The Development and Implementation of Bioanalytical Methods Using LC-MS to Support ADME Studies in Early Drug Discovery and Candidate Selection
The dramatic improvement in bioanalytical method development and application within the pharmaceutical industry began in the early 1990s with the commercial availability of liquid chromatography-mass spectrometry (LC-MS) systems. As the power of this type of detection was realized, research groups [drug metabolism, safety assessment, product research and development (PR&D), pharmacology, medicinal chemistry] throughout the industry acquired this technology insatiably. Companies purchased LC-MS systems from a variety of vendors and incorporated this technology into their respective areas of research in a number of innovated ways. Clearly, this technology has become the primary analytical tool within the pharmaceutical industry and its incorporation and efficient use have helped to accelerate the generation of high-quality data in support of research and development. By the end of the last century, essentially every bioanalytical department in a pharmaceutical company possessed a number of mass spectrometers, including single-stage, triple quadrupole, time-of-flight (TOF) and ion trap instruments. In addition, many of these groups also purchased automated liquid handling instruments and incorporated them into a variety of sample preparation procedures that are carried out daily in laboratories around the world.
KeywordsBioanalytical Method Electro Spray Ionization Early Drug Discovery Cassette Dose Bioanalytical Laboratory
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