Abstract
The genetic map of diploid alfalfa was generated based on the genetic analyses of genotypes of markers collected for the individuals of an F2 population originating from the cross of two diploid alfalfa subspecies, M. sativa ssp. quasifalcata and M. sativa ssp. coerulea (Kiss et al. 1993). The constructed linkage map composed of eight linkage groups which contain more than 1800 morphological, allozyme, and DNA-based markers, of which more than 300 represent loci with known function or sequences in the genome (Kaló et al. 2000). The markers cover approximately 760cM genetic distance. The average correlation between the physical and the genetic distance is about 1000kb/cM. This genetic system was used to map protein markers originating from nodule and seed tissue.
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References
Bloom H, Beier H, Gross HS (1987) Improved silver staining of plant proteins, RNA and DNA in Polyacrylamide gels. Electrophoresis 8:93–99
Coulter EM, Bewley JD (1990) Characterization of a small sulphur-rich storage albumin in seeds of alfalfa (Medicago sativa L.). J Exp Bot 41:1541–1547
Domoney C, Casey R (1983) Cloning and characterization of complementary DNA for vicilin, a major seed storage protein in Pisum sativum L. Planta 159:446–453
Domoney C, Casey R (1985) Measurement of gene number for seed storage proteins in Pisum. Nucleic Acid Res 13:687–699
Ellis THN, Domoney C, Castleton J, Cleary W, Davies DR (1986) Vicilin genes of Pisum. Mol Gen Genet 205:164–169
Gerber S, Rodolphe F, Bahrman N, Baradat P (1993) Seed-protein in maritime pine (Pinus pinaster Ait.) revealed by two-dimensional electrophoresis: genetic determinism and construction of a linkage map. Theor Appl Genet 85:521–528
Higgins TJV (1984) Synthesis and regulation of major proteins in seeds. Annu Rev Plant Physiol 35:191–221
Kaló P, Endre G, Zimányi L, Csanádi G, Kiss GB (2000) Construction of an improved linkage map of diploid alfalfa (Medicago sativa). Theor Appl Genet 100:641–657
Kiss GB, Csanádi G, Kaiman K, Kaló P, Ökrész L (1993) Construction of a basic genetic map for alfalfa using RFLP RAPD isozyme and morphological markers Mol Gen Genet 238:129–137
Kiss GB, Kereszt A, Kiss P, Endre G (1998) Colormapping: a non-mathematical procedure for genetic mapping. Acta Biol Hung 49:125–142
Krochko JE, Bewley JD (1990) Identification and characterization of the seed storage proteins from alfalfa. J Exp Bot 41:505–514
Krochko JE, Bewley JD (2000) Seed storage proteins in cultivars and subspecies of alfalfa (Medicago sativa L.). Seed Sci Res 10:423–434
Krochko JE, Charbonneau MR, Coulter KM, Bowley SR, Bewley JD (1990) A comparison of seed storage proteins in subspecies and cultivars of Medicago sativa. Can J Bot 68:940–948
Leonardi A, Damerval C, De Vienne D (1988) Organ-specific variability and inheritance of maize proteins revealed by two-dimensional electrophoresis. Genet Res Camb 52:97–103
O’Farrell PH (1975) High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250:4007–4021
Stuart DA, Nelsen J (1988) Isolation and characterization of alfalfa 7S and US seed storage proteins. J Plant Physiol 132:129–133
Szende K, Ördögh F (1960) Die Lysogenie von Rhizobium meliloti. Naturwissenshaften 47:404–405
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© 2003 Springer-Verlag Berlin Heidelberg
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Kaló, P., Felföldi, K., Seres, A., Endre, G., Kiss, G.B. (2003). Genetic Mapping of Seed and Nodule Protein Markers in Diploid Alfalfa (Medicago sativa) . In: Nagata, T., Tabata, S. (eds) Brassicas and Legumes From Genome Structure to Breeding. Biotechnology in Agriculture and Forestry, vol 52. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-05036-1_15
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DOI: https://doi.org/10.1007/978-3-662-05036-1_15
Publisher Name: Springer, Berlin, Heidelberg
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