Abstract
Since the advent of Agrobacterium transformation of cereal crop species via the “super binary” system (Hiei et al. 1994), direct DNA delivery has gone somewhat “out of vogue” for transgenic maize production. The simplicity of application, the range of available options relative to target tissue, and most importantly, the quality of the subsequent transgenic events with respect to simple integration make Agrobacterium the current transformation method of choice for maize (Ishida et al. 1996). Nonetheless, direct DNA delivery is still a viable option for transgenic maize production (Petolino 2002) as well as for nontransgenic nucleic acid-based gene modification (Zhu et al. 1999).
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Petolino, J.F., Welter, M., Cai, C.Q. (2003). WHISKERS-Mediated Transformation of Maize. In: Jackson, J.F., Linskens, H.F. (eds) Genetic Transformation of Plants. Molecular Methods of Plant Analysis, vol 23. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-07424-4_9
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DOI: https://doi.org/10.1007/978-3-662-07424-4_9
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