Abstract
Fluorescein, resorufin, and rhodamine derivatives are three important fluorescence labels which can be used for labeling of biomolecules. Although there are many more fluorescence labels, which differ in excitation/emission wavelengths, e.g., coumarins, this chapter concentrates mainly on the most popular labels because of their widespread application.
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References
Haugland EP (1982) Covalent fluorescent probes. In: Excited States of Biopolymers. Steiner RF (ed), Plenum Press, New York, London
Hemmilä I (1985) Clinical Chemistry 31: 359
Herrmann R, Josel H-P, Wörner W, Fetterhof TJ (1989) Conjugation of proteins to var- ious fluorescence labels, 19th FEBS meeting, Rom, Abstract Nr FR 511
Nederlof PM, van der Flier S, Wiegant J, Raap AK, Tanke HJ, Ploem JS, van der Ploeg M (1990) Multiple fluorescence in in situ hybridization. Cytometry 11: 126–131
Raffael A (1988) GIT Labor-Medizin, 89–97
Rypacek F, Drobnik J, Kalal J (1980) Fluorescence labeling method for estimation of soluble polymers in living material. Analytical Biochemistry 104: 141–149
Christopoulos TK, Diamandis EP, Wilson G (1991) Quantification of nucleic acids on nitrocellulose membranes with time-resolved fluorometry. Nucleic Acids Res 19: 6015–6019
Diamandis EP (1988) Immunoassays with time-resolved fluorescence spectroscopy. Principles and applications. Clin Biochem 21: 139–150
Diamandis EP (1991) Multiple labeling and time-resolvable fluorophores. Clin Chem 37: 1486–1491
Diamandis EP, Christopoulos TK (1990) Europium chelate labels in time-resolved fluorescence immunoassays and DNA hybridization assays. Anal Chem 62: 1149A - 1157A
Diamandis EP, Christopoulos TK, Bean CC (1992) Quantitative western blot analysis and spot immunodetection using time-resolved fluorometry J Immunol Methods 147: 251–259
Morton RC, Diamandis EP (1990) Streptavidin-based macromolecular complex labeled with a europium chelator suitable for time-resolved fluorescence immunoassay applications. Anal Chem 62: 1841–1845
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning. A laboratory manual. 2nd Edition
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© 1992 Springer-Verlag Berlin Heidelberg
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Josel, HP., Diamandis, E.P., Christopoulos, T.K. (1992). Fluorescence Systems. In: Kessler, C. (eds) Nonradioactive Labeling and Detection of Biomolecules. Springer Laboratory. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-00144-8_14
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DOI: https://doi.org/10.1007/978-3-662-00144-8_14
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-662-00146-2
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