Skip to main content
Book cover

Medicinal and Aromatic Plants XI pp 261–273Cite as

Lactuca virosa L. (Bitter Lettuce): In Vitro Culture and Production of Sesquiterpene Lactones

  • Chapter

  • 408 Accesses

Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE,volume 43)

Abstract

Lactuca virosa L. (family Asteraceae), also known as Lactuca silvestris Lam. or Wiestia virosa C.H. Schultz, is indigenous to south west Europe, North Africa and West Asia, and is also distributed in other regions of the Northern Hemisphere, including America. It grows singly or in clusters, on rocky soil and as a ruderal plant, on plains and uplands up to 1000m above sea level (Hallier 1887; Hegi 1929; Bremer 1994).

Keywords

  • Root Culture
  • Hairy Root Culture
  • Growth Index
  • Sesquiterpene Lactone
  • Coniferyl Alcohol

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

This is a preview of subscription content, access via your institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • DOI: 10.1007/978-3-662-08614-8_15
  • Chapter length: 13 pages
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
eBook
USD   349.00
Price excludes VAT (USA)
  • ISBN: 978-3-662-08614-8
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD   449.99
Price excludes VAT (USA)
Hardcover Book
USD   449.99
Price excludes VAT (USA)
Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Alconero R (1988) Lettuce (Lactuca sativa L.). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 6. Crops II. Springer, Berlin Heidelberg New York, pp 351–369

    Google Scholar 

  • Bachelor FW, Ito S (1973) A revision of the stereochemistry of lactucin. Can J Chem 51: 3626–3630

    CrossRef  CAS  Google Scholar 

  • Barton DHR, Narayanan CR (1958) Sesquiterpenoids. X. The constituents of lactucin. J Chem Soc 963–971

    Google Scholar 

  • Behrens I (1938) Über unsere Erfahrungen mit Latucyl. MMW 85: 139

    Google Scholar 

  • Binek E (1995) Próba uprawy salaty jadowitej w warunkach krajowych. Wiad Ziel 5: 9

    Google Scholar 

  • Bremer K (1994) Asteraceae, cladistics and classification. Timber Press, Portland, Oregon, pp 157–201

    Google Scholar 

  • Da Legnano LP (1968) Le piante medicinali nella cura delle malattie umane. Edizioni Mediterranee, Rome, p 826

    Google Scholar 

  • Dolejs L, Souéek M, Horak M, Herout V, Sorm F (1958) On terpenes. XCIV. The structure of lactucin. Collect Czech Chem Commun 23: 2195–2199

    Google Scholar 

  • Enomoto S, Ohashi Y (1993) Transformation of lettuce (Lactuca sativa L.) In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 33. Plant protoplasts and genetic engineering IV. Springer, Berlin Heidelberg New York, pp 237–247

    Google Scholar 

  • Enomoto S, Ohyama K (1989) Regeneration of plant from protoplasts of lettuce and its wild species. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 8. Plant protoplasts and genetic enginerring I. Springer, Berlin Heidelberg New York, pp 217–226

    Google Scholar 

  • Forst AW (1937) Der Giftlattich, eine vergessene, alte Heilpflanze. MMW 84: 1251–1254

    Google Scholar 

  • Fournet A, Munoz V, Robbt F, Hoequemiller R, Cave A, Gantier J-C (1993) Antiprotozoal activity of dehydrozaluzanin C, a sesquiterpene lactone isolated from Munnozia maronii ( Asteraceae ). Phytother Res 7: 111–115

    Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151–158

    CrossRef  PubMed  CAS  Google Scholar 

  • Gromek D (1989a) Sesquiterpene lactones of Lactuca virosa L. PhD Thesis, Pharmaceutical Faculty, Medical Academy of Cracow (in Polish)

    Google Scholar 

  • Gromek D (1989b) Sesquiterpene lactones from Lactuca virosa L. Pol J Chem 63:297–301 Gromek D (1991) Lactuside A from Lactuca virosa L. Pol J Chem 65: 11–12

    Google Scholar 

  • Gromek D, Kisiel W, Klodzinska A, Chojnacka-Wójcik E (1992) Biologically active preparations from Lactuca virosa L. Phytother Res 6: 285–287

    CrossRef  CAS  Google Scholar 

  • Hallier E (1887) Flora von Deutschland, vol 30. Köhler, Gera-Untermhaus, pp 266–268

    Google Scholar 

  • Hegi G (1929) Illustrierte Flora von Mittel-Europa, vol 6. Lehmanns Munich, pp 122–125

    Google Scholar 

  • Hegnauer R (1964) Chemotaxonomie der Pflanzen, vol 3. Birkhäuser, Basel

    Google Scholar 

  • Hegnauer R (1989) Chemotaxonomie der Pflanzen, vol 8. Birkhäuser, Basel

    Google Scholar 

  • Hessen W (1937) Latucyl, ein neues Hustenbekämpfungsmittel. Dtsch Med Wochenschr 62: 17311732

    Google Scholar 

  • Holzer K, Zinke A (1953) Über die Bitterstoffe der Zichorie (Cichorium intybus L.). II. Mitteilung: Lactucin und Lactucopikrin. Monatsh Chem 84: 901–909

    Google Scholar 

  • Hooykaas PJJ, Klapwijk PM, Nuti MP, Schilperoort RA, Rorsch A (1977) Transfer of the Agrobacterium tumefaciens Ti plasmid to virulent Agrobacteria and to Rhizobium explanta. J Gen Microbiol 98: 477–484

    CrossRef  Google Scholar 

  • Jolad SD, Wiedhopf RM, Cole JR (1974) Tumor-inhibitory agent from Zaluzania robinsonii ( Compositae ). J Pharmacol Sci 63: 1321–1322

    Google Scholar 

  • Kisiel W, Barszcz B (1997) Minor sesquiterpene lactones from Lactuca virosa. Phytochemistry 46: 1241–1243

    CAS  Google Scholar 

  • Kisiel W, Stojakowska A, Malarz J, Kohlmünzer S (1995) Sesquiterpene lactones in Agrobacterium rhizogenes-transformed hairy root culture of Lactuca virosa. Phytochemistry 40: 1139–1140

    CrossRef  CAS  Google Scholar 

  • Kohlmünzer S, Grzybek J, Kisiel W (1976) Biological activity of vernoflexuoside on the basis of Allium test. Experientia 32: 172–173

    CrossRef  Google Scholar 

  • Levis WH, Elvin-Levis MPF (1977) Medical botany, plants affecting man’s health. Wiley, London, p 446

    Google Scholar 

  • List PH, Hörhammer L (1973) Hagers Handbuch der pharmazeutischen Praxis, vol 5. Springer, Berlin Heidelberg New York, pp 432–434

    Google Scholar 

  • Malarz J (1997) Optimization of sesquiterpene lactone biosynthesis in hairy root culture of Lactuca virosa L. PhD Thesis, Pharmaceutical Faculty, Collegium Medicum, Jagiellonian University, Cracow (in Polish)

    Google Scholar 

  • Morgan AJ, Cox PN, Turner DA, Peel E, Davey MR, Gartland KMA, Mulligan BJ (1987) Transformation of tomato using an Ri plasmid vector. Plant Sci 49: 37–49

    CrossRef  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant 15: 473–497

    CrossRef  CAS  Google Scholar 

  • Pink DAC (1992) Micropropagation of lettuce (Lactuca sativa L.). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 19. High-Tech and micropropagation III. Springer, Berlin Heidelberg New York, pp 42–57

    Google Scholar 

  • Pyrek JS (1977) Terpenes of Compositae plants. V. Sesquiterpene lactones of Lactuca serriola L. The structure of 8-deoxylactucin and the site of esterification of lactupicrin. Rocz Chem 51: 2165–2170

    Google Scholar 

  • Schripsema J, Verpoorte R (1995) Novel techniques for growth characterization and phytochemical analysis of plant cell suspension cultures. J Nat Prod 58: 1305–1314

    CrossRef  CAS  Google Scholar 

  • Schripsema J, Meijer AH, Van Iren F, Ten Hoopen HJG, Verpoorte R (1990) Dissimilation curves as a simple method for the characterization of growth of plant cell suspension cultures. Plant Cell Tissue Organ Cult 22: 55–64

    Google Scholar 

  • Seto M, Miyase T, Umehara K, Ueno A, Hirano Y, Otani N (1988) Sesquiterpene lactones from Cichorium endivia L. and C. intybus L. and cytotoxic activity. Chem Pharm Bull (Tokyo) 36: 2423–2429

    CrossRef  PubMed  CAS  Google Scholar 

  • Song Q, Gomez-Barrios ML, Hopper EL, Hjortso MA, Fisher NH (1995) Biosynthetic studies of lactucin derivatives in hairy root culture of Lactuca floridana. Phytochemistry 40: 1659–1665

    CrossRef  CAS  Google Scholar 

  • Späth E, Lorenz R, Kuhn H (1951) Über das Lactucin (I. Mitteilung ). Monatsh Chem 82: 114–121

    Google Scholar 

  • Stojakowska A, Malarz J, Kisiel W (1994) Sesquiterpene lactones in tissue culture of Lactuca virosa. Planta Med 60: 93–94

    CrossRef  PubMed  CAS  Google Scholar 

  • Stojakowska A, Malarz J, Van der Heijden R, Verpoorte R (1997) Accumulation of ixerin F and activities of some terpenoid biosynthetic enzymes in a cell suspension culture of Lactuca virosa L. Acta Soc Bot Pol 66: 185–188

    CrossRef  CAS  Google Scholar 

  • Tamaki H, Robinson RW, Anderson JL, Stoewsand GS (1995) Sesquiterpene lactones in virus-resistant lettuce. J Agric Food Chem 43: 6–8

    CrossRef  CAS  Google Scholar 

  • Van Beek TA, Maas P, King BM, Leclercq E, Voragen AGJ, De Groot A (1990) Bitter sesquiterpene lactones from chicory roots. J Agric Food Chem 38: 1035–1038

    Google Scholar 

  • Weiss RF (1974) Lehrbuch der Phytotherapie. Hippokrates, Stuttgart, p 217

    Google Scholar 

  • Wessely F, Lorenz R, Kuhn H (1951): Über das Lactucin (II. Mitteilung ). Monatsh Chem 82: 322–329

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Pharmacology, Polish Academy of Science, 12 Smetna Street, 31-343, Krakow, Poland

    A. Stojakowska, J. Malarz & W. Kisiel

Authors
  1. A. Stojakowska
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Malarz
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. W. Kisiel
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Biotechnology in Agriculture and Forestry, A-137 New Friends Colony, 110065, New Delhi, India

    Professor Dr. Y. P. S. Bajaj

Rights and permissions

Reprints and Permissions

Copyright information

© 1999 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Stojakowska, A., Malarz, J., Kisiel, W. (1999). Lactuca virosa L. (Bitter Lettuce): In Vitro Culture and Production of Sesquiterpene Lactones. In: Bajaj, Y.P.S. (eds) Medicinal and Aromatic Plants XI. Biotechnology in Agriculture and Forestry, vol 43. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-08614-8_15

Download citation

  • DOI: https://doi.org/10.1007/978-3-662-08614-8_15

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-08363-1

  • Online ISBN: 978-3-662-08614-8

  • eBook Packages: Springer Book Archive