Skip to main content
SpringerLink
Log in

The Study of Biomolecules on Surfaces Using the Scanning Force Microscope

  • Chapter
Studying Cell Adhesion

Abstract

The invention of the Scanning Tunnelling Microscope in 1981 (Binnig et al. 1982) started the development of a whole class of new techniques, all based on probing surface properties by laterally scanning a sharp tip in the proximity of a surface. One of the most promising of these methods for biological applications is Scanning Force Microscopy, SFM (also known as Atomic Force Microscopy, AFM; Binnig et al. 1986). This technique, which has been commercially available for several years1 has some specific features that make it attractive for biological measurements:

  • It has potentially very high resolution (sub nm range)

  • It can image non-conducting species and does not need conducting substrates

  • It can operate in liquids like physiological buffer

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Binnig G, Rohrer H, Gerber C, Weibel E (1982) Surface studies by scanning tunneling microscopy. Phys Rev Lett 49: 57–61

    Article  Google Scholar 

  • Binnig G, Quate C, Gerber C (1986) Atomic force microscope. Phys Rev Lett 56: 930–933

    Article  PubMed  Google Scholar 

  • Bottomley LA, Haseltine JN, Allison DP, Warmack RJ, Thundat T, Sachleben RA, Brown GM, Woychik RP, Jacobson KB, Ferrell TI, Lucovsky G (1992) Scanning Tunneling microscopy of DNA: the chemical modification of gold surfaces for immobilization of DNA. J Vac Sci Technol A 10 (4): 591–595

    Article  CAS  Google Scholar 

  • Bustamante C, Vesenka J, Tang CL, Rees W, Guthold M, Keller R (1992) Circular DNA molecules imaged in air by scanning force microscopy. Biochemistry 31: 22–26

    Article  PubMed  CAS  Google Scholar 

  • Butt HJ, Dowing KH, Hansma PK (1990) Imaging the membrane protein bacteriorhodopsin with the atomic force microscope. Biophys J 58: 1473–1480

    Article  PubMed  CAS  Google Scholar 

  • Drake B, Prater CB, Weisenhorn AL, Gould SAC, Albrecht TR, Quate CF, Cannell DS, Hansma HG, Hansma PK (1989) Imaging crystals, polymers, and processes in water with the atomic force microscope. Science 243: 1586–1589

    Article  PubMed  CAS  Google Scholar 

  • Ducker WA, Senden TJ, Pashley RM (1991) Direct measurement of colloidal forces using an atomic force microscope.Nature 353: 239–241

    CAS  Google Scholar 

  • Edstrom RD, Meinke MH, Yang X, Yang R, Elings V, Evans F (1990) Direct visualization of phosphorylase-phosphorylase kinase complexes by scanning tunneling and atomic force microscopy. Biophys J 58: 1437–1448

    Article  PubMed  CAS  Google Scholar 

  • Emch R, Zenhausern F, Jobin M, Taborelli M, Descouts P (1992) Morphological difference between fibronectin sprayed on mica and on PMMA. Ultramicroscopy 44: 1155–1160

    Article  Google Scholar 

  • Grigg DA, Russell PE, Griffith JE, Lucovsky G (1992a) Tip-sample forces in scanning probe microscopy in air and vacuum. J Vac Sci Technol 10 (4): 680–683

    Article  CAS  Google Scholar 

  • Grigg DA, Russell PE, Griffith JE, Vasile MJ, Fitzgerald EA (1992b) Probe characterization for scanning probe metrology. Ultramicroscopy 44: 1616–1620

    Article  Google Scholar 

  • Hansma HG, Vesenka J, Siegerist C, Kelderman G, Morett H, Sinsheimer RL, Elings V, Bustamante C, Hansma PK (1992) Reproducible imaging and dissection of plasmid DNA under liquid with the atomic force microscope. Science 256: 1180–1184

    Article  PubMed  CAS  Google Scholar 

  • Henderson EH (1992) Imaging and nanodissection of individual supercoiled plasmids by atomic force microscopy. Nucleic Acid Res 20: 445–447

    Article  PubMed  CAS  Google Scholar 

  • Hoh JH, Cleveland JP, Prater CB, Revel JP, Hansma PK (1992) Quantized adhesion detected with the atomic force microscope. J Am Chem Soc 114: 4917–4918

    Article  CAS  Google Scholar 

  • Hoh JH, Lal R, John SA, Revel JP, Arnsdorf MF (1991) Atomic force microscopy and dissection of gap junctions. Science 253: 1405–1408

    Article  PubMed  CAS  Google Scholar 

  • Hoh JH, Revel JP, Hansma PK (1992) Membrane-membrane and membrane-substrate adhesion during dissection of gap junctions with the atomic force microscope. Proc Soc Photo-Opt Instrument Eng 1639: 212–215

