Skip to main content
SpringerLink
Log in

Bestimmung der Xylanase-Aktivität

nach Schinner und von Mersi (1990)

  • Chapter
Bodenbiologische Arbeitsmethoden

  • 120 Accesses

Zusammenfassung

Unter Verwendung von Xylan als Substrat werden Bodenproben 24 Stunden bei 50°C und pH 5,5 inkubiert. Die freigesetzten reduzierenden Zucker bewirken die Reduktion von K-Hexacyanoferrat-III in alkalischer Lösung. Das reduzierte K-Hexacyanoferrat-II reagiert mit Fe-III-Ammo- niumsulfat in saurer Lösung zu einem Fe-III-Hexacyanoferrat-II-Komplex (Berliner Blau), welcher kolorimetrisch erfaßt wird.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 54.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literatur

  • Hashimoto S, Muramatsu T, Fumatsu M (1971) Studies on xylanase from Trichoderma viride. Agr Biol Chem 35:501–508

    Article  CAS  Google Scholar 

  • Kubackova M, Karacsonyi S, Varadi J (1975) Studies on xylanase from Basidiomycetes. Folia Microbiol 20:29–37

    Article  CAS  Google Scholar 

  • Panbangred W, Shiumyo A, Kinoshita S, Okada H (1983) Purification and properties of endoxylanase E.C-3.2.1.8 produced by Bacillus pumilus. Agr Biol Chem 47:957–964

    CAS  Google Scholar 

  • Schinner F, Hofmann J (1978) Cellulase-, Xylanase-und Pectinaseaktivitätsbestimmung in verschiedenen Böden der oberen subalpinen Stufe. In: Cernusca A (ed) Ökologische Analysen von Almflächen im Gasteiner Tal. Universitäts-verlag Wagner, Innsbruck, p 289

    Google Scholar 

  • Schinner F, Mersi von W (1990) Xylanase-, CM-cellulase-and invertase activity in soil, an improved method. Soil Biol Biochem 22:511–515

    Article  CAS  Google Scholar 

  • Sörensen H (1952) On the specifity and products of action of xylanase from Chaetomium globosum. Physiol Plantarum 5:183–198

    Article  Google Scholar 

  • Sörensen H (1955) Xylanase in the soil and the rumen. Nature 176:74

    Google Scholar 

  • Sörensen H (1957) Microbial decomposition of xylan. Acta Agric Scand Suppl 1:1–86

    Google Scholar 

  • Sreenath HK, Joseph R, Murthy VS (1978) Studies on xylan hydrolases from different strains of Streptomyces and their mutual influences in the breakdown of xylan. Folia Microbiol 100:381–393

    Google Scholar 

  • Whistler RL, Masak EJr (1955) Enzymatic hydrolysis of xylan. J Am Chem Soc 77:1241–1243

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Mikrobiologie, Universität Innsbruck, Technikerstraße 25, A-6020, Innsbruck, Austria

    Prof. Dr. Franz Schinner

  2. Bundesanstalt für Agrarbiologie, Wieningerstraße 8, A-4025, Linz, Austria

    Dr. Richard Öhlinger

  3. Bundesanstalt für Bodenwirtschaft, Denisgasse 31-33, A-1200, Wien, Austria

    Dr. Ellen Kandeler

Authors
  1. Prof. Dr. Franz Schinner
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Dr. Richard Öhlinger
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dr. Ellen Kandeler
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

Copyright information

© 1991 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Schinner, F., Öhlinger, R., Kandeler, E. (1991). Bestimmung der Xylanase-Aktivität. In: Bodenbiologische Arbeitsmethoden. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-97284-3_13

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-97284-3_13

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-53143-2

  • Online ISBN: 978-3-642-97284-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation