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Regulation of Mammalian Fertilization by Gamete Adhesion Molecules

  • Paul M. Wassarman
Part of the Schering Foundation Workshop book series (SCHERING FOUND, volume 4)

Abstract

Summarized here are certain aspects of research performed in this laboratory during the past twelve years or so. The focus is on the current status of our attempts to identify and characterize those molecules associated with the surface of mouse eggs and sperm that regulate species-specific gamete adhesion before and after fusion of gametes. We have made considerable headway with the egg surface (zona pellucida) components and, consequently, this aspect constitutes the bulk of the presentation. However, some attention is paid to sperm surface components involved in adhesion of sperm to eggs. It should be noted that there is evidence to suggest that conclusions drawn from our research on mice apply as well to many other mammals, including human beings.

Keywords

Zona Pellucida Acrosome Reaction Cortical Granule Mouse Sperm Acrosomal Membrane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Abdullah M, Kierszenbaum AL (1989) Identification of rat testis galactosyl receptor using antibodies to liver asialoglycoprotein receptor: Purification and localization on surfaces of spermatogenic cells and sperm. J Cell Biol 108: 367–375PubMedCrossRefGoogle Scholar
  2. Aguas AP, Pinto Da Silva P (1989) Bimodal redistribution of surface trans-membrane glycoproteins during Ca2+-dependent secretion (acrosome reaction) in boar spermatozoa. J Cell Sci 93: 467–479PubMedGoogle Scholar
  3. Bleil JD (1991) Sperm receptors of mammalian eggs. In: Wassarman PM (ed) Elements of mammalian fertilization, Vol 1. CRC Press, Boca Raton, pp 133–151Google Scholar
  4. Bleil JD, Wassarman PM (1980a) Structure and function of the zona pellucida: Identification and characterization of the proteins of the mouse oocyte’ s zona pellucida. Dev Biol 76: 185–203PubMedCrossRefGoogle Scholar
  5. Bleil JD, Wassarman PM (1980b) Synthesis of zona pellucida proteins by denuded and follicle-enclosed mouse oocytes during culture in vitro. Proc Natl Acad Sci 77: 1029–1033PubMedCrossRefGoogle Scholar
  6. Bleil JD, Wassarman PM (1980c) Mammalian sperm-egg interaction: Identification of a glycoprotein in mouse egg zona pellucidae possessing receptor activity for sperm. Cell 20: 873–882PubMedCrossRefGoogle Scholar
  7. Bleil JD, Wassarman PM (1983) Sperm-egg interactions in the mouse: Sequence of events and induction of the acrosome reaction by a zona pellucida glycoprotein. Dev Biol 95: 317–324Google Scholar
  8. Bleil JD, Wassarman PM (1986) Autoradiographic visualization of the mouse egg’s sperm receptor bound to sperm. J Cell Biol 102: 1363–1371PubMedCrossRefGoogle Scholar
  9. Bleil JD, Wassarman PM (1988) Galactose at the nonreducing terminus of O-linked oligosaccharides of mouse egg zona pellucida glycoprotein ZP3 is essential for the glycoprotein’s sperm receptor activity. Proc Natl Acad Sci 85: 6778–6782PubMedCrossRefGoogle Scholar
  10. Bleil JD, Wassarman PM (1990) Identification of a ZP3-binding protein on acrosome-intact mouse sperm by photoaffinity crosslinking. Proc Natl Acad Sci 87: 5563–5567PubMedCrossRefGoogle Scholar
  11. Bleil JD, Beall CF, Wassarman PM (1981) Mammalian sperm-egg interaction: Fertilization of mouse eggs triggers modification of the major zona pellucida glycoprotein, ZP2. Dev Biol 86: 189–197PubMedCrossRefGoogle Scholar
  12. Bleil JD, Greve JM, Wassarman PM (1988) Identification of a secondary sperm receptor in the mouse egg zona pellucida: Role in maintenance of binding of acrosome-reacted sperm to eggs. Dev Biol 128: 376–385PubMedCrossRefGoogle Scholar
  13. Chamberlin ME, Dean J (1990) Human homolog of the mouse sperm receptor. Proc Natl Acad Sci 87: 6014–6018PubMedCrossRefGoogle Scholar
  14. Crowell RL, Lonberg-Holm K (eds) (1986) Virus attachment and entry into cells. American Society for Microbiology, Washington, DC, pp 216Google Scholar
  15. Denny, JB, Blobel, G (1984) 1251-labeled crosslinking reagent that is hydrophilic, photoactivatable, and cleavable through an azo linkage. Proc Natl Acad Sci 81: 5286–5290CrossRefGoogle Scholar
  16. Died J (ed) (1989) The mammalian egg coat: structure and function. Springer Verlag, Berlin Heidelberg New YorkGoogle Scholar