    Google Scholar 

  • Keller D, Chih-Chung C (1992) Imaging steep, high structures by scanning force microscopy with electron beam deposited tips. Surf Sci 268–333

    Google Scholar 

  • Lea AS, Pungor A, Hlady V, Andrade JD, Herron JN, Woss EW Jr. (1992) Manipulation of proteins on mica by atomic force microscopy. Langmuir 8: 68–73

    Article  CAS  Google Scholar 

  • Marti Y, Williams CC, Wickramasinghe HK (1987) Atomic force microscope-force mapping and profiling on a sub 100-A scale. J Appl Phys 61: 4723–4729

    Article  Google Scholar 

  • Meyer E, Howard L, Overney RM, Heinzelmann H, Frommer J, Günterherodt HJ, Wagner T, Schier H, Roth S (1991) Molecular-resolution images of Langmuir-Blodgett films using atomic force microscopy. Nature 349: 398–400

    Article  CAS  Google Scholar 

  • Meyer G, Amer NM (1988) Novel optical approach to atomic force microscopy. Appl Phys Lett 53: 1045–1047

    Article  Google Scholar 

  • Nygren H, Stenberg J (1988) Molecular and supramolecular structure of adsorbed fibrinogen and adsorption isotherms of fibrinogen at quartz surfaces. Mater Res 22: 1–11

    Article  CAS  Google Scholar 

  • Ohnesorge F et al. (1992) Scanning force microscopy studies of the S-layers from Bacillus coagulans E38–66, Bacillus sphaericus CCM2177 and of an anbitody binding process. Ultramicroscopy 42: 1236–1242

    Article  PubMed  Google Scholar 

  • Ohinishi S, Hara M, Furuno T, Sasabe H (1992) Imaging the ordered arrays of water-soluble protein ferritin with the atomic force microscope. Biophys J 63: 1425–1431

    Article  Google Scholar 

  • Rugar D, Mamin HJ, Erlandsson R, Stern JE, Terris BD (1988) Force microscope using a fiber-optic displacement sensor. Rev Sci Instr 59: 2337–2340

    Article  CAS  Google Scholar 

  • Thundat T, Allison DP, Warmack RJ, Ferrell TL (1992) Imaging isolated strands of DNA molecules by atomic force microscopy. Ultramicroscopy 44: 1101–1106.

    Article  Google Scholar 

  • Vesenka J, Guthold M, Tang CL, Keller D, Delaine E, Bustamante C (1992) Substrate preparation for reliable imaging of DNA molecules with the scanning force microscope. Ultramicroscopy 44: 1243–1249

    Article  Google Scholar 

  • Weisenhorn A, Drake B, Prater CB, Gould SAC, Hansma PK, Ohnesorge F, Egger M, Heyn SP, Gaub HE (1990a) Immobilized proteins in buffer imaged at molecular resolution by atomic force microscopy. Biophys J 58: 1251–1258

    Article  PubMed  CAS  Google Scholar 

  • Weisenhorn A, Gaub HE, Hansma HG, Sinsheimer RL, Kelderman GL, Hansma PK (1990b) Imaging single-stranded DNA, antibody-antigen reaction and polymerized Langmuir-Blodgett films with an Atomic Force Microscope. Scanning Microscopy 4: 511–516

    PubMed  CAS  Google Scholar 

  • Wigren R, Elwing H, Erlandsson R, Welin S, Lundström I (1991) Structure of adsorbed fibrinogen obtained by scanning force microscopy. FIBS Lett. 280: 225–228

    Article  CAS  Google Scholar 

Download references

Authors
  1. R. Erlandsson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. Olsson
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. INSERM 0387, Laboratoire d’Immunologie, Hopital de Sainte-Marguerite, 13277, Marseille Cedex 09, France

    Pierre Bongrand

  2. The Surface Force Group, Department of Physical Chemistry, The Royal Institute of Technology, S-114 86, Stockholm, Sweden

    Per M. Claesson

  3. Department of Cell Biology, University of Glasgow, G12 8QQ, Glasgow, Scotland, UK

    Adam S. G. Curtis

Rights and permissions

Reprints and permissions

Copyright information

© 1994 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Erlandsson, R., Olsson, L. (1994). The Study of Biomolecules on Surfaces Using the Scanning Force Microscope. In: Bongrand, P., Claesson, P.M., Curtis, A.S.G. (eds) Studying Cell Adhesion. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-03008-0_4

Download citation

  • DOI: https://doi.org/10.1007/978-3-662-03008-0_4

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-03010-3

  • Online ISBN: 978-3-662-03008-0

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation