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Automated Analysis of Papanicolaou Stained Cervical Specimens Using a Television-Based Analysis System (Leytas)

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Biomedical Images and Computers

Part of the book series: Lecture Notes in Medical Informatics ((LNMED,volume 17))

Abstract

An image analysis program for automated screening of entire Papanicolaou stained slides, using a television based analysis system (LEYTAS), is described. A series of 45 specimens has been screened to test this program, that consists principally of nuclear contour finding, size and thresholding operations on the nucleus and artefact rejection procedures, using image transformations. The significance of the total number of alarms and the number of detected nuclei per 10.000 epithelial cells as discriminatory factors is discussed with regard to future improvements and developments.

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References

  • ABMAYR, W.; BURGER, G. and SOOST, H. J., Progress report of the TUDAB project for automated cancer cell detection. J. Histochem. Cytochem., 27: 604–612 (1979).

    Article  Google Scholar 

  • AL, I. and PLOEM, J.S., Detection of suspicious cells and rejection of artefacts in cervical cytology using LEYTAS. J. Histochem. Cytochem., 27; 629–634 (1979).

    Article  Google Scholar 

  • BAHR, G.F.; BIBBO,M.; OEHME, M.; PULS, J.H.; REALE, F.R. and WIED, G.L., An automated device for the production of cell preparations suitable for automatic assessment. Acta Cytol., 22: 243–249 (1978).

    Google Scholar 

  • BARRETT, D.L.; JENSEN, R.H.; KING, E.B.; DEAN, P.N. and MAYALL, B., Flow cytometry of human gynecologic specimens using log chromomysin A3 fluorescence and log 90° light scatter. J. Histochem. Cytochem., 27: 573–578 (1979).

    Article  Google Scholar 

  • BARRETT, D.L.; KING, E.B.; JENSEN, R.H. and MERRILL, J.T., Cytomorphology of gynecologic specimens analyzed and sorted by two-parameter flow cytometry. Acta Cytol., 22: 7–14 (1978).

    Google Scholar 

  • CAMBIER, J.L.; KAY, D.B. and WHEELESS, L.L., A multidimensional slit-scan flow system. J. Histochem. Cytochem., 27: 321–324 (1979).

    Article  Google Scholar 

  • CASTLEMAN, K.R. and WHITE, B.S., Optimizing cervical cell classifiers. Analyt. and Quant. Cytol. In Press.

    Google Scholar 

  • DRIEL-KULKER, A.M.J. van; MEYER, F. and PLOEM, J.S., Automated analysis of cervical specimens using the TAS. Acta Microscopica, Suppl. 4. Quantitative Image Analysis and Microphotometry. S. Hirzel Verlag Stuttgart (1980).

    Google Scholar 

  • DRIEL-KULKER, A.M.J. van; PLOEM-ZAAIJER, J.J.; ZWAN-VAN Der Zwan, M. van der and TANKE, H.J., A preparation technique for exfoliated and aspirated cells allowing different staining procedures. Proceedings of Third Intern. Conf. on Automation of Diagnostic Cytology and Cell Image Analysis. Analyt. and Quant. Cytol. In Press

    Google Scholar 

  • EASON, P.J. and TUCKER, J.H., The preparation of cervical scrape material for automated cytology using gallocyanin chrome-alum stain. J. Histochem. Cytochem., 27: 25–31 (1979).

    Article  Google Scholar 

  • GALBRAITH, W.; MARSHALL, P.N.; LEE, E.S. and BACUS, J.W., Studies on Papanicolaou staining: I. visible light spectra of stained cervical cells. Analyt. and Quant. Cytol., 1: 160–168 (1979).

    Google Scholar 

  • HÖFFKEN, H.; OTTO, K. and SOOST, H.J., Cytomorphologic results of preparation experiments for monolayer deposition of cervical material. J. Histochem. Cytochem., 27: 19–24 (1979).

    Article  Google Scholar 

  • HUSAIN, O.A.N.; PAGE ROBERTS, B.A. and MILLET, J.A., A sample preparation for automated cervical cancer screening. Acta Cytol., 22: 15–21 (1978).

    Google Scholar 

  • LEIF, R.C.; GALL, S.; DUNLAP, L.A.; RAILEY, C.; ZUCKER, R.M. and LEIF, S.B., Centrifugal cytology in the preparation of fixed stained dispersions of gynecological cells. Acta Cytol., 19, 159–168 (1975).

    Google Scholar 

  • LEIF, R.C.; NORDQVIST, S.; CLAY, S.; CAYER, M.; INGRAM, D.; CAMERON, B.F.; BOBBIT, D.; GADDIS, R.; LEIF, S.B. and CABANAS, A., A procedure for dissociating ayre scrape samples. J. Histochem. Cytochem., 25: 525–537 (1977).

    Article  Google Scholar 

  • MEYER, F., Interactive image transformations for an automatic screening of cervical smears. J. Histochem. Cytochem., 27: 128–135 (1979).

    Article  Google Scholar 

  • MEYER, F. and DRIEL-KULKER, A.M.J. van, Automatic screening of Papanicolaou stained cervical smears with the TAS. Acta Microscopica. Suppl. 4. Quantitative Image Analysis and Microphotometry. S. Hirzel Verlag, Stuttgart (1980).