  17. Ducibella T (1991) Cortical granules and the cortical reaction. In: Wassarman PM (ed) Elements of mammalian fertilization, Vol 1. CRC Press, Boca Raton, pp 205–231Google Scholar
  18. Norman HM, Bechtol KB, Wassarman PM (1984) Enzymatic dissection of the functions of the mouse egg’s receptor for sperm. Dev Biol 106: 243–255CrossRefGoogle Scholar
  19. Norman HM, Wassarman PM (1985) 0-linked oligosaccharides of mouse egg ZP3 account for its sperm receptor activity. Cell 41: 313–324CrossRefGoogle Scholar
  20. Greve JM, Wassarman PM (1985) Mouse egg extracellular coat is a matrix of interconnected filaments possessing a structural repeat. J Mol Biol 181: 253–264PubMedCrossRefGoogle Scholar
  21. Greve JM, Salzmann GS, Roller RJ, Wassarman PM (1982) Biosynthesis of the major zona pellucida glycoprotein secreted by oocytes during mammalian oogenesis. Cell 31: 749–759PubMedCrossRefGoogle Scholar
  22. Gwatkin RBL (1977) Fertilization mechanisms in man and mammals. Plenum, New York.CrossRefGoogle Scholar
  23. Jaffee, CL, Lis, H, Sharon N (1980) New cleavable photoreactive heterobifunctional cross-linking reagents for studying membrane organization. Biochemistry 19: 4423–4429CrossRefGoogle Scholar
  24. Jones R, Brown CR, Lancaster RT (1988) Carbohydrate-binding properties of boar sperm proacrosin and assessment of its role in sperm-egg recognition and adhesion during fertilization. Development 102: 781–792Google Scholar
  25. Kinloch RA, Wassarman PM (1989) Profile of a mammalian sperm receptor gene. The New Biologist 1: 232–238PubMedGoogle Scholar
  26. Kinloch RA, Roller RJ, Fimiani CM, Wassarman DA, Wassarman PM (1988) Primary structure of the mouse sperm receptor’s polypeptide chain determined by genomic cloning. Proc Natl Acad Sci 85: 6409–6413PubMedCrossRefGoogle Scholar
  27. Kinloch RA, Roller RJ, Wassarman PM (1990a) Organization and expression of the mouse sperm receptor gene. In: Davidson EH, Ruderman JV, Posakony JW (eds) Developmental biology, Vol 125. Wiley-Liss, New York, pp 9–20Google Scholar
  28. Kinloch RA, Ruiz-Seiler B, Wassarman PM (1990b) Genomic organization and polypeptide primary structure of zona pellucida glycoprotein hZP3, the hamster sperm receptor. Dev Biol 142: 414–421PubMedCrossRefGoogle Scholar
  29. Kinloch RA, Mortillo S, Stewart CL, Wassarman PM (1991) Embryonal carcinoma cells transfected with ZP3 genes differentially glycosylate similar polypeptides and secrete active mouse sperm receptor. J Cell Biol 115: 655–664PubMedCrossRefGoogle Scholar
  30. Kopf GS, Gerton GL (1991) The mammalian sperm acrosome and the acrosome reaction. In: Wassarman PM (ed) Elements of mammalian fertilization, Vol 1. CRC Press, Boca Raton, pp 153–203Google Scholar
  31. Lathrop, WF, Carmichael, EP, Myles, DG, Primakoff, P (1990) cDNA cloning reveals the molecular structure of a sperm surface protein, PH-20, involved in sperm-egg adhesion and the wide distribution of its gene among mammals. J Cell Biol 111: 2939–2949PubMedCrossRefGoogle Scholar
  32. Lira SA, Kinloch RA, Mortillo S, Wassarman PM (1990) An upstream region of the mouse ZP3 gene directs expression of firefly luciferase specifically to growing oocytes in transgenic mice. Proc Natl Acad Sci 87: 7215–7219PubMedCrossRefGoogle Scholar
  33. Lunsford RD, Jenkins NA, Kozak CA, Liang LF, Silan CM, Copeland NG, Dean J (1990) Genomic mapping of murine Zp-2 and Zp-3: two oocyte-specific loci encoding zona pellucida proteins. Genomics 6: 184–187PubMedCrossRefGoogle Scholar
  34. Luria SE, Darnell JE, Baltimore D, Campbell A (1978) General virology. John Wiley & Sons, New York, pp 578Google Scholar
  35. Macek MB, Shur BD (1988) Protein-carbohydrate complementarity in mammalian gamete recognition. Gamete Res 20: 93–109PubMedCrossRefGoogle Scholar
  36. Moller CC, Wassarman PM (1989) Characterization of a proteinase that cleaves zona pellucida glycoprotein ZP2 following activation of mouse eggs. Dev Biol 132: 103–112PubMedCrossRefGoogle Scholar
  37. Moller CC, Bleil JD, Kinloch RA, Wassarman PM (1990) Structural and functional relationships between mouse and hamster zona pellucida glycoproteins. Dev Biol 137: 276–286PubMedCrossRefGoogle Scholar
  38. Mortillo S, Wassarman PM (1991) Differential binding of gold-labeled zona pellucida glycoproteins mZP2 and mZP3 to mouse sperm membrane compartments. Development 113: 141–150PubMedGoogle Scholar
