Blackstonia perfoliata (L.) Hudson (Yellow Wort): In Vitro Culture and the Production of Gentiopicroside and Other Secondary Metabolites

  • L. Skrzypczak
  • M. Wesołowska
  • B. Thiem
  • J. Budzianowski
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 37)


Blackstonia perfoliata (L.) Hudson (Fig. 1) (Chlora perfoliata L., Gentiana perfoliata L., Seguiera perfoliata O. Kuntze) of the family Gentianaceae, is called yellow wort or, yellow centaury from the color of its flowers. The genus Blackstonia is associated with the name of the author (J. Blackstone 1737), who described this plant in England (Hegi 1966). Zeltner (1970) distinguished four species in the genus Blackstonia. Tutin (1972) in Flora Europaea mentioned only one species with four subspecies: perfoliata,serotina (Koch ex Reichenb.) Vollmann, imperfoliata (L. fil.) Franco and Rocha, and grandiflora (Viv.) Maire in Jahandiez and Maire. The former two have the chromosome number 2n = 40, the others 2n = 20. The Flora of Switzerland (Hess et al. 1972) includes two species, B. perfoliata and B. acuminata (Koch et Ziz) Domin (B. serotina, Chlora serotina). These authors reported the chromosome number 2n = 44 in B. perfoliata from England and northern France. Blackstonia perfoliata is an annual, 10–60-cmhigh, erect and glaucous herb, ending in a dichasium with triangular leaves, sometimes narrowing towards the base. The yellow corolla of flowers and the linear or lanceolate calyx are divided into 6–12 lobes (Tutin 1972). The size and the lobe number represent the only differences between the subspecies. The blooming period of the plant is from June to September or from April to August (Feinbrun-Dothan 1978).


Callus Tissue Terephthalic Acid Green Callus Yellow Corolla Loganic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • L. Skrzypczak
  • M. Wesołowska
  • B. Thiem
  • J. Budzianowski
    • 1
  1. 1.Department of Pharmaceutical BotanyK. Marcinkowski University of Medical Sciences in PoznańPoznańPoland

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