    Google Scholar 

  • OTTO, K.; HÖFFKEN, H. and SOOST, H.J., Sedimentation velocity separation: A prepa- ration method for cervical samples. J. Histochem. Cytochem., 27: 14–18 (1979).

    Article  Google Scholar 

  • PLOEM, J.S.; VERWOERD, N.; BONNET, J. and KOPER, G., An automated microscope for quantitative cytology combining television image analysis and stage scanning microphotometry. J. Histochem. Cytochem., 27: 136–143 (1979).

    Article  Google Scholar 

  • READ, J.S.; BOROVEC, R.T.; BARTELS, P.H.; BIBBO, M.; PULS, J.H.; REALE, V.R.; TAYLOR, J. and WIED, G.L., A fast image processor for locating cell nuclei in uterine specimens. Proceedings of the Second Internat. Conf. on the Automation of Cancer Cytology and Cell Image Analysis. Eds. N.J. Pressman and G.L. Wied (1979).

    Google Scholar 

  • ROSENTHAL, D.L.; STERN, E.; LATCHIE, C.Mc.; WU, A.; LABASSE, L.D.; WALL, R. and CASTLEMAN, K.R., A simple method of producing a monolayer of cervical cells for digital image processing. Analyt. and Quant. Cytol., 1: 84 (1979).

    Google Scholar 

  • SERRA, J., Lectures on image analysis by mathematical morphology. Fontainebleau, France.

    Google Scholar 

  • SMEULDERS, A.M.J. ; LEYTE-VELDSTRA, L. ; DRIEL-KULKER, A.M.J. van ; PLOEM, J.S. and CORNELISSE, C.J., Towards real time cell recognition of breast and cervical samples of LEYTAS preselected events. Submitted for publication. Analyt. and Quant. Cytol.

    Google Scholar 

  • StÖHR, M. and GOERTTLER, K., The Heidelberg flow analyzer and sorter (HEIFAS) approach on the prescreening of uterine cancer. J. Histochem. Cytochem., 27: 564–566 (1979).

    Article  Google Scholar 

  • TANAKA, N.; IKEDA, H.; Ueno, T.; MUKAWA, A. and KAMITSUMA, K., Field test and experimental use of CYBEST model 2 for practical gynecologic mass screening. Analyt. and Quant. Cytol., 1: 122–126 (1979).

    Google Scholar 

  • TANKE, H.J.; INGEN, E.M. van and PLOEM, J.S., Acriflavine-Feulgen stilbene (AFS) staining: a procedure for automated cervical cytology with a television based system (LEYTAS). J. Histochem. Cytochem., 27: 84–86 (1979).

    Article  Google Scholar 

  • TUCKER, J.H., An image analysis system for cervical cytology automation using nuclear DNA content. J. Histochem. Cytochem., 27: 613–620 (1979).

    Article  Google Scholar 

  • VROLIJK, J.; PEARSON, P.L. and PLOEM, J.S., Leytas, a system for the processing of microscopic images. Analyt. and Quant. Cytol., 2: 41–48 (1980)

    Google Scholar 

  • WOLLEY, R.C.; HERZ, F.; DEMBITZER, H.M.; SCHREIBER, K. and KOSS, L.G., The monodisperse cervical smear. Analyt. and Quant. Cytol., 1: 43–49 (1979)

    Google Scholar 

  • ZAHNISER, D.J.; OUD, P.S.; RAAIJMAKERS, M.C.T.; VOOYS B.P. and WALLE, R.T. van de, BIOPEPR: A system for the automatic prescreening of cervical smears. J. Histochem. Cytochem., 27: 635–641 (1979).

    Article  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Histochemistry and Cytochemistry, Sylvius Laboratories, University Medical Center, P.O. Box 772, Leiden, The Netherlands

    Johan S. Ploem & Anneke M. J. Van Driel-Kulker

Authors
  1. Johan S. Ploem
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  2. Anneke M. J. Van Driel-Kulker
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Editor information

Editors and Affiliations

  1. School of Engineering, University of California, 92717, Irvine, California, USA

    Jack Sklansky (Seminar Co-Chairmen) (Seminar Co-Chairmen)

  2. Laboratoire de Neurobiologie Quantitative, U.E.R. Biomédicale, 74, rue Marcel Cachin, 93012, Bobigny Cedex, France

    Jean-Claude Bisconte (Seminar Co-Chairmen) (Seminar Co-Chairmen)

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© 1982 Springer-Verlag Berlin Heidelberg

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Ploem, J.S., Van Driel-Kulker, A.M.J. (1982). Automated Analysis of Papanicolaou Stained Cervical Specimens Using a Television-Based Analysis System (Leytas). In: Sklansky, J., Bisconte, JC. (eds) Biomedical Images and Computers. Lecture Notes in Medical Informatics, vol 17. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-93218-2_2

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  • DOI: https://doi.org/10.1007/978-3-642-93218-2_2

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-11579-3

  • Online ISBN: 978-3-642-93218-2

  • eBook Packages: Springer Book Archive

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