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Analysis of DNA from a Specific Chromosome Region

  • Conference paper
Chromosome 12 Aberrations in Human Solid Tumors

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Abstract

Finding the locus of a gene when its mRNA or gene product is known is possible but tedious. On the other hand, isolating a gene from the cytogenetically determined chromosomal locus of a disease (positional cloning or, formerly, reverse genetics; Collins 1992) has so far been successful only in very few cases. Although the Human Genome Mapping Catalogue HGM 11 lists almost 2000 cloned genes, knowledge of the architecture of the human genome is still sporadic and statistical. This is illustrated by the fact that the haploid DNA molecule of a human cell is in total almost 1m long, and that the total DNA in the human body if aligned one after the other is sufficient to cover the distance between earth and sun approximately 80 times. Even a small chromosome band contains a few megabases of DNA with an unpacked length in the order of millimeters, while the longest piece of DNA (C. elegans) which has been completely sequenced, comprises only about 122 kb (Sulston et al. 1992). Thus it becomes clear that we are far from the goal of systematically analyzing the human genome. It may become possible in the not too distant future to systematically analyze a selected chromosome band or a chromosome segment containing very few bands. For this purpose techniques must be provided to isolate DNA selectively from such a chromosome segment.

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Authors and Affiliations

  1. Phys.-Chem. Institut, Universität Heidelberg, Im Neuenheimer Feld 253, 69121, Heidelberg, Germany

    N. Ponelies, J. Scheef, M. Köllner, N. Endlich & K. O. Greulich

  2. Molekulare Genetik, Universität Heidelberg, Im Neuenheimer Feld 236, 69121, Heidelberg, Germany

    N. Ponelies

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  1. N. Ponelies
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  2. J. Scheef
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  3. M. Köllner
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  4. N. Endlich
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  5. K. O. Greulich
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  1. Zentrum f. Humangenetik u. Genetische Beratung, Universität Bremen, Leobener Straße, 28359, Bremen, Germany

    J. Bullerdiek  & S. Bartnitzke  & 

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© 1994 Springer-Verlag Berlin Heidelberg

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Ponelies, N., Scheef, J., Köllner, M., Endlich, N., Greulich, K.O. (1994). Analysis of DNA from a Specific Chromosome Region. In: Bullerdiek, J., Bartnitzke, S. (eds) Chromosome 12 Aberrations in Human Solid Tumors. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-06255-5_12

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  • DOI: https://doi.org/10.1007/978-3-662-06255-5_12

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