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Epididymal Proteins and Sperm Maturation

  • Trevor G. Cooper
Part of the Schering Foundation Workshop book series (SCHERING FOUND, volume 4)

Abstract

It is appropriate that a Testis Workshop should have as an accessory appendage a session on the epididymis, for the mere production of sperm cells is insufficient for fertility and a period of extra-gonadal maturation and storage of spermatozoa is necessary. There have been recent advances in several areas of epididymal physiology and biochemistry and I shall address two of them. The main growth area, to which most of this review is devoted, is the identification of protein secretion products of the epididymal epithelium and their localization in the epididymis on epididymal spermatozoa and their putative rôles in fertilization. The epididymis is also responsible for modifying proteins already on the sperm when they enter the epididymis. Demonstrations of changes in the ability of sperm to respond to stimulation upon maturation have also provided clues as to what events occur to them in the epididymis.

Keywords

Acrosome Reaction Sperm Maturation Mouse Sperm Human Epididymis Epididymal Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Anakwe OO, Sharma S, Hoff HB, Hardy DM, Gerton GL (1991) Maturation of guinea pig sperm in the epididymis involves the modification of proacrosin oligosaccharide side chains. Molec Reprod Develop 29: 294–301CrossRefGoogle Scholar
  2. Bayard F, Duguet A, Mazzuca M, Faye JC (1981) Study of a glycoprotein produced by the rat epididymis. In: McKerns KW (ed) Reproductive processes and contraception. Plenum Press, London, New York, pp 393–405Google Scholar
  3. Bleil JD, Wassarman PM (1990) Identification of a ZP3-binding protein on acrosome-inact mouse sperm by photoaffinity crosslinking. Proc Natl Acad Sci 87: 5563–5567PubMedCrossRefGoogle Scholar
  4. Brandt H, Acott TS, Johnson DJ, Hoskins DD (1978) Evidence for an epididymal origin of bovine sperm forward motility protein. Biol Reprod 19: 830835Google Scholar
  5. Brooks DE (1981a) Metabolic activity in the epididymis and its regulation by androgens. Physiol Rev 61: 515–555PubMedGoogle Scholar
  6. Brooks DE (198 lb) Secretion of proteins and glycoproteins by the rat epididymis: regional differences, androgen-dependence, and effects of protease inhibitors, procaine, and tunicamycin. Biol Reprod 25: 1099–1117.Google Scholar
  7. Brooks DE (1982) Purification of rat epididymal proteins “D” and “E”, demonstration of shared immunological determinants, and identification of regional synthesis and secretion. Int J Androl 5: 513–524PubMedCrossRefGoogle Scholar
  8. Brooks DE (1983a) Selective binding of specific rat epididymal secretory proteins to spermatozoa and erythrocytes. Gamete Res 7: 367–376CrossRefGoogle Scholar
  9. Brooks DE (1983b) Effect of androgens on protein synthesis and secretion in various regions of the rat epididymis, as analyzed by two-dimensional gel electrophoresis. Mol Cell Endocrinol 29: 255–270PubMedCrossRefGoogle Scholar
  10. Brooks DE (1985) Characterization of a 22kDa protein with widespread tissue distribution but which is uniquely present in secretions of the testis and epididymis and on the surface of spermatozoa. Biochem Biophys Acta 841: 59–70PubMedCrossRefGoogle Scholar
  11. Brooks DE (1987a) Androgen-regulated epididymal secretory proteins associated with post-testicular sperm development. Ann New York Acad Sci US 513: 179–194CrossRefGoogle Scholar
  12. Brooks DE (1987b) The major androgen-regulated secretory proteins in the rat epididymis bear sequence homology with members of the ct2u-globulin superfamily. Biochem Int 14: 235–240PubMedGoogle Scholar
  13. Brooks DE, Higgins SJ (1980) Characterization and androgen-dependence of proteins associated with luminal fluid and spermatozoa in the rat epididymis. J Reprod Fert 59: 363–375CrossRefGoogle Scholar
  14. Brooks DE, Tiver K (1983) Localization of epididymal secretory proteins on rat spermatozoa. J Reprod Fert 69: 651–657CrossRefGoogle Scholar
