Abstract
Synaptic vesicles are among the best characterized membranous organelles. A rat pheochromocytoma cell line, PC12, makes vesicles of the same size, density and membrane composition as authentic synaptic vesicles, isolated from rat brain (Clift-O’Grady et al., 1990). These organelles have been termed “synaptic vesicle-like vesicles” (SVLVs). In recent years PC12 cells have been used by several investigators as a model system for studying synaptic vesicle biogenesis and the targeting of synaptic vesicle-specific proteins. Evidence has accumulated from these studies that synaptic vesicles can be derived by endocytosis and that the precursor organelle in the nerve terminal is either the plasma membrane or an early endosomal compartment (Johnston et al., 1989; Clift-O’Grady et al., 1990; Regnier-Vigouroux et al., 1991).
The first two authors made equivalent contributions.
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References
Apodaca G, Bomsel M, Arden J, Breitfeld PP, Tang K, Mostov KE (1991) The polymeric immunoglobulin receptor. J Clin Invest 87: 1877–1882
Casanova JE, Breitfeld PP, Ross SA, Mostov KE (1990) Phosphorylation of the polymeric immunoglobulin receptor required for its efficient transcytosis. Science 248: 742–745
Clift-O’Grady L, Linstedt AD, Lowe AW, Grote E, Kelly RB (1990) Biogenesis of synaptic vesicle-like structures in a pheochromocytoma cell line PC-12. J Cell Biol 110: 1693–1703
Johnston PA, Cameron PL, Stukenbrok H, Jahn R, De Camilli P, Suedhof TC (1989) Synaptophysin is targeted to similar microvesicles in CHO and PC12 cells. EMBO J 8: 2863–2872
Regnier-Vigouroux A, Tooze SA, Huttner WB (1991) Newly synthesized synaptophysin is transported to synaptic-like microvesicles via constitutive secretory vesicles and the plasmamembrane. EMBO J 10: 3589–3601
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© 1993 Springer-Verlag Berlin Heidelberg
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Bonzelius, F., Hermani, G.A., Cardone, M.H., Mostov, K.E., Kelly, R.B. (1993). Targeting of the Polymeric Immunoglobulin Receptor in Transfected PC12 Cells. In: Morré, D.J., Howell, K.E., Bergeron, J.J.M. (eds) Molecular Mechanisms of Membrane Traffic. NATO ASI Series, vol 74. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-02928-2_43
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DOI: https://doi.org/10.1007/978-3-662-02928-2_43
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