Abstract
Since its introduction in 1993, peptide mass fingerprinting (PMF) based on matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-MS) has become a standard method in proteomics for the identification of proteins (Mann, 1993; Yates, 1993; Henzel, 1993; James, 1993; Pappin, 1993). Although a number of parameters like protein size and pl are also used by PMF search programs, the main parameter for obtaining a positive identification is to locate a sufficient number of peptides that match the theoretical peptides in a given protein database.
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Hjernø, K., Højrup, P. (2004). Peak Erazor: A Windows-Based Program for Improving Peptide Mass Searches. In: Kamp, R.M., Calvete, J.J., Choli-Papadopoulou, T. (eds) Methods in Proteome and Protein Analysis. Principles and Practice. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-08722-0_24
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DOI: https://doi.org/10.1007/978-3-662-08722-0_24
Publisher Name: Springer, Berlin, Heidelberg
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