  15. Brooks DE, Means AR, Wright EJ, Singh SP, Tiver KK (1986a) Molecular cloning of the cDNA for two major androgen-dependent secretory proteins of 18.5 kilodaltons synthesized by the rat epididymis. J Biol Chem 26: 4956–4961Google Scholar
  16. Brooks DE, Means A, R, Wright EJ, Singh SP, Tiver KK (1986b) Molecular cloning of the cDNA for androgen-dependent sperm-coating glycoproteins secreted by the rat epididymis. Eur J Biochem 161: 13–18Google Scholar
  17. Brown CR, von Glos M, Jones R (1983) Changes in plasma membrane glycoproteins of rat spermatozoa during maturation in the epididymis. J Cell Biol 96: 256–264PubMedCrossRefGoogle Scholar
  18. Bunch DO’D, Saling PM (1991) Generation of a mouse sperm membrane fraction with zona receptor activity. Biol Reprod 44: 672–680CrossRefGoogle Scholar
  19. Byers SW, Dym M, Hewlett IK, Qasba PK (1984a) Alpha-lactalbumin-like proteins in the male reproductive tract. Ann N Y Acad Sci 438: 8–17PubMedCrossRefGoogle Scholar
  20. Byers SW, Qasba PK, Paulson HL (1984b) Immunocytochemical localization of -lactalbumin in the male reproductive tract. Biol Reprod 30: 171–177PubMedCrossRefGoogle Scholar
  21. Cameo MS, Blaquier JA (1976) Androgen-controlled specific proteins in rat epididymis. J Endocr 69: 47–55PubMedCrossRefGoogle Scholar
  22. Cameo MS, Cuasnicú PS, Garben JC, Gonzalez Echeverría F, Kohane A, Piazza A, Piíïeiro L, Blaquier JA (1983) Some studies on secretory epididymal proteins and their possible relation to sperm maturation. In: D’Agata R, Lipsett MB, Polosa P, van der Molen HJ (eds) Recent advances in male reproduction: molecular basis and clinical implications. Raven Press New York, pp 27–35Google Scholar
  23. Cameo MS, Gonzalez Echeverría F, Blaquier JA, Burgos MH (1986) Immunochemical localization of epididymal protein DE on rat spermatozoa: its fate after induced acrosome reaction. Gamete Res 15: 247–257CrossRefGoogle Scholar
  24. Charest NJ, Joseph DC, Wilson EM, French FS (1988) Molecular cloning of complementary deoxyribonicleic acid for an androgen-regulated epididymal protein: sequence homology with metalloproteins. Mol Endocrinol 2: 999–1004PubMedCrossRefGoogle Scholar
  25. Collard MW, Sylvester SR, Tsuruta JK, Griswold MD (1988) Biosynthesis and molecular cloning of sulfated glycoprotein 1 secreted by rat Sertoli cells: sequence similarity with the 70-kilodalton precursor to sulfatide/Gml activator. Biochemistry 27: 4557–4564PubMedCrossRefGoogle Scholar
  26. Collard MW, Griswold MD (1987) Biosynthesis and molecular cloning of sul- fated glycoprotein 2 secreted by rat Sertoli cells. Biochemistry 26: ‘32979–3303Google Scholar
  27. Cooper TG (1986) The epididymis, sperm maturation and fertilisation.CrossRefGoogle Scholar
  28. Cooper TG (1990) Epididymal proteins and their clinical relevance. Andrologia 22 Suppl 1: 155–165Google Scholar
  29. Cuasnicii PS, Gonzalez Echeverría F, Piazza A, Blaquier JA (1984a) Addition of androgens to cultured hamster epididymis increases zona recognition by immature spermatozoa. J Reprod Fert 70: 541–547CrossRefGoogle Scholar
  30. Cuasnicú PS, Gonzalez EcheverrIa F, Piazza AD, Pineiro L, Blaquier JA (1984b) Epididymal proteins that mimic the androgenic effect on zona pellucida recognition by immature hamster spermatozoa. J Reprod Fert 71: 427–431CrossRefGoogle Scholar
  31. Cuasnicti PS, Gonzales Echeverria F, Piazza AD, Cameo MS, Blaquier A (1984c) Antibodies against epididymal glycoproteins block fertilizing ability in rat. J Reprod Fert 72: 467–471CrossRefGoogle Scholar
  32. Dacheux F, Dacheux J-L (1988a) Immunocytochemical localization of antagglutinin in the boar epididymis. Cell Tiss Res 252: 329–337Google Scholar
  33. Dacheux F, Dacheux J-L (1988b) Regulation differentielle de la sécrétion d’antagglutine dans l’épididyme de porc: étude immunocytochemique. Re-prod Nutr Develop 28: 1251–1256CrossRefGoogle Scholar
  34. Dacheux J-L, Paquignon M, Combarnous Y (1983) Head-to-head agglutination of ram and boar epididymal spermatozoa and evidence for an epididymal antagglutinin. J Reprod Fert67: 181–189Google Scholar
  35. DeGeyter CD, Cooper TG, DeGeyter M, Nieschlag E (1989) Effects of bovine mammary alpha-lactalbumin on hyperactivation and sperm-zona pellucida binding of mouse spermatozoa. Gamete Research 24: 415–426CrossRefGoogle Scholar
