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Cephalocereus senilis (Old-Man-Cactus): In Vitro Culture and the Elicitation of Flavonoids

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Medicinal and Aromatic Plants IX

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 37))

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Abstract

The abundance of cacti in arid lands is, at least in part, due to several biochemical and anatomical adaptations that cacti have for minimizing water loss. To what degree members of the Cactaceae have evolved defenses against microbial attack or insect feeding is not as well studied. In this chapter we will examine how one biosynthetic pathway, the route leading to flavonoid synthesis, responds to conditions which simulate bacterial infection. The chemical and biochemical studies have focused on a popular ornamental species, Cephalocereus senilis (old-man-cactus; Fig. 1A)

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References

  • Barcikowski W, Nobel PS (1984) Water relations of cacti during desiccation: distribution of water in tissue. Bot Gaz 145: 110–115

    Article  Google Scholar 

  • Bonness MS, Paré PW, Mabry TJ (1992) Novel callus and suspension cultures of Cephalocereus senilis (old-man-cactus). Cactus Succulent J 65: 144–147

    Google Scholar 

  • Britton NS, Rose JN (1963) The Cactaceae. Dover, New York

    Google Scholar 

  • Clark WD, Brown GK, Mays RL (1980) Flower flavonoids of Opuntia series Opuntiae. Phytochemistry 19: 1856–1857

    Article  CAS  Google Scholar 

  • Escobar HA, Villalobos VM, Villegas A (1986) Opuntia micropropagation by axillary proliferation. Plant Cell Tissue Organ Cult 7: 269–277

    Google Scholar 

  • Gibson AC, Nobel PS (1986) The cactus primer. Harvard University Press, Cambridge, MA Grand C, Boudet A, Boudet AM (1983) Isoenzymes of hydroxycinnamate: CoA ligase from poplar stems: properties and tissue distribution. Planta 158: 225–229

    Google Scholar 

  • Hain R, Reif HJ, Krause E, Landebartels R, Kindl H, Vornam B, Wiese W, Schmelzer E, Schreier PH, Stocker RH, Stenzel K (1993) Disease resistance results from foreign phytoalexin expression in a novel plant. Nature 361: 153–156

    Article  PubMed  CAS  Google Scholar 

  • Hamburger MO, Cordell GA (1987) A direct bioautographic TLC assay for compounds possessing antibacterial activity. J Nat Prod 50: 19–25

    Article  PubMed  CAS  Google Scholar 

  • Harborne JB (1988) An introduction to ecological biochemistry. Academic Press, London Heinzmann U, Seitz U, Seitz U (1977) Purification and substrate specificities of hydroxycinnamate:

    Google Scholar 

  • CoA ligase from anthocyanin-containing and anthocyanin-free carrot cells. Planta 135: 313–318

    Google Scholar 

  • Heller W, Forkmann, G. (1988) Biosynthesis. In: Harborne JB (ed) The Flavonoids: advances in research since 1980. Chapman and Hall, New York, pp 399–425

    Google Scholar 

  • Hubstenberger JF, Clayton PW, Phillips GC (1992) Micropropagation of cacti (Cactaceae). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 20. High-tech and micropropagation IV. Springer, Berlin Heidelberg New York, pp 49–68

    Google Scholar 

  • Innes C (1987) Cacti. The Royal Horticultural Society. Wing King Tong Co, Hong Kong

    Google Scholar 

  • Kutsuki H, Shimada M, Higuchi T (1982) Distribution and roles of p-hydroxycinnamate:CoA ligase in lignin biosynthesis. Phytochemistry 21: 267–271

    Article  CAS  Google Scholar 

  • Liu Q, Markham KR, Paré PW, Dixon RA, Mabry TJ (1993a) Flavonoids from elicitor-treated cell suspension cultures of Cephalocereus senilis. Phytochemistry 32: 144–147

    Google Scholar 

  • Liu Q, Dixon RA, Mabry TJ (1993b) Additional flavonoids from elicitor-treated cell cultures of Cephalocereus senilis. Phytochemistry 34: 167–170

    Article  CAS  Google Scholar 

  • Liu Q, Liu M, Mabry TJ, Dixon RA (1994) Flavonol glycosides from Cephalocereus senilis. Phytochemistry 36: 229–231

    Article  PubMed  CAS  Google Scholar 

  • Liu Q, Bonness MS, Liu M, Seradge E, Dixon RA, Mabry TJ (1995) Enzymes of flavonoid biosynthesis in elicited cell cultures of “old man” cactus (Cephalocereus senilis) (in preparation)

    Google Scholar 

  • Mabry TJ, Nguyen HT, Dixon RA, Bonness MS (1993) Biotechnology for aridland plants. IC2 Institute, Austin

    Google Scholar 

  • Mann J (1987) Secondary metabolism. Clarendon Press, Oxford, 276 pp

    Google Scholar 

  • Maule AJ, Ride JP (1983) Cinnamate 4-hydroxylase and hydroxycinnamate: CoA ligase in wheat leaves infected with Botrytis cinerea. Phytochemistry 22: 1113–1116

    Article  CAS  Google Scholar 

  • Mauseth JD (1979) A new method for the propagation of cacti: sterile culture of axillary buds. Cactus Succulent J 51: 186–187

    Google Scholar 

  • Mauseth JD, Halperin W (1975) Hormonal control of organogenesis in Opuntia polycantha ( Cactaceae ). Am J Bot 62: 869–877

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant 15: 473–497

    Article  CAS  Google Scholar 

  • Paré PW, Mabry TJ (1993) Chemical defense in Cephalocereus senilis (old-man-cactus) against the cactus soft rot pathogen Erwinia cacticida. Haseltonia 1: 61–64

    Google Scholar 

  • Paré PW, Dmitrieva N, Mabry TJ (1991) Phytoalexin aurone induced in Cephalocereus senilis liquid suspension culture. Phytochemistry 30: 1133–1135

    Article  Google Scholar 

  • Paré PW, Mischke CF, Edwards R, Dixon RA, Norman HA, Mabry TJ (1992) Induction of phenylpropanoid pathway enzymes in elicitor-treated cultures of Cephalocereus senilis. Phytochemistry 31: 149–154

    Article  Google Scholar 

  • Seeni S, Gnanam A (1980) Photosynthesis in cell suspension cultures of the CAM plant Chamaecereus sylvestrii ( Cactaceae ). Physiol Plant 49: 465–472

    Google Scholar 

  • Steinhart CE (1962) Tissue cultures of a cactus. Science 137: 545–546

    Article  PubMed  CAS  Google Scholar 

  • Worchok Z.S. (1981) The role of tissue culture in preserving threatened and endangered plant species. Biol Consery 20: 83–89.

    Article  Google Scholar 

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© 1996 Springer-Verlag Berlin Heidelberg

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Paré, P.W., Liu, Q., Bonness, M.S., Liu, M., Dixon, R.A., Mabry, T.J. (1996). Cephalocereus senilis (Old-Man-Cactus): In Vitro Culture and the Elicitation of Flavonoids. In: Bajaj, Y.P.S. (eds) Medicinal and Aromatic Plants IX. Biotechnology in Agriculture and Forestry, vol 37. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-08618-6_7

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  • DOI: https://doi.org/10.1007/978-3-662-08618-6_7

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-08229-0

  • Online ISBN: 978-3-662-08618-6

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