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Agave amaniensis Trel & Nowell: In Vitro Culture and the Production of Phytosteroids

  • G. Indrayanto
  • W. Utami
  • A. Syahrani
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 37)

Abstract

Agave amaniensis Trel & Nowell (family Agavaceae) was first found (under the name Agave lespinassei Trel) at the East African Agriculture Research Station, Amani, Tanganyika, in 1929. Its origin was unknown (Nowell 1933; Dahlgren et al. 1985). It is a perennial herb; habit erect, acaulescent, finally suckering rather freely. Leaves ensiform, rigid, leathery, (Fig. lA) ascending except for the short basal ones, which turn down; markedly glaucous on both sides, ground color deep, dull yellow-green, length 1.4–2 m, width at base 16 cm, at waist 10 cm, and at blade 15 cm. Flower and fruit are unknown. Propagation so far has depended on the transplanting of suckers, which are produced after 2 years of growth (Nowell 1933; Jacobsen 1978).

Keywords

Callus Culture Growth Index Plant Cell Tissue Organ Cult Sapogenin Steroid Glutamate Dehydrogenase Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Binh LT, Muoi LT, Oanh HTK, Thang TD, Phong DT (1990) Rapid propagation of Agave by in vitro tissue cultures. Plant Cell Tissue Organ Cult 23: 67–70CrossRefGoogle Scholar
  2. Binh LT, Muoi LT, Hien DT, An NY, Thang TD, Phong DT, Oanh HTK (1993) Proc Southeast Asian Regional Workshop on Propagation Techniques for Commercial Crops of the Tropics, Ho Chi Minh City, Vietnam, IFS, Sweden, pp 118–123Google Scholar
  3. Blunden G, Patel AV (1986) Baarbourgenin, a new steroidal sapogenin from Agave sisalana. J Nat Prod 49: 687–689CrossRefPubMedGoogle Scholar
  4. Blunden G, Yi Yi, Jewers K (1978) Steroidal sapogenins from leaves of Agave species. Phytochemistry 17: 1923–1925CrossRefGoogle Scholar
  5. Blunden G, Carabot A, Jewers K (1980) Steroidal sapogenins from leaves of some species of Agave and Furcraea. Phytochemistry 19: 2489–2490CrossRefGoogle Scholar
  6. Castro-Concha L, Loyola-Vargas VM, Chan JL, Robert ML (1990) Glutamate dehydrogenase activity in normal and vitrified plants of Agave tequilana Weber propagated in vitro. Plant Cell Tissue Organ Cult 22: 147–151CrossRefGoogle Scholar
  7. Dahlgren RMT, Clifford HT, Yeo PF (1985) The families of the Monocotyledons. Springer, Berlin Heidelberg New York, pp 157–161CrossRefGoogle Scholar
  8. Gbolade AA, Elujoba AA, Sofowora A (1985) Steroidal sapogenin content of Agave species cultivated in Nigeria. In: Gorog S (ed) Advances in steroid analysis’84. Elsevier, Amsterdam, pp 93–98Google Scholar
  9. Groenewald EG, Wessels DCJ, Koeleman A (1977) Callus formation and subsequent plant regeneration from seed tissue of an Agave species ( Agavaceae ). Z Pflanzenphysiol 81: 369–373Google Scholar
  10. Hegnauer R (1963) Chemotaxonomie der Pflanzen, Bd 2. Birkhäuser, Basel, pp 25–47Google Scholar
  11. Indrayanto G (1983) Steroide und Triterpene in Zellkulturen. PhD Thesis, University of Tübingen, TübingenGoogle Scholar
  12. Indrayanto G, Worokarti, Endang, Didik (1991) Pengaruh fosfat, kolesterol dan beberapa elisitor terhadap kandungan hekogenin pada kultur Agave amaniensis. Paper presented at National Seminar of Secondary metabolites production from plant tissue cultures. University of Gadjah Mada, Yogyakarta, IndonesiaGoogle Scholar
  13. Indrayanto G, Rahayu L, Utami W, Rahman A (1992) Induksi Pembentukan sapogenin steroid pada kultur Agave amaniensis. Research Report, Airlangga University Research Institute, IndonesiaGoogle Scholar
  14. Indrayanto G, Rahayu L, Rahman A, Noerani PE (1993) Effect of calcium, strontium and magnesium ions on the formation of phytosteroids in callus cultures of Agave amaniensis. Planta Med 59: 97–98CrossRefPubMedGoogle Scholar