  36. Eddy EM, Vernon RB, Muller CH, Hahnel AC, Fenderson BA (1985) Immunodissection of sperm surface modifications during epididymal maturation. Am J Anat 174: 225–237PubMedCrossRefGoogle Scholar
  37. Eksittikul T, Chulavatnatol (1986) Increase in fluid viscosity during epididymal transit and the immobilization of rat epididymal spermatozoa. Int J Androl 9: 229–240Google Scholar
  38. El Eltreby MF, Beier HM, Elger W, Mahrous AT, Topert M (1983) Immunocytochemical localization of uteroglobin in the genital tract of male rabbits. Cell Tissue Res 229: 61–73Google Scholar
  39. Ellis DH, Hartman TD, Moore HDM (1985) Maturation and function of the hamster spermatozoon probed with monoclonal antibodies. J Reprod Immunol 7: 299–314PubMedCrossRefGoogle Scholar
  40. Faure J, Ghyselinck NB, Jimenez C, Dufaure JP (1991) Specific distribution of messenger ribonucleic acids for 24-kilodalton proteins in the mouse epididymis a revealed by in situ hybridization: developmental expression and regulation in the adult. Biol Reprod 44: 13–22PubMedCrossRefGoogle Scholar
  41. Faye JC, Duguet L, Mazzuca M, Bayard F (1980) Purification, radioimmunoassay, and immunohistochemical localization of a glycoprotein produced by the rat epididymis. Biol Reprod 23: 423–432PubMedCrossRefGoogle Scholar
  42. Feuchter FA, Vernon RB, Eddy EM (1981) Analysis of the sperm surface with monoclonal antibodies: topographically restricted antigens appearing in the epididymis. Biol Reprod 24: 1099–1110PubMedGoogle Scholar
  43. Feuchter FA, Green MF, Tabet AJ (1987) Maturation antigen of the mouse sperm flagellum: II. Origin from holocrine cells of the distal caput epididymis. Anat Rec 217: 146–152Google Scholar
  44. Fournier-Delpech S, Courot M, Dubois MP (1985) Decreased fertility and motility of spermatozoa from rats immunized with a prealbumin epididymal specific glycoprotein. J Androl 6: 246–250PubMedGoogle Scholar
  45. Fox N, Damjanov I, Knowles BB, Solter D (1982) Teratocarcinoma antigen is secreted by epididymal cells and coupled to maturing sperm. Exp Cell Res 137: 485–486PubMedCrossRefGoogle Scholar
  46. Fox N, Damjanov I, Knowles BB, Solter D (1983) Immunohistochemical localization of the mouse stage-specific embryonic antigen 1 in human tissues and tumors. Cancer Res 43: 669–678PubMedGoogle Scholar
  47. Garberi JC, Kohane AC, Cameo MS, Blaquier JA (1979) Isolation and charac- terization of specific rat epididymal proteins. Mol Cell Endocr 13: 73–82CrossRefGoogle Scholar
  48. Garberi JC, Fontana JD, Blaquier JA (1982) Carbohydrate composition of specific rat epididymal protein. Int J Androl 5: 619–626PubMedCrossRefGoogle Scholar
  49. Garcia C, Regalso F, López de Haro MS, Nieto A (1988) Ultrastructural localization of epididymal secretory proteins associated with the surface of spermatozoa from rabbit cauda epididymis. Histochem J 20: 708–714CrossRefGoogle Scholar
  50. Garrett SH, Garrett JE, Douglass J (1991) In situ histochemical analysis of region-specific gene expression in the adult rat epididymis. Molec Reprod Develop 30: 1–17CrossRefGoogle Scholar
  51. Gaunt SJ (1982) A 28K-dalton cell surface autoantigen of spermatogenesis: characterization using a monoclonal antibody. Develop Biol 89: 92–100PubMedCrossRefGoogle Scholar
  52. Gaunt SJ (1983) Spreading of a sperm surface antigen within the plasma membrane of the egg after fertilization in the rat. J Embryol Exp Morph 75: 259–270PubMedGoogle Scholar
  53. Gaunt SJ, Brown CR, Jones R (1983) Identification of mobile and fixed antigens on the plasma memrbane of rat spermatozoa using monoclonal antibodies. Exp Cell Res 144: 275–284PubMedCrossRefGoogle Scholar
  54. Ghyselinck NB, Jimenez C, Courty Y, Dufaure JP (1989) Androgen-dependent messenger RNA(s) related to secretory proteins in the mouse epididymis. J Reprod Fert 85: 631–639CrossRefGoogle Scholar
  55. Ghyselinck NB, Jimenez C, Lefrancois AM, Dufaure JP (1990) Molecular cloning of a cDNA for androgen-regulated proteins secreted by the mouse epididymis. J Mol Endocrinol 4: 5–12PubMedCrossRefGoogle Scholar