  39. Philpott CC, Ringuette MJ, Dean J (1987) Oocyte-specific expression and developmental regulation of ZP3, the sperm receptor of the mouse zona pellucida. Dev Biol 121: 568–575PubMedCrossRefGoogle Scholar
  40. Ringuette MJ, Sobieski DA, Chamow SM, Dean J (1986) Oocyte-specific gene expression: Molecular characterization of a cDNA coding for ZP3, the sperm receptor of the mouse zona pellucida. Proc Natl Acad Sci 83: 4341–4345PubMedCrossRefGoogle Scholar
  41. Ringuette MJ, Chamberlin ME, Baur AW, Sobieski DA, Dean J (1988) Molecular analysis of cDNA coding for ZP3, a sperm binding protein of the mouse zona pellucida. Dev Biol 127: 287–295PubMedCrossRefGoogle Scholar
  42. Roller RJ, Wassarman PM (1983) Role of asparagine-linked oligosaccharides in secretion of glycoproteins of the mouse egg’s extracullar coat. J. Biol Chem 258: 13243–13249PubMedGoogle Scholar
  43. Roller RJ, Kinloch RA, Hiraoka BY, Li SS-L, Wassarman PM (1989) Gene expression during mammalian oogenesis and early embryogenesis: Quantification of three messenger RNAs abundant in fully-grown mouse oocytes. Development 106: 251–261PubMedGoogle Scholar
  44. Rosiere TK, Wassarman PM (1990) Mapping positions of sperm receptor oligosaccharides on the mouse ZP3 polypeptide. J Cell Biol 111: 488aGoogle Scholar
  45. Salzmann GS, Greve JM, Roller RJ, Wassarman PM (1983) Biosynthesis of the sperm receptor during oogenesis in the mouse. EMBO J 2: 14511456Google Scholar
  46. Schickler M, Lira S, Kinloch R, Wassarman P (1992) A mouse oocyte-specific protein that binds to a region of mZP3 promoter responsible for oocyte-specific mZP3 gene expression. Mol Cell Biol 12: 120–127PubMedGoogle Scholar
  47. Shalgi R, Bleil JD, Wassarman PM (1990) Carbohydrate-mediated sperm-egg interactions in mammals. In: Ben-Rafael Z (ed) Advances in assisted reproductive technologies. Plenum, New York, pp 437–441CrossRefGoogle Scholar
  48. Urch UA (1991) Biochemistry and function of acrosin. In: Wassarman PM (ed) Elements of mammalian fertilization, Vol 1. CRC Press, Boca Raton, pp 233–248Google Scholar
  49. Vazquez MH, Phillips DM, Wassarman PM (1989) Interaction of mouse sperm with purified sperm receptors covalently linked to silica beads. J Cell Sci 92: 713–722PubMedGoogle Scholar
  50. Wassarman PM (1987a) The biology and chemistry of fertilization. Science 235: 553–560PubMedCrossRefGoogle Scholar
  51. Wassarman PM (1987b) Early events in mammalian fertilization. Annu Rev Cell Biol 3: 109–142PubMedCrossRefGoogle Scholar
  52. Wassarman PM (1988a) Zona pellucida glycoproteins. Annu Rev Biochem 57: 415–442PubMedCrossRefGoogle Scholar
  53. Wassarman PM (1988b) Fertilization in mammals. Sci Am 256: 78–84CrossRefGoogle Scholar
  54. Wassarman PM (1989) Role of carbohydrates in receptor-mediated fertilization in mammals. In: Bock G, Harnett S (eds) Carbohydrate recognition in cellular function. John Wiley & Sons, New York, pp 135–155Google Scholar
  55. Wassarman PM (1990) Profile of a mammalian sperm receptor. Development 108: 1–17PubMedGoogle Scholar
  56. Wassarman PM (1991a) Cell surface carbohydrates and mammalian fertilization. In: Fukuda M (ed) Cell surface carbohydrates and cell development. CRC Press, Boca Raton, pp 140–164Google Scholar
  57. Wassarman PM (ed) (199 lb) Elements of mammalian fertilization. Vol I, Basic concepts. Vol II, Practical applications. CRC Press, Boca Raton, Vol 1, pp 310, Vol 2, pp 183Google Scholar
  58. Wassarman PM, Mortillo S (1991) Structure of the mouse egg extracellular coat, the zona pellucida. In: Jeon KW, Friedlander M (eds) International review of cytologysurvey of cell biology, Vol 130. Academic Press, New York, pp 85–110Google Scholar
  59. Wassarman PM, Bleil JD, Florman HM, Greve JM, Roller RJ, Salzmann GS, Samuels FG (1985) The mouse egg’s receptor for sperm: What is it and how does it work? Cold Spring Harbor Symp Quant Biol 50: 11–19PubMedCrossRefGoogle Scholar
  60. Yanagimachi R (1988) Mammalian fertilization. In: Knobil E, Neill JD (eds) The physiology of reproduction, Vol 1. Raven Press, New York, pp 135–185Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • Paul M. Wassarman

There are no affiliations available

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