Abstract
The DNA content of each human chromosome can be measured by flow cytometry, and the results of analyzing a large number of isolated metaphase chromosomes can be accumulated to form a flow karyotype. All the human chromosomes, except for 9–12, can be resolved [1], and small but useful quantities of individual chromosomes can be purified by flow sorting [2]. This material has been used to generate chromosome-specific recombinant DNA libraries which have been used by the scientific communitity as a source of markers linked to genes involved in genetic disease. Recently these libraries have also been used as a source of unique sequences for painting individual chromosomes in metaphase spreads by in situ hybridization [3]. Chromosome sorting has also been used for gene mapping, when cloned DNA probes are localized to a specific chromosome or subregion. This application can involve either filter hybridization [4] or a rapid technique based on the polymerase chain reaction (PCR) [5].
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References
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© 1992 Springer-Verlag Berlin Heidelberg
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Fantes, J.A., Green, D.K. (1992). Fluorescence-Activated Chromosome Sorting. In: Radbruch, A. (eds) Flow Cytometry and Cell Sorting. Springer Laboratory. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-02785-1_19
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DOI: https://doi.org/10.1007/978-3-662-02785-1_19
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