  56. Ghyselink NB, Rigaudiere N, Dufaure J-P (1991) Une protéine androgéno-dépendante sécrétée par la tête de l’épididyme de souris présente des homologies élevées avec différentes glutathion peroxydases. C. R. Acad Sci 313: 1–6Google Scholar
  57. Goldin BF, Voulalas PJ, On GA (1989) Characterization of the maturation-associated galactose oxidase-sensitive glycoproteins of rat caudal sperm plasma membrane and epididymal fluid. Arch Biochem Biophys 270: 208218Google Scholar
  58. Gonzalez Echeverría FM, Cuasnicú PS, Blaquier JA (1982) Identification of androgen-dependent glycoproteins in the hamster epididymis and their association with spermatozoa. J Reprod Fert 64: 1–7CrossRefGoogle Scholar
  59. Gonzalez Echeverría F, Cuasnicú PS, Piazza A, Pineiro L, Blaquier JA (1984) Addition of an androgen-free hamster epididymal protein extract increases the ability of immature hamster spermatozoa to fertilize in vivo and in vitro. J Reprod Fert 71: 432–437Google Scholar
  60. Graham JK, Nolan JP, Hammerstedt RH (1991) Effect of dilauroylphosphatidylcholine liposomes on motility, induction of the acrosome reaction and subsequent egg penetration of ram epididymal sperm. Biol Reprod 44: 1092–1099PubMedCrossRefGoogle Scholar
  61. Hall JC, Hadley J, Doman T (1991) Correlation betwen changes in rat sperm membrane lipids, protein, and the membrane physical state during epididymal maturation. J Androl 12: 76–87PubMedGoogle Scholar
  62. Hamilton DW (1981) Evidence for a-lactalbumin-like activity in reproductive tract fluids of the male rat. Biol Reprod 25: 385–392PubMedCrossRefGoogle Scholar
  63. Hermo L, Wright J, Oko R, Morlaes CR (1991) Role of epithelial cells of the male excurrent duct system of the rat in the endocytosis of secretion of sulfated glycoprotein-2 (clusterin). Biol Reprod 44: 1115–1131CrossRefGoogle Scholar
  64. Hölpert M, Cooper TG (1990a) Improved assays of a-lactalbumin and galactosyltransferase. Analyt Biochem 108: 168–175CrossRefGoogle Scholar
  65. Hölpert M, Cooper TG (1990b) Re-examination of the presence of a-lactalbumin in the rat epididymis. J Reprod Fert 90: 503–514CrossRefGoogle Scholar
  66. Hoos PC, Olson GE (1988) Characterization of a 23kDa sperm-binding protein polypeptide of the golden hamster epididymis. Biol Reprod 39: 131–140PubMedCrossRefGoogle Scholar
  67. Iusem ND, Pineiro L, Blaquier JA, Belocopitow E (1989) Identification of a major secretory protein from rat epididymis: Interaction with spermatozoa. Biol Reprod 40: 307–336Google Scholar
  68. Jimenez C, Ghyselinck NB, Depeiges A, Dufaure J-P (1990) Immunocytochemical localization and association with spermatozoa of androgen-regu-lated proteins of Mr 24 000 secreted by the mouse epididymis. Biol Cell 68: 171–174PubMedCrossRefGoogle Scholar
  69. Jones R (1989) Membrane remodelling during sperm maturation in the epididymis. Oxford Reviews of Reproductive Biology 11: 285–337PubMedGoogle Scholar
  70. Jones R, Brown CR (1982) Association of epididymal secretory proteins. showing -lactalbumin-like activity with the plasma membrane of rat spermatozoa. Biochem J 206: 161–164PubMedGoogle Scholar
  71. Jones R, Brown CR (1987) Identification and characterization of the 2D6 and Mr 23000 antigens on the plasma membrane of rat spermatozoa. Biochem J 241: 353–360PubMedGoogle Scholar
  72. Jones R, von Glos KI, Brown CR (1980a) The synthesis of a sperm-coating protein in the initial segment of the rat epididymis is stimulated by factors in testicular fluid. IRCS Med Sci 8: 56Google Scholar
  73. Jones R, Brown CR, von Glos KI, Parker MG (1980b) Hormonal regulation of proteins synthesis in the rat epididymis. Characterizatiom of androgen-dependent and testicular fluid-dependent proteins. Biochem J 188: 667–676Google Scholar
  74. Jones R, Brown CR, Cran DG, Gaunt SJ (1983) Surface and integral antigens of rat spermatozoa distinguished using monoclonal antibodies. Gamete Res 8: 255–265CrossRefGoogle Scholar
  75. Jones R, Brown CR, von Glos KI, Gaunt SJ (1985) Development of a maturation antigen on the plasma membrane of rat spermatozoa in the epididymis and its fate during fertilization. Exp Cell Res 156: 31–44PubMedCrossRefGoogle Scholar
