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The Heterotrimeric G Protein Genes of Caenorhabditis elegans

  • Conference paper
Of Fish, Fly, Worm, and Man

Part of the book series: Ernst Schering Research Foundation Workshop ((SCHERING FOUND,volume 29))

Abstract

Caenorhabditis elegans is the first animal, and the first multicellular organism, for which the complete genomic sequence has been determined (The C. elegans Sequencing Consortium 1998). One of the new possibilities in post-sequence genetics is the immediate analysis of complete gene families. A first approximation analysis of gene function involves the determination of expression patterns, and the description of loss-of-function as well as gain-of-function phenotypes. We performed such studies for the complete family of G protein α subunits.

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References

  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215: 403–410

    PubMed  CAS  Google Scholar 

  • Bargmann CI, Horvitz HR (1991) Chemosensory neurons with overlapping functions direct chemotaxis to multiple chemicals in C. elegans. Neuron 7: 729–742

    Article  PubMed  CAS  Google Scholar 

  • Bargmann CI, Hartwieg E, Horvitz HR (1993) Odorant-selective genes and neurons mediate olfaction in C. elegans. Cell 74: 515–527

    Article  PubMed  CAS  Google Scholar 

  • Bargmann CI, Mori I (1997) Chemotaxis and thermotaxis. In: Riddle DL, Blumenthal T, Meyer BJ, Priess JR (eds) C. elegans II. Cold Spring Harbor Press, New York, pp 717–737

    Google Scholar 

  • Brundage L, Avery L, Katz A, Kim U-J, Mendel JE, Sternberg PW, Simon MI (1996) Mutations in a C. elegans Gqa gene disrupt movement, egg-laying and viability. Neuron 16: 999–1009

    Article  PubMed  CAS  Google Scholar 

  • Buck LB (1996) Information coding in the vertebrate olfactory system. Annu Rev Neurosci 19: 517–544

    Article  PubMed  CAS  Google Scholar 

  • Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC (1994) Green fluores-cent protein as a marker for gene expression. Science 263: 802–805

    Article  PubMed  CAS  Google Scholar 

  • Colbert HA, Bargmann CI (1995) Odorant-specific adaptation pathways gen-erate olfactory plasticity in C. elegans. Neuron 14: 803–812

    Article  PubMed  CAS  Google Scholar 

  • Culotti JG, Russell RL (1978) Osmotic avoidance defective mutants of the nematode Caenorhabditis elegans. Genetics 90: 243–256

    PubMed  CAS  Google Scholar 

  • Eeckman FH, Durbin R (1995) ACeDB and Macace. Methods Cell Biol 48: 583–605

    Article  PubMed  CAS  Google Scholar 

  • Fino Silva I, Plasterk RHA (1990) Characterization of a G protein a-subunit gene from the nematode Caenorhabditis elegans. J Mol Biol 215: 483–487

    Article  CAS  Google Scholar 

  • Han M, Sternberg PW (1991) Analysis of dominant negative mutations of the Caenorhabditis elegans let-60 ras gene. Genes Dev 5: 2188–2198

    Article  PubMed  CAS  Google Scholar 

  • Jansen G, Hazendonk E, Thijssen KL, Plasterk RHA (1997) Reverse genetics by chemical mutagenesis in Caenorhabditis elegans. Nature Gen 17: 119–121

    Article  CAS  Google Scholar 

  • Jansen G, Thijssen KL, Werner P, van der Horst M, Hazendonk E, Plasterk RHA (1999) The complete family of genes encoding G proteins of Caenorhabditis elegans. Nature Gen 21: 414–419

    Article  CAS  Google Scholar 

  • Kaplan JM, Horvitz HR (1993) A dual mechanosensory and chemosensory neuron in Caenorhabditis elegans. Proc Natl Acad Sci USA 90: 2227–2231

    Article  PubMed  CAS  Google Scholar 

  • Korswagen HC, Park J-H, Ohshima Y, Plasterk RHA (1997) An activating mutation in a Caenorhabditis elegans Gs protein induces neuronal degeneration. Genes Dev 11: 1493–1503

    Article  PubMed  CAS  Google Scholar 

  • Lochrie MA, Mendel JE, Sternberg PW, Simon MI (1991) Homologous and unique G protein alpha subunits in the nematode Caenorhabditis elegans. Cell Regul 2: 135–154

    PubMed  CAS  Google Scholar 

  • Mello CC, Fire A (1995) DNA transformation. Methods Cell Biol 34:451–482 Mendel JE, Korswagen HC, Liu KS, Hajdu-Cronin YM, Simon MI, Plasterk

    Google Scholar 

  • RHA, Sternberg PW (1995) Participation of the Go protein in multiple as-pects of behavior in C. elegans. Science 267: 1652–1655

    Article  Google Scholar 

  • Neer EJ (1995) Heterotrimeric G proteins: organizers of transmembrane signals. Cell 80: 249–257

