Direct Effect of Phosphatidylserine on the Activity of the Post-Synaptic GABAA Receptor Complex

  • M. V. Rapallino
  • A. Cupello
  • P. Mainardi
  • G. Besio
  • C. W. Loeb
Conference paper
Part of the Nato ASI Series book series (NATO ASI, volume 70)

Abstract

Neurotransmitter postsynaptic receptors, as integral membrane proteins, are located within the phospholipid bilayer of the acceptive membrane. Therefore it appears reasonable that the phospholipid environment of the plasma membrane influences and modulates the functional activity of these receptors. In addition, the phospholipid composition of the two leaflets of the bilayer may introduce a further element able to influence the function of these receptors.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Fujimoto M, Okabayashi T (1983) Influence of phospholipase treatments on ligand binding to a benzodiazepine receptor GABA receptor-chloride ionophore complex. Life Sci 32:2393–2400PubMedCrossRefGoogle Scholar
  2. Giambalvo CT, Rosenberg T (1976) The effect of phospholipases and proteases on the binding of γ-amino butyric acid to junctional complexes of rat cerebellum. Biochim Biophys Acta 436:741–756PubMedCrossRefGoogle Scholar
  3. Hydén H, Lange PW, Larsson S (1980) S-100-glia regulation of GABA transport across the nerve cell membrane. J Neurol Sci 45:303–316PubMedCrossRefGoogle Scholar
  4. Hydén H, Cupello A, Palm A (1986) γ-Amino butyric acid stimulates chloride permeability across microdissected Deiters’ neuronal membrane. Brain Res 379:167–170PubMedCrossRefGoogle Scholar
  5. Hydén H, Cupello A, Rapallino MV (1991) Regulation of inhibition by neuronal GABAA receptors interaction. Royal Society of Arts and Sciences, Goteborg, Biomedicine 1:1–78Google Scholar
  6. Lloyd KG, Davidson L (1979) (3H)GABA binding in brains from Huntington’s chorda patients: altered regulation by phospholipid. Science 205:1147–1149PubMedCrossRefGoogle Scholar
  7. Loeb CW, Benassi E, Besio G, Maffini M, Tanganelli P (1982) Liposome entrapped GABA modifies behavioral and electrographic changes of penicillin-induced epileptic activity. Neurology 32:1234–1238PubMedCrossRefGoogle Scholar
  8. Loeb CW, Benassi E, Besio G, Bo GP, Mainardi P, Scotto PA, Faverio A (1984) Antiepileptic effect of γ-amino-butyric acid and phosphatidyl serine in rats. IRCS Med Sci 12:465Google Scholar
  9. Loeb CW, Besio G, Mainardi P, Scotto PA, Benassi E, Bo GP (1986) Liposome-entrapped 7-amino-butyric acid inhibits isoniazid-induced epileptogenic activity in rats. Epilepsia 27:98–102PubMedCrossRefGoogle Scholar
  10. Loeb CW, Marinari UM, Benassi E, Besio G, Cottalasso D, Cupello A, Maffini M, Mainardi P, Pronzato MA, Scotto PA (1988) Phosphatidyl serine increases in vivo the synaptosomal uptake of exogenous GABA in rats. Exp Neurol 99:440–446PubMedCrossRefGoogle Scholar
  11. Rapallino MV, Cupello A, Hydén H (1988) Direct evidence for the presence of GABAA receptors on the Deiters’neurone plasma membrane cytoplasmic side. Brain Res 462:350–353PubMedCrossRefGoogle Scholar
  12. Rapallino MV, Cupello A, Mainardi P, Besio G, Loeb CW (1990) Effect of phosphatidyl serine on the basal and GABA-activated CT permeation across single nerve membranes from rabbit Deiters’ neurons. Neurochem Res 15:593–596PubMedCrossRefGoogle Scholar
  13. Schofield PR, Darlison MG, Fuijta N, Burt DR, Stephenson FA, Rodriguez H, Rhee LM, Ramachandran J, Reale V, Glencorse TA, Seeburg PH, Barnard EA (1987) Sequence and functional expression of the GABAA receptor shows a ligand-gated receptor super-family. Nature 328:221–227.PubMedCrossRefGoogle Scholar
  14. Toffano G, Aldinio C., Balzano M, Leon A, Savoini G (1981) Regulation of GABA receptor binding to synaptic plasma membrane of rat cerebral cortex: the role of endogenous phospholipids. Brain Res 222:95–102PubMedCrossRefGoogle Scholar
  15. Toffano G, Mazzari S, Zanotti A, Bruni A (1984) Synergistic effect of phosphatidyl serine with γ-amino-butyric-acid in antagonizing the isoniazid-induced convulsions in mice. Neurochem Res 9:1065–1073PubMedCrossRefGoogle Scholar
  16. Ueno E, Kuriyama K (1981) Phospholipids and benzodiazepine recognition sites of brain synaptic membranes. Neuropharmacology 20:1169–1176PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • M. V. Rapallino
    • 1
  • A. Cupello
    • 1
  • P. Mainardi
    • 1
  • G. Besio
    • 1
  • C. W. Loeb
    • 1
  1. 1.Clinica NeurologicaUniversità di GenovaGenovaItaly

Personalised recommendations