  76. Kann ML, Serres C (1980) Development and initiation of sperm motility in the hamster epididymis. Reprod Nutr Develop 20: 1739–1749CrossRefGoogle Scholar
  77. Kann ML, Raynaud F (1982) In vivo fertilization after initiation of sperm motility in the hamster epididymis. Reprod Nutr Develop 22: 455–463Google Scholar
  78. Kato M, Weng KS, Kato K, Goodman DS (1985) Immunohistochemical studies on the localization of cellular retinol-binding protein in rat testis and epididymis. Biol Reprod 32: 178–189CrossRefGoogle Scholar
  79. Kirchhoff C, Osterhoff C, Habben I, Ivell R (1990) Cloning and analysis of mRNAs expressed specifically in the human epididymis. Int J Androl 13: 155–167PubMedCrossRefGoogle Scholar
  80. Kirchhoff C, Habben I, Ivell R, Krull N (1991) A major human epididymisspecific cDNA encodes a protein with sequence homolgy to extracellular proteinase inhihbitors. Biol Reprod 45: 350–357PubMedCrossRefGoogle Scholar
  81. Kohane AC, Cameo MS, Püïeiro L, Garberi JC, Blaquier JA (1980a) Distribution and site of production of specific proteins in the rat epididymis. Biol Reprod 23: 181–187PubMedCrossRefGoogle Scholar
  82. Kohane AC, Gonzalez-Echeverría FMC, Piíïeiro L, Blaquier JA (1980b) Interaction of proteins of epididymal origin with spermatozoa. Biol Reprod 23: 737–742PubMedCrossRefGoogle Scholar
  83. Lakoski KA, Carron CP, Cabot CL, Sating PM (1988) Epididymal maturation and the acrosome reaction in mouse sperm: response to zona pellucida develops coincident with modifications of M42 antigen. Biol Reprod 38: 221–233PubMedCrossRefGoogle Scholar
  84. Lakoski K, Williams C, Saling P (1989) Proteins of the acrosomal region in mouse sperm: immunological probes reveal post-testicular modifications. Gamete Research 23: 21–37PubMedCrossRefGoogle Scholar
  85. Lea OA, French FS (1981) Characterization of an acidic glycoprotein secreted by principal cells of the rat epididymis. Biochem Biophys Acta 668: 370376Google Scholar
  86. Lea OA, Petrusz P, French FS (1978) Purification and localization of acidic epididymal glycoprotein (AEG): a sperm coating protein secreted by the rat epididymis. Int J Androl Suppl 2: 592–607CrossRefGoogle Scholar
  87. Leyton L, Saling P (1989) 95kd sperm proteins bind ZP3 and serve as tyrosine kinase substrates in response to zona binding. Cell 57: 1123–1130Google Scholar
  88. Limpaseni T, Chulavatnatol M (1986) A rat epididymal sialoprotein with u-lactal-bumin activity. Biochem Int 13: 41–49PubMedGoogle Scholar
  89. Liu HW, Shang ST, Chao CF, Muller C (1991) The secretion of two sperm maturation-related glycoproteins in BALB/c mouse epididymis. Cell Tiss Res 265: 409–414CrossRefGoogle Scholar
  90. Mattmueller DH, Hinton BT (1991) In vivo secretion and association of clusterm (SGP-2) in luminal fluid with spermatozoa in the rat testis and epididymis. Molec Reprod Develop 30: 62–69CrossRefGoogle Scholar
  91. McLaughlin JD, Shur BD (1987) Binding of caput epididymal mouse sperm to the zona pellucida. Developmental Biol 124: 557–561CrossRefGoogle Scholar
  92. Moore HDM (1980) Localization of specific glycoproteins secreted by the rabbit and hamster epididymis. Biol Reprod 22: 705–718PubMedGoogle Scholar
  93. Moore HDM (1981) Glycoprotein secretions of the epididymis in the rabbit and hamster: localization on epididymal spermatozoa and the effect of specific antibodies on fertilization in vivo. J exp Zool 215: 77–85PubMedCrossRefGoogle Scholar
  94. Moore HDM, Hartmann TD (1986) In-vitro development of the fertilizing ability of hamster epididymal spermatozoa after co-culture with epithelium from the proximal cauda epididymidis. J Reprod Fert 78: 347–352CrossRefGoogle Scholar
  95. Moore HDM, Smith CA (1988) The role of the epididymis during maturation of mammalian spermatozoa in vivo and in vitro. Reprod Nutr Develop 28: 1217–1224CrossRefGoogle Scholar
  96. Moore HDM, Hartman TD, Brown AC, Smith CA, Ellis DH (1985) Expression of sperm antigens during spermatogenesis and maturation detected with monoclonal antibodies. Exp clin immunogenetics 2: 84–96Google Scholar
