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Tissue Culture and Plant Pathology

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Abstract

Plant cell and protoplast cultures provide important tools to clarify many details of host cell-pathogen interactions at the cellular level (see Hildebrandt, 1958; Braun, 1959; Hirth, 1960; Ball, 1966; Bajaj and Bopp, 1971; Takebe and Otsuki, 1974). Single cells of bacterial plant pathogens were isolated and established as clones by Wright et al. (1930). Subsequently, single cell clones of fungi, animal and higher plants have been successfully established. These single cell clones of higher plants were established from isolated single cells and are to be differentiated from strains of cells. Strains of cells may be derived by plating methods on agar and may result from growth of more than a single isolated cell from suspension cultures comprised of mixtures of single cells and small colonies of cells. These latter strains of cells were not necessarily derived from single cells and can, therefore, be differentiated from true single-cell clones. Single-cell clones have provided the means for pure culture studies for higher plant cells comparable to those used for microorganisms. Several methods have been useful to establish single cell clones of higher plant cells.

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Hildebrandt, A.C., Quak, F., Mellor, F.C., Stace-Smith, R. (1977). Tissue Culture and Plant Pathology. In: Reinert, J., Bajaj, Y.P.S. (eds) Applied and Fundamental Aspects of Plant Cell, Tissue, and Organ Culture. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-02279-5_5

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