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Molecular Analysis of Transcriptional Control: The FACS-Galactosidase Assay

Part of the Springer Laboratory book series (SLM)

Abstract

The FACS-galactosidase (FACS-Gal) assay measures Escherichia coli lacZ-encoded ′-galactosidase activity in eukaryotic cells. Based on analysis by flow cytometry, the enzyme assay allows the simultaneous use of even a single, stably integrated lacZ gene as a reportergene for functional analysis of transcriptional control elements. Apart from providing good statistics due to the high number of cells analyzed, lacZ expression can be measured quantitatively on a per cell basis. Also, lacZ expression can be correlated with other cellular parameters such as immunophenotype. Cells can be sorted alive according to lacZ expression, from less than 5 to more than 50 000 enzyme molecules per cell, for further cellular and molecular studies.

Keywords

Galactosidase Activity lacZ Expression Cellular Parameter Staining Medium Hydroxyethylpiperazine Ethanesulfonic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • S. Jung

There are no affiliations available

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