  97. Moore HDM, Hartmann TD, Smith CA (1986) In-vitro culture of hamster epididymal epithelium and induction of sperm motility. J Reprod Fert 78: 327–336CrossRefGoogle Scholar
  98. Moore HDM, Smith CA, Hartman TD (1987) The role of the epididymal epithelium in the development of mammalian sperm-fertilizing capacity in vivo and in vitro. Ann New York Acad Sci US 513: 195–203CrossRefGoogle Scholar
  99. Moore A, White TW, Ensrud KM, Hamilton DW (1989) The major maturation glycoprotein found on rat cauda epididymal sperm surface is linked to the membrane via phosphatidylinositol. Biochem Biophys Res Commun 160: 460–468PubMedCrossRefGoogle Scholar
  100. Moore A, Hall L, Hamilton DW (1990) An 18-kDa androgen-regulated protein that modifies galactosyltransferase activity is synthesised by the rat caput epididymidis, but has no structural similarity to rat milk alphalactalbumin. Biol Reprod 43: 497–506PubMedCrossRefGoogle Scholar
  101. Murphy GJP, Carroll J (1987) Detection and localization of antigens on the surface of mouse spermatozoa. Antigen synthesis in the epididymis. Biochem J 241: 379–387Google Scholar
  102. Newcomer ME, Ong DE (1990) Purification and crystallization of a retinoic acid-binding protein from rat epididymis. J Biol Chem 265: 12876–12879PubMedGoogle Scholar
  103. Olson GE, Hinton BT (1985) Regional differences in luminal fluid polypeptides of the rat testis and epididymis revealed by two-dimensional gel electrophoresis. J Androl 6: 20–34PubMedGoogle Scholar
  104. Olson GE, Lifsics MR, Winfrey VP, Rifkin JM (1987) Modification of that rat sperm flagellar plasma membrane during maturation in the epididymis. J Androl 8: 129–147PubMedGoogle Scholar
  105. Ong DE, Chytil F (1988) Presence of novel retinoic acid-binding proteins in the lumen of rat epididymis. Arch Biochem Biophys 267: 474–478PubMedCrossRefGoogle Scholar
  106. Orgebin-Crist MC, Fournier-Delpech S (1982) Sperm-egg interactions. Evidence for maturational changes during epididymal transit. J Androl 3: 429433Google Scholar
  107. Orgebin-Crist M, Cornwall GA, Holland MK, Rankin TL, Vreeburg JTM (1990) Characterization and regulation of murine secretory proteins. Hormonal communicating events in the testis. Serono Symposia Publications, Raven Press, New York 70: 277–283Google Scholar
  108. Peterson RN, Hunt WP (1989) Identification, isolation, and properties of a plasma membrane protein involved in the adhesion of boar sperm to the porcine zona pellucida. Gamete Res 23: 103–118PubMedCrossRefGoogle Scholar
  109. Peterson RN, Russell LD, Hunt WP (1984) Evidence for specific binding of uncapacitated boar spermatozoa to porcine zonae pellucidae. J exp Zool 231: 137–147PubMedCrossRefGoogle Scholar
  110. Peterson RN, Henry L, Hunt W, Saxena N, Russell LD (1985) Further characterization of boar sperm plasma membrane proteins with affinity for the porcine zona pellucida. Gamete Res 12: 91–100CrossRefGoogle Scholar
  111. Peterson RN, Hunt WP, Henry LH (1986) Interaction of boar spermatozoa with porcine oocytes: increase in proteins with high affinity for the zona pellucida during epididymal transit. Gamete Res 14: 57–64CrossRefGoogle Scholar
  112. Peterson RN, Campbell P, Hunt WP, Bozzola JJ (1991) Two-dimensional polyacrylamide gel electrophoresis characterization of APz, a sperm protein involved in zona binding in the pig and evidence for its binding to specific zona glycoproteins. Molecular Reprod and Develop 28: 260–271CrossRefGoogle Scholar
  113. Phelps BM, Myles DG (1987) The guinea pig sperm plasma membrane protein, PH-20, reaches the surface via two transport pathways and becomes localized to a domain after an inital uniform distribution. Developmental Biol 123: 63–72CrossRefGoogle Scholar
  114. Phelps BM, Primakoff P, Koppel DE, Low MG, Myles DG (1988) Restricted lateral diffusion of PH-20, a PI-anchored sperm membrane protein. Science 240: 1780–1782PubMedCrossRefGoogle Scholar
  115. Phillips DM, Jones R, Shalgi R (1991) Alterations in distribution of surface and intracellular antigens during epididymal maturation of rat spermatozoa. Molec Reprod Develop 29: 347–356CrossRefGoogle Scholar
