Abstract
Several sensitive parathyroid hormone (PTH) assays have been published, but differentiation between hypoparathyroid and healthy subjects remains a problem [7, 15, 16, 17, 18, 22, 23]. The main difficulty of the PTH radioimmunoassay (RIA) is the lack of assay sensitivity. This causes hypoparathyroid sera to be mistakenly placed within the normal range or PTH to remain undetectable in normal subjects. We have developed a procedure which is able to differentiate between normal and hypoparathyroid sera [26]. We used the same antiserum as for the “rapid assay” which has been described previously [25]. Guidelines are given for the establishment of highly sensitive assays.
Keywords
- Parathyroid Hormone
- Primary Hyperparathyroidism
- Intact Parathyroid Hormone
- Human Parathyroid Hormone
- Sequential Saturation
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This study was supported by Deutsche Forschungsgemeinschaft (Schm/7–1). Parts of this chapter have been published in Annals of Clinical Biochemistry.
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References
Allgrove J, Chayen J, O’Riordan JLH (1983) The cytochemical bioassay of parathyroid hormone: further experience. J Immunoassay 4:1–19
Armitage EK (1986) Parathyrin (parathyroid hormone): metabolism and methods for assay. Clin Chem 32:418–424
Atkinson MJ, Wong CC, Jippner H (1982) Intact human PTH(h1–84) radioimmunoassay using the h 28–48 peptide. Acta Endocrinol [Suppl] (Copenh) 246:138
Blind E, Schmidt-Gayk H, Scharla S, Flentje D, Fischer S, Göhring U, Hitzler W (1988) Two-site assay of intact parathyroid hormone in the investigation of primary hyperparathyroidism and other disorders of calcium metabolism compared with a midregion assay. J Clin Endocrinol Metab 67:353–360
Brown RC, Aston JP, Weeks I, Woodhead JS (1987) Circulating intact parathyroid hormone measured by a two-site immunochemiluminometric assay. J Clin Endocrinol Metab 65:407–414
Fogh-Andersen N, Ladefoged J, Moller-Petersen J (1984) Renal and hepatic extraction of carboxyl-terminal immunoreactive parathyrin in normal man. J Clin Chem Clin Biochem 22:479–482
Gleed JH, Hendy GN, Nussbaum SR, Rosenblatt M, O’Riordan JLH (1986) Development and application of a mid-region specific assay for human parathyroid hormone. Clin Endocrinol (Oxf) 24:365–373
Goltzman D, Henderson B, Loveridge N (1980) Cytochemical bioassay of parathyroid hormone. Characteristics of the assay and analysis of circulating hormonal forms. J Clin Invest 65:1309–1317
Gorog RH, Hakim MK, Thompson NW, Rigg GA, McCann DS (1982) Radio-immunoassay of serum parathyrin: comparison of five commercial kits. Clin Chem 28:87–91
Hackeng WHL, Lips P, Netelenbos JC, Lips CJM (1986) Clinical implications of estimation of intact parathyroid hormone (PTH) versus total immunoreactive PTH in normal subjects and hyperparathyroid patients. J Clin Endocrinol Metab 63:447–453
Hitzler W, Schmidt-Gayk H, Spiropoulos P, Raue F, Hüfner M (1982) Homologous radioimmunoassay for human parathyrin (residues 53–84). Clin Chem 28:1749–1753
Hunter WM, Greenwood FC (1962) Preparation of iodine-131-labelled human growth hormone of high specific activity. Nature 194:495–496
Klee GG, Preissner CM, Schloegel IW, Kao PC (1988) Bioassay for parathyrin: analytical characteristics and clinical performance in patients with hypercalcemia. Clin Chem 34:482–488
Linda11 AW, Eltring J, Ells J, Roos BA (1983) Estimation of biologically active intact parathyroid hormone in normal and hyperparathyroid sera by sequential N-terminal immunoextraction and midregion radioimmunoassay: J Clin Endocrinol Metab 57:1007–1013
Mallette LE, Tuma SN, Berger RE, Kirkland JL (1982) Radioimmunoassay for the middle region of human parathyroid hormone using an homologous antiserum with a carboxy-terminal fragment of bovine parathyroid hormone as radioligand. J Clin Endocrinol Metab 54:1017–1024
Mallette LE, Wilson DP, Kirkland JL (1983) Evaluation of hypocalcemia with a highly sensitive, homologous radioimmunoassay for the midregion of parathyroid hormone. Pediatrics 71:64–69
Manning RM, Adami S, Papapoulos SE, Gleed JH, Hendy GN, Rosenblatt M, O’Riordan JLH (1981) A carboxy-terminal specific assay for human parathyroid hormone. Clin Endocrinol (Oxf) 15:439–449
Papapoulos SE, Manning RM, Hendy GN, Lewin IG, O’Riordan JLH (1980) Studies of circulating parathyroid hormone in man using a homologous amino-terminal specific immunoradiometric assay. Clin Endocrinol (Oxf) 13:57–67
Schmidt-Gayk H, Schmitt-Fiebig M, Hitzler W, Armbruster FP, Mayer E (1986) Two homologous radioimmunoassays for parathyrin compared and applied to disorders of calcium metabolism. Clin Chem 32:57–62
Schõneshõfer M, Kage A, Kage R, Fenner A (1982) A convenient technique for the specific isolation of I-labelled peptide molecules. Fresenius Z Anal Chem 311:429–430
Slatopolsky E, Martin K, Morrissey J, Hruska K (1982) Current concepts of the metabolism and radioimmunoassay of parathyroid hormone. J Lab Clin Med 99:309–316
Streibl W, Minne H, Raue F, Ziegler R (1979) Radioimmunoassay for human parathyroid hormone for differentiation between patients with hypoparathyroidism, hyperparathyroidism and normals. Horm Metab Res 11:375–376
Wood WG, Butz R, Casaretto M, Hehrmann R, Jippner H, Marschner I, Wachter C, Zahn H, Hesch RD (1980) Preliminary results on the use of an antiserum to human parathyrin in a homologous radioimmunoassay. J Clin Chem Clin Biochem 18:789–795
Zettner A (1973) Principles of competitive binding assays (saturation analyses). I. Equilibrium techniques. Clin Chem 19:669–705
Zillikens D, Armbruster FP, Hitzler W, Horn J, Schmidt-Gayk H (1985) Schneller homologer Radioimmunoassay für human-Parathormon (hPTH) zur Differenzierung der hyperkalzämischen Krise. Ärztl Lab 31:151–156
Zillikens D, Armbruster FP, Stern J, Schmidt-Gayk H, Raue F (1987) Sensitive homologous radioimmunoassay for human parathyroid hormone to diagnose hypo parathyroid conditions. Ann Clin Biochem 24:608–613
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Zillikens, D. (1990). Sensitive Homologous Radioimmunoassay for Human Parathyroid Hormone (Residues 53–84) to Diagnose Hypoparathyroid Conditions: Guidelines for the Establishment of Highly Sensitive Assays. In: Schmidt-Gayk, H., Armbruster, F.P., Bouillon, R. (eds) Calcium Regulating Hormones, Vitamin D Metabolites, and Cyclic AMP Assays and Their Clinical Application. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-00406-7_6
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