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Techniques for Studying Blood Platelet Adhesion

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Studying Cell Adhesion
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Abstract

Adhesion of blood platelets to the vessel wall is the first step in the formation of a haemostatic plug or thrombus. Because of this, it is an important and crucial step. Routine techniques for the study of platelet function do not include adhesion studies, partly because no reliable technique is available. Information about adhesive function is sought by indirect means such as aggregation of blood platelets caused by collagen or studies of the von Willebrand factor- Glycoprotein lb interaction via the use of the obsolete antibiotic ristocetin or the snake venom protein botrocetin.

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References

  • Baumgartner HR, Haudenschild C (1972) Adhesion of platelets to subendothelium. Ann N Y Acad Sci 201: 22–36

    Article  PubMed  CAS  Google Scholar 

  • Baumgartner BR, Muggii R (1976a) Adhesion and aggregation: morphologic demonstration, quantitation in vivo and in vitro. In: Gordon JL (ed) Platelets in Biology and Pathology. North Holland, Amsterdam, 23–60

    Google Scholar 

  • Baumgartner HR, Muggii R, Tschopp TB, Turitto VT (1976b) Platelet adhesion, release and aggregation in flowing blood: effects of surface properties and platelet function. Thromb Haemost 35: 124–138

    PubMed  CAS  Google Scholar 

  • Lassila R, Badimon JJ, Vallabhajosula S, Badimon L (1990) Dynamic monitoring of platelet deposition on severely damaged vessel wall in flowing blood. Effects of different stenoses on thrombus growth. Arteriosclerosis 10: 306–315.

    Google Scholar 

  • Muggli R, Baumgartner HR, Tschopp TB, Keller H (1980) Automated microdensitometry and protein assay as a measure for platelet adhesion and aggregation on collagen-coated slides under controlled flow conditions. J Lab Clin Med 95: 195–207

    PubMed  CAS  Google Scholar 

  • Nievelstein PFEM, d’Alessio PA, Sixma JJ (1988) Fibronectin in platelet adhesion to human collagen types I and III. Use of nonfibrillar and fibrillar collagen in flowing blood studies. Arteriosclerosis 8: 200–206

    Google Scholar 

  • Nievelstein PFEM, De Groot PhG, d’Alessio PA, Heijnen HFG, Orlando E, Sixma JJ (1990) Platelet adhesion to vascular cells. The role of exogenous von Willebrand factor in platelet adhesion. Arteriosclerosis 10: 462–469

    Google Scholar 

  • Piotrowicz RS, Orchekowski RP, Nugent DJ, Yamada KY, Kunicki TJ (1988) Glycoprotein Ic-IIa functions as an activation independent fibronectin receptor on human platelets. J Cell Biol 106: 1359–1364

    Article  PubMed  CAS  Google Scholar 

  • Sakariassen KS, Aarts PAMM, De Groot PhG, Houdijk WPM, Sixma JJ (1983) A perfusion chamber developed to investigate platelet interaction in flowing blood with human vessel wall cells, their extracellular matrix and purified components. J Lab Clin Med 102: 522–535

    PubMed  CAS  Google Scholar 

  • Sixma JJ, Nievelstein PE,M, Zwaginga JJ, De Groot PhG (1987) Adhesion of blood platelets to the extracellular matrix of cultured human endothelial cells. Ann N Y Acad Sci 516: 39–51

    CAS  Google Scholar 

  • Staatz WD, Rajpara SM, Wayner EA, Carter WG, Santoro SA (1989) The membrane glycoprotein la-IIa (VLA-2) complex mediates the Mg++-dependent adhesion of platelets to collagen. J Cell Biol 108: 1917–1924

    Article  PubMed  CAS  Google Scholar 

  • Tschopp TB, Baumgartner HR (1975) Physiological experiments in hemostasis and thrombosis. Brit J Haematol 31: 221–229

    Article  Google Scholar 

  • Weiss HJ, Turitto VT, Vicic WJ, Baumgartner HR (1984) Fibrin formation, fibrinopeptide release and platelet thrombus dimension on subendothelium exposed to flowing native blood: greater in factor XII and XI than in factor VIII and IX deficiency. Blood 63: 1004–1015

    PubMed  CAS  Google Scholar 

  • Westphal C, Horler H, Pentz S, Frosch D (1988) A new method for cell culture on an electron-transparent melamine foil suitable for successive LM, TEM and SEM studies of whole cells. J Microsc 150: 225–231

    Google Scholar 

  • Zwaginga JJ, de Boer HC, IJsseldijk MJW, et al (1990a) Thrombogenicity of vascular cells. Comparison between endothelial cells isolated from different sources and smooth muscle cells and fibroblasts. Arteriosclerosis 10: 437–448

    Google Scholar 

  • Zwaginga JJ, IJsseldijk MJW, Beeser-Visser N, De Groot PhG, Vos J, Sixma JJ. (1990b) High von Willebrand factor concentration compensates a relative adhesion defect in uremic blood. Blood 75: 1498–1507

    PubMed  CAS  Google Scholar 

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© 1994 Springer-Verlag Berlin Heidelberg

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Sixma, J.J., de Groot, P.G. (1994). Techniques for Studying Blood Platelet Adhesion. In: Bongrand, P., Claesson, P.M., Curtis, A.S.G. (eds) Studying Cell Adhesion. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-03008-0_12

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  • DOI: https://doi.org/10.1007/978-3-662-03008-0_12

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-03010-3

  • Online ISBN: 978-3-662-03008-0

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