Abstract
Aesculus hippocastanum L. (Horse chestnut, Hippocastanaceae) is an extremely variable species with a number of varieties differing in growth habit, leaf shape, and flower features. It is a tree up to 25–30 m high with opposing, long-stalked leaves, composed of five to nine palmate leaflets. The varicolored flowers are borne in showy, upright, terminal, branched clusters. The fruit is a large, round, spiny, fleshy capsule that can be opened by two to three valves. It contains one to four smooth, shiny brown, globular seeds with a prominent, grayish hilum that have no endosperm. The embryo has two large, differently sized cotyledons; from these, a sheath arises surrounding the seedling which is placed in a hollow between the two cotyledons.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Chalupa V (1990) Somatic embryogenesis and plant regeneration in Quercus petraea (Matt.) Lieb. Tilia platyphyllos Scop., and Aesculus hippocastanum L. Lestnictvi. Forestry 36: 599–604
Dameri RM, Caffaro L, Gastaldo P, Profumo P (1986) Callus formation and embryogenesis with leaf explants of Aesculus hippocastanum L. J Plant Physiol 126: 93–96
Jörgensen J (1989) Somatic embryogenesis in Aesculus hippocastanum L. by culture of filament callus. J Plant Physiol 135: 240–241
Kiss J, Heszky LE, Kiss E, Gyulai G (1992) High efficiency adventive embryogenesis on somatic embryos of anther, filament and immature proembryo origin in horse-chestnut (Aesculus hippocastanum L.) tissue culture. Plant Cell Tissue Organ Cult 30: 59–64
List A, Steward FC (1965) The nucellus, embryo sac, endosperm, and embryo of Aesculus hippocastanum and their interdependence during growth. Ann Bot 29 New Ser (113): 1–14
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol Plant 15: 473–497
Profumo P, Dameri RM, Cremona Orsino I (1976) Frammenti cotiledonari di Aesculus hippocastanum L. coltivati in vitro. Primi dati sul comportamento dell’amido e dell’escina. G Bot Ital 110: 155–171
Profumo P, Dameri Orsino RM, Modenesi P (1980) Aescin content in callus from explants of Aesculus hippocastanum L. cotyledons grown in vitro. G Bot Ital 114: 25–28
Profumo P, Gastaldo P, Dameri RM, Caffaro L (1986) Histological study of calli and embryoids from leaf explants of Aesculus hippocastanum L. J Plant Physiol 126: 97–103
Profumo P, Gastaldo P, Martinucci R (1987a) Variations in aescin content in Aesculus hippocastanum seeds during the year. Fitoterapia 58 (3): 184–186
Profumo P, Gastaldo P, Rascio N (1987b) Ultrastructural study of different types of callus from leaf explants of Aesculus hippocastanum. Protoplasma 138: 89–97
Profumo P, Gastaldo P, Dameri RM (1989) Studio preliminare sui rapporti cotiledoni-asse embrionale in Aesculus hippocastanum L. Boll Soc Ital Biol Sper 65: 603–608
Profumo P, Gastaldo P, Caviglia AM, Dameri RM (1990) Somatic embryogenesis from cotyledonary explants of Aesculus hippocastanum L. Acta Embryol Morphol Exp New Ser 11 (2): 101–106
Profumo P, Caviglia AM, Gastaldo P, Dameri RM (199la) Aescin content in embryogenic callus and in embryoids from leaf explants of Aesculus hippocastanum. Planta Med 57: 50–52
Profumo P, Gastaldo P, Bevilacqua L, Carli S (1991b) Plant regeneration from cotyledonary explants of Aesculus hippocastanum L. Plant Sci 76: 139–142
Radojevié L (1978) In vitro induction of androgenic plantlets in Aesculus hippocastanum. Protoplasma 96: 369–374
Radojevic L (1980) Embryogenèse somatique et androgenèse chez certaines espèces ligneuses. Bull Soc Bot Fr 127. Actual Bot (3/4): 99–107
Radojevic L (1988) Plant regeneration of Aesculus hippocastanum L. (horse chestnut) through somatic embryogenesis. J Plant Physiol 132: 322–326
Radojevié L (1991) Horse chestnut (Aesculus spp.) In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 16. Trees III. Springer, Berlin Heidelberg New York, pp 111–141
Radojevié L, Zylberberg L, Kovoor J (1980) Etude ultrastructurale des embryons androgénétiques d’Aesculus hippocastanum L. Z Pflanzenphysiol 98: 255–261
Saito A (1980) In vitro differentiation of embryoid from somatic callus tissues in Aesculus. J Jpn For Soc 62: 308–310
Trippi VS (1963) Studies on ontogeny and senility in plants. II. Seasonal variation in proliferative capacity in vitro of tissues from branches from juvenile and adult zones of Aesculus hippocastanum and Castanea vulgaris. Phyton 20 (2): 146–152
Tutin TG, Heywood VH, Burges NA, Moore DM, Valentine DH, Walters SM, Webb DA (1968) Flora Europaea, vol 2. Cambridge University Press, Cambridge
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1995 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Profumo, P., Gastaldo, P. (1995). Somatic Embryogenesis in Horse Chestnut (Aesculus hippocastanum L.). In: Bajaj, Y.P.S. (eds) Somatic Embryogenesis and Synthetic Seed I. Biotechnology in Agriculture and Forestry, vol 30. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-03091-2_16
Download citation
DOI: https://doi.org/10.1007/978-3-662-03091-2_16
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-08183-5
Online ISBN: 978-3-662-03091-2
eBook Packages: Springer Book Archive