Zusammenfassung
Im formalinfixierten, in Paraffin eingebetteten Gewebe hängt der Erfolg immunhistochemischer Färbungen von verschiedenen Bedingungen ab:
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Die nachzuweisenden antigenen Determinanten (Epitope) müssen die Formalinfixierung und den nachfolgenden Einbettungsvorgang unbeschadet überstehen oder sie sollten nach fixierungsbedingter Alteration ihrer tertiären Proteinstruktur rekonstituierbar sein (Antigendemaskierung).
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Erforderlich sind Antikörper, die sich auch im fixierten Gewebe spezifisch an Epitope binden können.
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Zum Nachweis der spezifischen Antikörperbindung sind sensitive Detektionssysteme notwendig, die lichtmikroskopisch erkennbare, permanente Farbsignale geben.
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Kutzner, H., Palmedo, G. (2003). Immunhistologische Techniken. In: Kerl, H., Garbe, C., Cerroni, L., Wolff, H.H. (eds) Histopathologie der Haut. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-07784-9_2
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