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Applied and Fundamental Aspects of Plant Cell, Tissue, and Organ Culture pp 465–577Cite as

Protoplasts, Somatic Hybridization and Genetic Engineering

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Abstract

One of the most significant developments in the field of plant tissue culture during. recent years has been the isolation, culture and fusion of protoplasts (Coking, 1972). The techniques are especially important because of their far-reaching implications in studies of plant improvement by cell modification and somatic hybridization (see Bajaj, 1974a). Isolated protoplasts also offer a means of tackling various fundamental and pragmatic research problems in experimental plant biology. This can be realized mainly because of the totipotent nature of plant cells. The literature accumulated so far has revealed that protoplasts in culture can be regenerated into an entire plant; they can be induced to undergo intra- and interspecific fusion to form a somatic hybrid, and also to take up foreign organelles and genetic materials. In addition to general protoplast culture and somatic hybridization studies referred to in this article, protoplasts have been used to investigate various problems in plant physiology (Boulware and Camper, 1972; Bayer, 1973; Hess and Endress, 1973; Kanai and Edwards, 1973; Börner, 1973; Birecka and Miller, 1974; Hall and Cocking, 1974; Hoffmann and Kull, 1974; Gutierrez et al., 1974; Ku et al., 1974; Podbielkowska et al., 1975; Shepard and Totten, 1975), radiobiology (Ohyama et al.,1974; Galun and Raveh, 1975; Howland, 1975; Howland et al., 1975), virology and pathology (Cocking, 1966; Aoki and Takebe, 1969; Hibi and Yora, 1972; Coutts, 1973; Honda et al., 1973; Burgess et al., 1974; Sarkar et al., 1974; Zaitlin and Beachy, 1974; Birecka et al., 1975a, b; Pelcher et al.,1975), cytogenetics (Carlson, 1973 a, Chaleff and Carlson, 1974) and cell modification and uptake studies (Davey and Cocking, 1972; Hess, 1973a, b; Hoffmann, 1973; Hoffmann and Hess, 1973; Blaschek et al., 1974; Giles, 1974; Holl et al., 1974).

Keywords

  • Somatic Hybrid
  • Mesophyll Protoplast
  • Protoplast Isolation
  • Anthocyanin Synthesis
  • Petunia Hybrida

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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  1. Y. P. S. Bajaj
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  2. J. B. Power
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  3. E. C. Cocking
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  4. D. Hess
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Editors and Affiliations

  1. Institut für Pflanzenphysiologie und Zellbiologie, Freie Universität Berlin, 1 Berlin 33, Germany

    Professor Dr Jakob Reinert

  2. A-137 New Friends Colony, 1 10 065, New Delhi, India

    Professor Dr Yashpal P. Singh Bajaj

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Bajaj, Y.P.S., Power, J.B., Cocking, E.C., Hess, D., Giles, K.L., Davey, M.R. (1977). Protoplasts, Somatic Hybridization and Genetic Engineering. In: Reinert, J., Bajaj, Y.P.S. (eds) Applied and Fundamental Aspects of Plant Cell, Tissue, and Organ Culture. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-02279-5_4

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