  116. Pholpramool C, Lea OA, Burrow PV, Dott HM, Setchell BP (1983) The effects of acidic epididymal glycoprotein ( AEG) and some other proteins on the motility of rat epididymal spermatozoa. Int J Androl 6: 240–248Google Scholar
  117. Porter SB, Fraker LD, Chutil F, Ong DE (1983) Localization of cellular retinol-binding protein in several rat tissues. Proc Natl Acad Sci US 80: 65866590Google Scholar
  118. Porter SB, Ong DE, Chytil F, Orgebin-Crist MC (1985) Localization of cellular retinol-binding protein and cellular retinoic acid binding protein in the rat testis and epididymis. J Androl 6: 197–212PubMedGoogle Scholar
  119. Primakoff P, Hyatt H, Myles DG (1985) A role for the migrating sperm surface antigen PH-20 in guinea pig sperm binding to the egg zona pellucida. J Cell Biol 101: 2239–2244PubMedCrossRefGoogle Scholar
  120. Primakoff P, Hyatt H, Tredick-Kline J (1987) Identification and purification of a sperm surface protein with a potential role in sperm-egg membrane fusion. J Cell Biol 104: 141–149PubMedCrossRefGoogle Scholar
  121. Reynolds AB, Thomas TS, Wilson WL, Oliphant G (1989) Concentration of acrosome stabilising factor ( ASF) in rabbit epididymal fluid and species specificity of anti-ASF antibodies. Biol Reprod 40: 673–680Google Scholar
  122. Rifkin JM, Olson GE (1985) Characterization of maturation-dependent extrin- sic proteins of the rat sperm surface. J Cell Biol 100: 1582–1591PubMedCrossRefGoogle Scholar
  123. Ruiz-Bravo N (1988) Tissue and cell specificity of immobilin biosynthesis. Biol Reprod 39: 901–911PubMedCrossRefGoogle Scholar
  124. Russell LD, Peterson RN, Russell TA, Hunt W (1983) Electrophoretic map of boar sperm plasma membrane polypeptides and localization and fractionation of specific polypeptide subclasses. Biol Reprod 28: 393–413PubMedCrossRefGoogle Scholar
  125. Saling PM (1982) Development of the ability to bind to zonae pellucidae during epididymal maturation: reversible immobilisation of mouse spermatozoa by lanthanum. Biol Reprod 26: 429–436PubMedCrossRefGoogle Scholar
  126. Saling PM, Lakoski KA (1985) Mouse sperm antigens that participate in fertilization II. Inhibition of sperm penetration through the zona pellucida using monoclonal antibodies. Biol Reprod 33: 527–536Google Scholar
  127. Saling PM, Irons G, Waibel R (1985) Mouse sperm antigens that participate in fertilization. I. Inhibition of sperm fusion with the egg plasma membrane using monoclonal antibodies. Biol Reprod 33: 515–526Google Scholar
  128. Saxena NK, Russell LD, Saxena N, Peterson RN (1986a) Immunofluorescence antigen localization on boar sperm plasma membranes - monoclonal-anti-bodies reveal apparent new domains and apparent redistribution of surface antigens during sperm maturation and at ejaculation. Anat Rec 214: 238252Google Scholar
  129. Saxena NK, Saxena N, Hunt W, Peterson RN, Henry L, Russell D (1986b) Increase in the concentration of major boar sperm surface proteins during maturation in the epididymis. J Cell Sci 82: 295–308PubMedGoogle Scholar
  130. Schlegel RA, Hammerstedt R, Cofer GP, Kozarsky K, Freidus D, Williams P (1986) Changes in the organization of the lipid bilayer of the plasma membrane during spermatogenesis and epididymal maturation. Biol Reprod 34: 379–391PubMedCrossRefGoogle Scholar
  131. Scully NF, Shaper JH, Shur BD (1987) Spatial and temporal expresion of cell surface galactosyltransferase during mouse spermatogenesis and epididymal maturation. Develop Biol 124: 111–124PubMedCrossRefGoogle Scholar
  132. Shabinowitz RB, Killian GJ (1987) Two-dimensional electrophresis of proteins in principal cells, spermatozoa and fluid associated with rat epididymis. Biol Reprod 36: 753–768CrossRefGoogle Scholar
  133. Shivaji S, Scheit KH, Bhargava PM (1990) Proteins secreted by the epididymis. In: Proteins of seminal plasma, John Wiley & Sons, New York, pp 51–84Google Scholar
  134. Shur BD, Hall NG (1982a) Sperm surface galactosyltransferase activities during in vitro capacitation. J Cell Biol 95: 567–573PubMedCrossRefGoogle Scholar
  135. Shur BD, Hall NG (1982b) A role for mouse sperm surface galactosyltransfer- ase in sperm binding to the egg zona pellucida. J Cell Biol 95: 574–579PubMedCrossRefGoogle Scholar