    Article  PubMed  CAS  Google Scholar 

  • Park J-H, Ohshima S, Tani T, Ohshima Y (1997) Structure and expression of the gsa-1 gene encoding a G protein a(s) subunit in C. elegans. Gene 194: 183–190

    Article  PubMed  CAS  Google Scholar 

  • Perkins LA, Hedgecock EM, Thomson JN, Culotti JG (1986) Mutant sensory cilia in the nematode Caenorhabditis elegans. Dev Biol 117: 456–487

    Article  PubMed  CAS  Google Scholar 

  • Roayaie K, Gage Crump J, Sagasti A, Bargmann CI (1998) The Ga protein ODR-3 mediates olfactory and nociceptive function and controls cilium morphogenesis in C. elegans olfactory neurons. Neuron 20: 55–67

    Article  PubMed  CAS  Google Scholar 

  • Ségalat L, Elkes DA, Kaplan JM (1995) Modulation of serotonin controlled behaviors by Go in Caenorhabditis elegans. Science 267: 1648–1651

    Article  PubMed  Google Scholar 

  • Sengupta P, Chou JH, Bargmann CI (1994) odr-10 Encodes a seven transmembrane domain olfactory receptor required for responses to the odorant diacetyl. Cell 84: 899–909

    Google Scholar 

  • The C. elegans Sequencing Consortium (1998) Genome sequence of the nematode C. elegans: a platform for investigating biology. Science 282: 2012–2018

    Article  Google Scholar 

  • Troemel E.R, Chou JH, Dwyer ND, Colbert HA, Bargmann CI (1995) Divergent seven transmembrane receptors are candidate chemosensory receptors in C. elegans. Cell 83: 207–218

    Article  PubMed  CAS  Google Scholar 

  • Troemel ER, Kimmel BE, Bargmann CI (1997) Reprogramming chemotaxis responses: sensory neurons define olfactory preferences in C. elegans. Cell 91: 161–169

    Article  PubMed  CAS  Google Scholar 

  • van der Voorn L, Gebbink M, Ploegh HL (1990) Characterization of a G protein (3-subunit from the nematode Caenorhabditis elegans. J Mol Biol 213: 17–26

    Article  PubMed  Google Scholar 

  • Ward S (1973) Chemotaxis by the nematode Caenorhabditis elegans: Identification of attractants and analysis of the response by use of mutants. Proc Natl Acad Sci USA 70: 817–821

    Article  PubMed  CAS  Google Scholar 

  • White JG, Southgate E, Thomson JN, Brenner S (1986) The structure of the nervous system of the nematode Caenorhabditis elegans. Philos Trans R Soc Lond B Biol Sci314: 1–340

    Google Scholar 

  • Yu S, Avery L, Baude E, Garbers DL (1997) Guanylyl cyclase expression in specific sensory neurons: a new family of chemosensory receptors. Proc Natl Acad Sci USA 94: 3384–3387

    Article  PubMed  CAS  Google Scholar 

  • Zwaal RR, Broeks A, van Meurs J, Groenen JTM, Plasterk RHA (1993) Target selected gene inactivation in by using a frozen transposon insertion mutant bank. Proc Natl Acad Sci USA 90: 7431–7435

    Article  PubMed  CAS  Google Scholar 

  • Zwaal RR, Ahringer J, van Luenen HGAM, Rushfort A, Anderson P, Plasterk RHA (1996) G proteins are required for spatial orientation of early cell cleavage in C. elegans embryos. Cell 86: 1–20

    Article  Google Scholar 

  • Zwaal RR, Mendel JE, Sternberg PW, Plasterk RHA (1997) Two neuronal G proteins are involved in the chemosensation of dauer inducing pheromone by C. elegans. Genetics 145: 715–727

    PubMed  CAS  Google Scholar 

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Authors
  1. G. Jansen
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  2. K. L. Thijssen
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  3. P. Werner
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  4. M. van der Horst
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  5. E. Hazendonk
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  6. R. H. A. Plasterk
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Editor information

Editors and Affiliations

  1. Abteilung Genetik, Max-Planck-Institut für Entwicklungsbiologie, Spemannstrasse 35/III, 72076, Tübingen, Germany

    Christiane Nüsslein-Volhard

  2. Enabling Technologies, Preclinical Drug Research, Schering AG, Müllerstrasse 178, 13342, Berlin, Germany

    Jörn Krätzschmar

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© 2000 Springer-Verlag Berlin Heidelberg

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Jansen, G., Thijssen, K.L., Werner, P., van der Horst, M., Hazendonk, E., Plasterk, R.H.A. (2000). The Heterotrimeric G Protein Genes of Caenorhabditis elegans. In: Nüsslein-Volhard, C., Krätzschmar, J. (eds) Of Fish, Fly, Worm, and Man. Ernst Schering Research Foundation Workshop, vol 29. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-04264-9_2

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  • DOI: https://doi.org/10.1007/978-3-662-04264-9_2

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-04266-3

  • Online ISBN: 978-3-662-04264-9

  • eBook Packages: Springer Book Archive

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