  15. Indrayanto G, Studiawan H, Cholies N. (1994) Isolation and quantitation of manogenin and kammogenin from callus cultures of Agave amaniensis. Phytochem Anal 5: 24–26CrossRefGoogle Scholar
  16. Jacobsen H (1978) A handbook of succulent plants. Blandfords Press, Poole Dorset, pp 67–73Google Scholar
  17. Madrigal-Lugo R, Pineda-Estrada F, Rodrigues-Delao JL (1990) Agave In: Ammorato PV, Evans DA, Sharp WR, Bajaj YPS (eds) Handbook of plant cell cultures, vol 5. Ornamental species. McGraw-Hill, New York, pp 206–225Google Scholar
  18. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497CrossRefGoogle Scholar
  19. Nowell W (1933) History and properties of A. amaniensis. Kew Bull 10: 465–467Google Scholar
  20. Nuraini S (1992) Penentuan kadar hekogenin pada beberapa macam Agave spp, secara densitometri. Skripsi, Faculty of Pharmacy, Airlangga University, Surabaya, IndonesiaGoogle Scholar
  21. Patel AV, Blunden G, Crabb TA Savaire Y Baccau YC (1987) A review of naturally occurring steroidal sapogenin. Fitoterapia LVIII: 67–107Google Scholar
  22. Powers DE, Backhaus RA (1989) In Vitro propagation of Agave arizonica Gentry & Weber. Plant Cell Tissue Organ Cult 16: 57–60CrossRefGoogle Scholar
  23. Rana U (1993) Molluscicidal steroid glycoside from Agave cantala. Int J Pharmacognosy 31: 65–67CrossRefGoogle Scholar
  24. Robert ML, Herrera JL, Chan, JL, Contreras F (1992) Micropropagation of Agave spp. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 19. Hi-tech and micropropagation III. Springer, Berlin Heidelberg New York, pp 307–329Google Scholar
  25. Sati OP, Rana U, Chaukiyal DC, Sholichin M (1987) A new spirostanol glycoside from Agave cantala. J Nat Prod 50: 263–265CrossRefGoogle Scholar
  26. Savangikar VA, Tabe RH, Nimbkar N (1989) Micropropagation of Agave sisalana Perrine (Sisal) In: Tissue cultures and biotechnology in aromatics. CIMAP, Lucknow, India, pp 39–43Google Scholar
  27. Schripsema J (1991) Factors involved in the alkaloid production of Tabernaemontana divaricata plant cell suspension cultures. PhD Thesis, University of Leiden, LeidenGoogle Scholar
  28. Schübel H, Ruyter CM, Stockigt J (1989) Improved production of raucaffricine by cultivated Rauwolfia cells. Phytochemistry 28: 491–494CrossRefGoogle Scholar
  29. Setia Dewi (1988) Percobaan penumbuhan kalus Agave amaniensis dan deteksi steroidnya, Skripsi, Faculty of Pharmacy, Airlangga University, Surabaya, IndonesiaGoogle Scholar
  30. Sharma OP Khanna P (1980) Studies on steroidal sapogenin from tissue cultures of Agave wightii. J Nat Prod 43: 459–462CrossRefGoogle Scholar
  31. Sierra MI, Dagnino D, Van der Heijden R, Verpoorte R (1991) Influence of Ca on peroxidase activity and alkaloid formation in Tabernaemontana divaricata cell suspensions cultures, In: Lobarzewski J, Greppin H, Panel C, Gaspar Th. (eds) Biochemical, molecular and physiological aspects of plant peroxidases. University of Geneva, Geneva, pp 295–304Google Scholar
  32. Threfall DR, Whitehead M (1988) The use of metal ions to induce the formation of secondary products in plant tissue culture, In: Robbin RJ, Rhodes MJC (eds) Manipulating secondary metabolism. Cambridge University Press, Cambridge, pp 51–57Google Scholar
  33. Wahyu Utami (1990) Isolasi dan identifikasi steroid pada kalus Agave amaniensis. MSc Thesis, Faculty of Graduate Studies, Airlangga University, IndonesiaGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • G. Indrayanto
  • W. Utami
  • A. Syahrani
    • 1
  1. 1.Laboratory of Pharmaceutical Biotechnology, Faculty of PharmacyAirlangga UniversitySurabayaIndonesia

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