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Wachstum

  • Helmut Schraudolf
Part of the Fortschritte der Botanik book series (BOTANY, volume 33)

Zusammenfassung

Nachdem immunologische Methoden mit Erfolg zur quantitativen Bestimmung tierischer Hormone herangezogen worden sind, ist es nicht überraschend, daß nunmehr auch erste Daten zu immunologischen Bestimmungen von Phyto-hormonen vorgelegt wurden (FUCHS u. FUCHS. FUCHS et al.). Tatsächlich werden spezifische Antikörper gegen IES und Gibberellin in Ratten gebildet, die zuvor mit Hämocyaninconjugaten dieser Hormone immunisiert worden waren. Durch Ausarbeitung spezifischer Immunreaktionen, speziell einer Hemmung der Inaktivierung modifizierter T4-Bacteriophagen, gelingt eine Bestimmung beider Hormone. Während die Reaktion auf Gibberelline weitgehend spezifisch ist (S. 130) reagieren im Kreuztest die IES-Sera auch mit Indolacetyl-ε-aminocaprylsäure, Tryptamin und Tryptophan. Eine Vorreinigung IES-haltiger Extrakte ist daher auch für die immunbiologische Bestimmung der Indolauxine notwendig. Die arbeitsintensive Methodik gleicht einstweilen den Vorteil aus, den die immunologische Bestimmung dadurch bietet, daß Wechselwirkungen mit wachsturnsaktiven Verbindungen anderer chemischer Struktur ausgeschlossen sind.

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Copyright information

© Springer-Verlag Berlin · Heidelberg 1971

Authors and Affiliations

  • Helmut Schraudolf
    • 1
  1. 1.Botanisches Institut der UniversitätGießenDeutschland

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