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Molecular Mechanisms of Membrane Traffic pp 111–112Cite as

Fusicoccin Binding and Internalization by Soybean Protoplasts

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Part of the book series: NATO ASI Series ((ASIH,volume 74))

Abstract

Fusicoccin (FC) is a fungal toxin which binds to a receptor on the plasmalemma of higher plants causing hyperpolarization of the membrane potential and acidification of the apoplastic space (Feyerabend and Weiler 1989). In order to understand the fate of the toxin after its binding, we have developed an FC-containing probe which can be seen by light-or electron microscopy and can be quantified biochemically. The colloidal gold conjugate of bovine serum albumin covalently linked to FC (FC-BSA-Gold) binds to the plasmalemma of intact protoplasts and is partially internalized. Cells treated with FC-BSA-Gold are compared to control cells treated with the gold conjugate of BSA only (BSA-Gold), a marker for fluid-phase uptake. The amount and location of the gold label differ between FC-BSA-Gold and BSA-Gold treated protoplasts. When internalization of FC-BSA-Gold takes place, it may do so, in part, via receptor-mediated endocytosis. The technique described here will allow us to measure and visualize receptor-mediated endocytosis in plant cells.

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References

  • Mersey, B. G., Griffing, L. R., Rennie, P. J. and Fowke, L. C. (1985) The isolation of coated vesicles from protoplasts of soybean. Planta 163: 317–327.

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  • Feyerabend, M. and Weiler, E.W. (1989) Photoaffinity labeling and partial purification of the putative plant receptor for the fungal elicitor toxin, fusicoccin. Planta 178: 282–290

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Author information

Authors and Affiliations

  1. Department of Biology, Texas A & M University, College Station, TX, 77843-3258, USA

    M. A. Villanueva, R. Stout & L. R. Griffing

  2. Department of Biology, Montana State University, Bozeman, MT, USA

    R. Stout

Authors
  1. M. A. Villanueva
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  2. R. Stout
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  3. L. R. Griffing
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Editor information

Editors and Affiliations

  1. Department of Medicinal Chemistry, HANS Life Science Research Building, Purdue University, West Lafayette, IN, USA

    D. James Morré

  2. C & S Biology, School of Medicine University of Colorado, Denver, CO, USA

    Kathryn E. Howell

  3. Department of Anatomy and Cell Biology, McGill University, H3A 2B2, Montreal, PQ, Canada

    John J. M. Bergeron

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© 1993 Springer-Verlag Berlin Heidelberg

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Villanueva, M.A., Stout, R., Griffing, L.R. (1993). Fusicoccin Binding and Internalization by Soybean Protoplasts. In: Morré, D.J., Howell, K.E., Bergeron, J.J.M. (eds) Molecular Mechanisms of Membrane Traffic. NATO ASI Series, vol 74. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-02928-2_24

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  • DOI: https://doi.org/10.1007/978-3-662-02928-2_24

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-02930-5

  • Online ISBN: 978-3-662-02928-2

  • eBook Packages: Springer Book Archive

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