • Café Cacao Thé 25: 237–242

    Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151–158

    Article  PubMed  CAS  Google Scholar 

  • Garcia E, Menéndez A (1987) Embriogénesis a partir de explantes foliares del cafeto Catimor. Café Cacao Thé 31: 15–22

    Google Scholar 

  • Grèzes J, Thomas D, Thomasset B (1994) Factors influencing protoplast isolation from Coffea arabica cells. Plant Cell Tissue Organ Cult 36: 91–97

    Article  Google Scholar 

  • Kao KN (1977) Chromosomal behaviour in somatic hybrids of soybean-Nicotiana glauca. Mol Gen Genet 150: 225–230

    Article  Google Scholar 

  • Kohlenbach HW, Geier T (1972) Embryonen aus in vitro kultivierten Antheren von Datura meteloides Dun., Datura wrightii Regel und Solanum tuberosum L Z Pflanzenphysiol 67: 161–165

    Google Scholar 

  • Menéndez-Yuffa A, Garcia E (1996) Coffea species (Coffee). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 35. Trees IV. Springer, Berlin Heidelberg New York, pp 95–119

    Google Scholar 

  • Monaco LC, Söndahl MR, Carvalho A, Crocomo OJ, Sharp WR (1977) Applications of tissue culture in the improvement of coffee. In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspects of plant cell, tissue, and organ culture. Springer, Berlin Heidelberg New York, pp 109–129

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497

    Article  CAS  Google Scholar 

  • Orozco FJ, Schieder O (1984) Isolation of mesophyll protoplasts of the genus Coffea. Turrialba 34: 534–536

    Google Scholar 

  • Pfrunder R, Wanner H, Frischknecht PM, Baumann TW (1981) An attempt to localize caffein in the cell by its washout kinetics. In: Proc 9th Int Colloq Coffee, 16–20 June 1980, London. ASIC, Paris, pp 169–175

    Google Scholar 

  • Reinert J (1959) Über die Kontrolle der Morphogenese und die Induktion von Adventivembryonen an Gewebekulturen aus Karotten. Planta 53: 318–333

    Article  Google Scholar 

  • Schöpke C (1989) In vitro–Kultur bei Kaffee: Versuche zur Isolierung und Kultivierung von Protoplasten und zur Regeneration von Pflanzen. PhD Thesis, JW Goethe Univ, Frankfurt. Maraun, Frankfurt, FRG (ISBN 3–9801650–7–8)

    Google Scholar 

  • Schöpke C, Müller LE, Kohlenbach HW (1987) Somatic embryogenesis and regeneration of plantlets in protoplast cultures from somatic embryos of coffee (Coffea canephora P. ex Fr.). Plant Cell Tissue Organ Cult 8: 243–248

    Article  Google Scholar 

  • Schöpke C, Müller LE, Kohlenbach HW (1988) Coffee protoplasts: isolation, culture and plantlet regeneration. In: Proc 12th Int Colloq Coffee, 29 June–3 July 1987, Montreux. ASIC, Paris, pp 426–432

    Google Scholar 

  • Söndahl MR (1982) Tissue culture of morphological mutants of coffee. In: Fujiwara A (ed) Plant Tissue Culture 1982, Maruzen, Tokyo, pp 417–418

    Google Scholar 

  • Söndahl MR (1989) The potential impact of biotechnology in coffee. In: Proc 13th Int Colloq coffee, 21–25 Aug 1989, Paipa. ASIC, Paris, pp 407–419

    Google Scholar 

  • Söndahl MR, Lauritis JA (1992) Coffee. In: Hammerschlag FA, Litz RE (eds) Biotechnology of perennial fruit crops. CAB, Cambridge, pp 401–420

    Google Scholar 

  • Söndahl MR, Sharp WR (1979) Research in Coffea spp. and applications of tissue culture methods. In: Sharp WR, Larsen PO, Paddock EF, Raghavan V (eds) Plant cell and tissue culture — principles and applications. Ohio State Univ Press, Columbus, pp 527–584

    Google Scholar 

  • Söndahl MR, Chapman M, Sharp WR (1980) Protoplast liberation, cell wall reconstitution, and callus proliferation in Coffea arabica L. callus tissues. Turrialba 30: 161–165

    Google Scholar 

  • Söndahl MR, Nakamura T, Medina-Filho HP, Carvalho A, Fazuoli LC, Costa WM (1984) Coffee. In: Ammirato PV, Evans DA, Sharp WR, Yamada Y (eds) Handbook of plant cell culture, vol 3, Crop species. Macmillan, New York, pp 564–590

    Google Scholar 

  • Sprial J, Pétiard V (1992) Protoplast culture and regeneration in Coffea species. In: Proc 14th Int Colloq Coffee, 14–19 July 1991, San Francisco. ASIC, Paris, pp 383–391

    Google Scholar 

  • Staristky G (1970) Embryoid formation in callus tissues of coffee. Acta Bot Neerl 19: 509–514 Sybenga J (1960) Genetics and cytology of coffee. Bibliographia Genet 19: 217–316

    Google Scholar 

  • Tahara M, Yasuda T, Uchida N, Yamaguchi T (1994) Formation of somatic embryos from protoplasts of Coffea arabica L. HortScience 29: 172–174

    Google Scholar 

  • Townsley PM (1974) Production of coffee from plant cell suspension cultures. Can Inst Food Sci Technol J 7: 79–81

    Google Scholar 

  • Van Boxtel JHJ (1994) Studies on genetic transformation of coffee by using electroporation and the biolistic method. PhD Thesis, Agric Univ Wageningen (ISBN 90–5485–315–8)

    Google Scholar 

  • Yasuda T, Fujii Y, Yamaguchi T (1985) Embryogenic callus induction from Coffea arabica leaves by benzyladenine. Plant Cell Physiol 26: 575–597

    Google Scholar 

  • Yasuda T, Tahara M, Uchida N, Yamaguchi T (1986) Somatic embryogenesis from coffee callus and protoplast. In: Somers DA, Gengenbach BG, Biesboer DD, Hackett WP, Green CE (eds) Abstr 6th Int Congr Plant Tissue Cell Culture. Univ Minnesota, Minneapolis, p 137

    Google Scholar 

  • Zamarripa A, Ducos JP, Bollon H, Dufour M, Pétiard V (1991) Production d’embryons somatiques de caféier en milieu liquide: effet densité d’inoculation et renouvellement du milieu. Café Cacao Thé 35: 233–244

    Google Scholar 

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Authors and Affiliations

  1. ILTAB/ORSTOM, The Scripps Research Institute, Division of Plant Biology — MRC7, 10666 North Torrey Pines Road, La Jolla, California, 92037, USA

    C. Schöpke

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  1. C. Schöpke
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  1. A-137 New Friends Colony, 110065, New Delhi, India

    Y. P. S. Bajaj

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© 1996 Springer-Verlag Berlin Heidelberg

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Schöpke, C. (1996). Regeneration of Plants from Protoplasts of Coffea spp. (Coffee). In: Bajaj, Y.P.S. (eds) Plant Protoplasts and Genetic Engineering VII. Biotechnology in Agriculture and Forestry, vol 38. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-09368-9_3

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  • DOI: https://doi.org/10.1007/978-3-662-09368-9_3

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-08240-5

  • Online ISBN: 978-3-662-09368-9

  • eBook Packages: Springer Book Archive

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