  136. Shur BD, Neely CA (1988) Plasma membrane association, purification, and partial characterization of mouse sperm ß1,4-galactosyltransferase. J Biol Chem 263: 17706–17714PubMedGoogle Scholar
  137. Smith CA, Hartman TD, Moore HDM (1986) A determinant of Mr 34000 expressed by hamster epididymal epithelium binds specifically to spermatozoa in co-culture. J Reprod Fert 78: 337–345CrossRefGoogle Scholar
  138. Stephens DT, Acott TS, Hoskins DD (1981) A cautionary note on the determination of forward motility protein activity with bovine epididymal spermatozoa. Biol Reprod 25: 945–949PubMedCrossRefGoogle Scholar
  139. Sylvester SR, Skinner MK, Griswold MD (1984) A sulphated glycoprotein synthesized by Sertoli cells and by epididymal cells is a component of the sperm membrane. Biol Reprod 31: 1087–1801PubMedCrossRefGoogle Scholar
  140. Sylvester SR, Morales C, Oko R, Griswold MD (1989) Sulfated glycoprotein1 ( Saposin precursor) in the reproductive tract of the male rat. Biol Reprod 41: 941–948Google Scholar
  141. Sylvester SR, Morales C, Oko R, Griswold MD (1991) Localization of sulfated glycoprotein-2 (clusterin) on spermatozoa and in the reproductive tract of the male rat. Biol Reprod 45: 195–207PubMedCrossRefGoogle Scholar
  142. Tézon JG, Vazquez MH, Pineiro L, de Larminat MA, Blaquier JA (1985a) Identification of androgen-induced proteins in human epididymis. Biol Re-prod 32: 584–590CrossRefGoogle Scholar
  143. Tézon JG, Ramelia E, Cameo MS, Vazquez MH, Blaquier JA (1985b) Immunocytochemical localization of secretion antigens in the human epididymis and their association with spermatozoa. Biol Reprod 32: 591–597PubMedCrossRefGoogle Scholar
  144. Thomas TS, Reynolds AL, Oliphant G (1984) Evaluation of the site of synthesis of rabbit sperm acrosome stabilizing factor using immunocytochemical and metabolic labeling techniques. Biol Reprod 30: 693–705PubMedCrossRefGoogle Scholar
  145. Thomas TS, Wilson WL, Reynolds AB, Oliphant G (1986) Chemical and physical characterization of rabbit sperm acrosome stabilizing factor. Biol Reprod 35: 691–703PubMedCrossRefGoogle Scholar
  146. Turner TT, Reich GW (1987) Influence of proteins in rat cauda epididymal lumen fluid on cauda sperm motility. Gamete Res 18: 267–278PubMedCrossRefGoogle Scholar
  147. Usselman MC, Cone R (1983) Rat sperm are mechanically immobilized in the caudal epididymis by “immobilin” a high molecular weight glycoprotein. Biol Reprod 29: 1241–1253PubMedCrossRefGoogle Scholar
  148. Usselman MC, Cone RA, Rossignol P (1985) Rat cauda epididymal fluid is a mucus. J Androl 6: 315–320PubMedGoogle Scholar
  149. Vernon RB, Mueller CH, Herr JC, Feuchter FA, Eddy EM (1982) Epididymal secretion of a mouse sperm surface component recognized by a monoclonal antibody. Biol Reprod 26: 523–535PubMedCrossRefGoogle Scholar
  150. Vernon RB, Hamilton MS, Eddy EM (1985) Effects of in vivo and in vitro fertilization environments on the expression of a surface antigen of the mouse sperm tail. Biol Reprod 32: 669–680PubMedCrossRefGoogle Scholar
  151. Vernon RB, Miller CH, Eddy EM (1987) Further characterization of a secreted epididymal glycoprotein in mice that binds to sperm tails. J Androl 8: 123128Google Scholar
  152. Walker JE, Jones R, Moore A, Hamilton DW, Hall L (1990) Analysis of major androgen-regulated cDNA clones from the rat epididymis. Mol cell Endocrinol 74: 61–68PubMedCrossRefGoogle Scholar
  153. Williams RM, Graham JK, Hammerstedt RH (1991) Determination of the capacity of ram epididymal and ejaculated sperm to undergo the acrosome reaction and penetrate ova. Biol Reprod 44: 1080–1091PubMedCrossRefGoogle Scholar
  154. Wilson WL, Reynolds AB, Marta J, Oliphant G (1987) Correlation of conformational changes in the acrosome stabilizing factor ( ASF) with its biological activity. Biol Reprod 36: 1069–1077Google Scholar
  155. Wong PYD, Tsang AYF (1982) Studies on the binding of a 32K rat epididy- mal protein to rat epididymal spermaotoza. Biol Reprod 27: 1239–1246PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • Trevor G. Cooper

There are no affiliations available

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