Abstract
Flow microfluorometry is a valuable tool for cell kinetics investi gations [1]. Several mathematical methods are available to evaluate the fraction of cells in cycle phases from a flow-cytometrie (FCM) histogram [2]. A few methods allow to reconstruct the DNA synthesis rate from a histogram measured in balanced exponential growth [3, 4] or from histograms measured during desynchronization [5]. In a recent paper [6], an expression was given to determine the rate of DNA synthesis from a single histogram in balanced growth or from a couple of histograms when the S-phase influx has locally an exponential behaviour. With the aim of evaluating the reliability of the method, in the present paper the rate of DNA synthesis is reconstructed from couples of histograms in saturated stages of growth, obtained by computer simulation.
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Bertuzzi, A., Clerico, R., Gandolfi, A., Germani, A., Vitelli, R. (1985). Determination of DNA Synthesis Rate in Cell Populations with Locally Exponential S-Phase Influx. In: Capasso, V., Grosso, E., Paveri-Fontana, S.L. (eds) Mathematics in Biology and Medicine. Lecture Notes in Biomathematics, vol 57. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-93287-8_35
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DOI: https://doi.org/10.1007/978-3-642-93287-8_35
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