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Enhanced Signal Generation by Target Amplification

  • Chapter
Nonradioactive Labeling and Detection of Biomolecules

Part of the book series: Springer Laboratory ((SLM))

Abstract

The polymerase chain reaction (PCR) represents the most common and widespread method for the direct amplification of specific sequences of nucleic acid target molecules. The basic reaction is comprised of three steps:

  1. 1.

    Denaturation of the target DNA

  2. 2.

    Annealing of sequence specific primers

  3. 3.

    Template-specific elongation of these primers with a DNA polymerase and desoxynucleotides

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Authors
  1. Rüdiger Rüger
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  2. David Segev
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  3. Michael C. Little
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  4. James G. Nadeau
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  5. G. Terrance Walker
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  6. James L. Schram
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  7. Melinda S. Fraiser
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  8. Amy Alexander
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  9. Douglas P. Malinowski
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  10. George H. Shimer Jr.
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  11. Keith C. Backman
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  12. E. James
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  13. Erko Stackebrandt
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  14. Werner Liesack
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Editor information

Editors and Affiliations

  1. Forschungszentrum Penzberg, Boehringer Mannheim GmbH, Nonnenwald 2, W-8122, Penzberg, Germany

    Christoph Kessler

  2. Institut für Biochemie, Ludwig-Maximilians-Universität München, Karlstr. 21, W-8000, München 2, Germany

    Christoph Kessler

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© 1992 Springer-Verlag Berlin Heidelberg

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Rüger, R. et al. (1992). Enhanced Signal Generation by Target Amplification. In: Kessler, C. (eds) Nonradioactive Labeling and Detection of Biomolecules. Springer Laboratory. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-00144-8_16

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  • DOI: https://doi.org/10.1007/978-3-662-00144-8_16

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-00146-2

  • Online ISBN: 978-3-662-00144-8

  • eBook Packages: Springer Book Archive

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