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Use of CLPP to Evaluate the Role of Different Organic Materials in Composting

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Microbiology of Composting

Abstract

In this study, changes in the microbial functional diversity during composting were analysed. Seven samples corresponding to (1) three different residues used in composting (sludges, citrus industrial processing waste, green wastes), and (2) four different stages of compost maturity were compared by their “community level physiological profiles” (CLPP). Ecoplates (Biolog) were inoculated with compost and matrices and incubated at 30 °C; optical density was measured every 8 h for 8 days. The samples were compared on the basis of the kinetics of the curves produced in each well. The data were used to calculate the area under the curve, and to study the curve kinetics. As a whole, the CLPP succeeded in addressing the role of different residues in the functional diversity of the mature compost. The microbial physiological profile of the sludge was found in the final product, while no “metabolic” trace of the other two matrices was found. The samples corresponding to different stages of the composting process were well characterised by their CLPPs, showing an increase in the metabolic diversity during the stabilisation process.

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References

  • Canali S, Trinchera A, Pinzari F, Benedetti A (1998) Study of compost maturity by means of humification parameters and isoelectric focusing technique. CD-Rom, Proc 16th World Congr of Soil Science. Montpellier, France 20–26 August 1998

    Google Scholar 

  • Collins CH, Lyne PM, Grange JM (1989) Collins and Lyne’s microbiological methods, 6th edn Butterworths,London

    Google Scholar 

  • Correia Guerrero C, Carrasco de Brito J, Lapa N, Santos Oliveira F (1995) Re-use of industrial orange wastes as organic fertilizers. Biores Technol 53: 43–51

    Article  Google Scholar 

  • Garland JL (1997) Analysis and interpretation of community-level physiological profiles in microbial ecology. FEMS Microb Ecol 24: 289–300

    Article  CAS  Google Scholar 

  • Garland JL, Mills AL (1991) Classification and characterization of heterotrophic microbial communities on the basis of patterns of community-level sole-carbon-source utilization. Appl Environ Microbiol 57: 2351–2359

    PubMed  CAS  Google Scholar 

  • Harch BD, Correl RL, Meech W, Kirkby CA, Pankhurst CE (1997) Using the Gini coefficient with Biolog substrate utilisation data to provide an alternative quantitative measure for comparing bacterial soil communities. J Microbiol Methods 30: 91–101

    Article  CAS  Google Scholar 

  • Hellmann B, Zelles L, Palojarvi A, Bay Q (1997) Emission of climate-relevant trace gases and succession of microbial communities during open-windrow composting. Appl Environ Microbiol 63: 1011–1018

    PubMed  CAS  Google Scholar 

  • Hopkins DW, MacNaughton SJ, O’Donnell AG (1991) A dispersion and differential centrifugation technique for representatively sampling microorganisms from soil. Soil Biol Biochem 23: 217–225

    Article  Google Scholar 

  • Insam H (1997) A new set of substrates proposed for community characterisation in environmental samples. In: Insam H, Rangger A (eds) Microbial communities: functional versus structural approaches. Springer, Berlin Heidelberg New York, pp 259–260

    Google Scholar 

  • Insam H, Rangger A (1997) Microbial communities: functional versus structural approaches. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Insam H, Amor K, Renner M, Crepaz C (1996) Changes in functional abilities of the microbial community during composting of manure. Microb Ecol 31: 77–87

    Article  Google Scholar 

  • Lindstrom JE, Barry RP, Braddock JF (1998) Microbial community analysis: a kinetic approach to constructing potential C-source utilisation pattern. Soil Biol Biochem 30: 231–239

    Article  CAS  Google Scholar 

  • Macdonald RM (1986) Sampling soil microfloras: dispersion of soil by ion exchange and extraction of specific microorganism by elutriation, Soil Biol Biochem 18: 399–406

    Article  Google Scholar 

  • McKinley VL, Vestal JR (1984) Biokinetic analysis of adaptation and succession: microbial activity in composting municipal sewage sludge. Appl Environ Microbiol 47: 933–939

    PubMed  CAS  Google Scholar 

  • Miller FC (1993) Composting as a process based on the control of ecologically selective factors. In: Metting FB (ed) Soil microbial ecology. Marcel Dekker, New York

    Google Scholar 

  • Niepold F, Conrad R, Schlegel HG (1979) Evaluation of the efficiency of extraction for the quantitative estimation of hydrogen bacteria in soil. Antonie van Leewenhoek 45: 485497

    Google Scholar 

  • OriginLab Corporation (1999) Origin version 6.0 - Northampton, MA

    Google Scholar 

  • Sharma S, Piccolo A, Insam H (1997) Different carbon source utilisation profiles of four tropical soils from Ethiopia. In: Insam H, Rangger A (eds) Microbial communities: functional versus structural approaches. Springer, Berlin Heidelberg New York, pp 132–139

    Google Scholar 

  • Sparling GP, Ord BG, Vaughan D (1981) Changes in microbial biomass and activity in soils amended with phenolic acids. Soil Biol Biochem 13: 455–460

    Article  CAS  Google Scholar 

  • SPSS (1998) Statistical package of the Social Sciences. SPSS, Chicago

    Google Scholar 

  • Strom P (1985) Effect of temperature on bacterial species diversity in thermophilic solid-waste composting. Appl Environ Microbiol 50: 899–905

    PubMed  CAS  Google Scholar 

  • Williams AH (1962) Enzyme inhibition by phenolic compounds. In: Pridham JB (ed) Enzyme chemistry of phenolic compounds. Pergamon Press, New York. pp 87–95

    Google Scholar 

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© 2002 Springer-Verlag Berlin Heidelberg

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Pinzari, F., Tittarelli, F., Benedetti, A., Insam, H. (2002). Use of CLPP to Evaluate the Role of Different Organic Materials in Composting. In: Insam, H., Riddech, N., Klammer, S. (eds) Microbiology of Composting. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-08724-4_32

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  • DOI: https://doi.org/10.1007/978-3-662-08724-4_32

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-08705-9

  • Online ISBN: 978-3-662-08724-4

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