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The Usefulness of Immunocytological Demonstration of Glial Fibrillary Acidic Protein for Diagnostic Problems of Cerebral Tumours

  • K. H. H. Merkel
  • M. Zimmer
  • W. Wahlen
  • E. Schwarz

Abstract

With the advent of the indirect immunoperoxidase procedure by Nakane und Pierce [13] and the peroxidase-antiperoxidase method by Sternberger et al. [14] we have methods at hand to demonstrate different antibodies in formalin fixed and paraffin embedded tissue.

Keywords

Glial Fibrillary Acidic Protein Glioblastoma Multiforme Giant Cell Tumour Paraffin Embed Tissue Cerebral Tumour 
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References

  1. 1.
    Bignami, A., Eng, L. F., Dahl, D., Uyeda, C. T.: Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence. Brain Res. 43, 429–435 (1972)PubMedCrossRefGoogle Scholar
  2. 2.
    Bignami, A., Dahl, D.: Astrocyte-specific protein and neuroglial differentiation. An immunofluorescent study with antibodies to the glial fibrillary acidic protein. J. Comp. Neurol. 153, 27–38 (1974)PubMedCrossRefGoogle Scholar
  3. 3.
    De Armond, S. J., Eng, L. F., Rubinstein, L. J.: The application of glial fibrillary acidic (GFA) protein immunohistochemistry in neurooncology. Path. Res. Pract. 168, 374–394 (1980)PubMedGoogle Scholar
  4. 4.
    Deck, J. H., Eng, L. F. Bigbee, J., Woodcock, S. M.: The role of glial fibrillary acidic protein in the diagnosis of central nervous system tumours. Acta Neuropathol. (Berl.) 42, 183–190 (1978)CrossRefGoogle Scholar
  5. 5.
    Delpech, B., Delpech, A., Vidard, M. N., Girad, N., Tayot, J., Clement, J. C., Creissard, P.: Glial fibrillary acidic protein in tumours of the nervous system. Brit. J. Cancer 37, 33–40 (1978)PubMedCrossRefGoogle Scholar
  6. 6.
    Duffy, Ph. E., Graf, L., Rappaport, M. M.: Identification of glial fibrillary acidic protein by the immunoperoxidase method in human brain tumours. J. Neuropath. exp. Neurol. 4, 645–651 (1977)CrossRefGoogle Scholar
  7. 7.
    Duffy, Ph. E., Huang Yung-Yu, Rappaport, M. M., Graf, L.: Glial fibrillary acidic protein in giant cell tumours of brain and other gliomas. Acta Neuropath. (Berl.) 52, 51–57 (1980)CrossRefGoogle Scholar
  8. 8.
    Duffy, Ph. E., Graf, L., Huang Yung-Yu, Rappaport, M. M.: Glial fibrillary acidic protein in ependymomas and other brain tumours. J. Neurol. Sci. 40, 133–146 (1979)PubMedCrossRefGoogle Scholar
  9. 9.
    Eng, L. F., Vanderhaegen, J. J., Bignami, A. Gerste, B.: An acidic protein isolated from fibrous astrocytes. Brain Res. 28, 351–354 (1971)PubMedCrossRefGoogle Scholar
  10. 10.
    Eng, L. F., Rubinstein, L. J.: Contribution of immunohistochemistry to diagnostic problems of human cerebral tumours. J. Histochem. Cytochem. 26: 513–522 (1978)PubMedCrossRefGoogle Scholar
  11. 11.
    Goebel, H. H., Schlie, M., Spoerri, O., Eng, L. F.: Die immunhistologische Darstellung des Gliafaserproteins in der neuropathologischen Tumordiagnostik. Verh. dtsch. Ges. Path. 64, 597 (1980)Google Scholar
  12. 12.
    Jacque, C. M., Kujas, M., Poreau, A., Raoul, M., Collier, P., Racadot, J., Baumann, N.: GFA and 5100 protein levels as an index for malignancy in human gliomas and neurinomas. J. Nat. Cancer Inst. 62, 479–483 (1979)PubMedGoogle Scholar
  13. 13.
    Nakane, P. K., Pierce, G. B.: Enzyme-labelled antibodies: Preparation and application for the location of antigen. J. Histochem. Cytochem. 14, 929–931 (1966)PubMedCrossRefGoogle Scholar
  14. 14.
    Sternberger, L. A., Hardy, P. H., Cucutis, J. J., Meyer, H. G.: The unlabelled antibody enzyme method of immunocytochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J. Histochem. Cytochem. 18, 315–333 (1970)PubMedCrossRefGoogle Scholar
  15. 15.
    Uyeda, C. T., Eng, L. F., Bignami, A.: Immunological study of the glial fibrillary acidic protein. Brain Res. 37, 81–89 (1972)PubMedCrossRefGoogle Scholar
  16. 16.
    Van der Meulen, J. D. H., Harthoff, H. J., Ebels, E. J.: Glial fibrillary acidic protein in human gliomas. Neuropath. appl. Neurobiol. 4, 177–190 (1978)CrossRefGoogle Scholar
  17. 17.
    Velasco, M., Dahl, D., Roessmann, U., Gambetti, P.: Immunohistochemical localization of glial fibrillary acidic protein in human glial neoplasms. Cancer 45, 484–494 (1980)PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1982

Authors and Affiliations

  • K. H. H. Merkel
  • M. Zimmer
  • W. Wahlen
  • E. Schwarz

There are no affiliations available

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