Clonal Dominance: Presentation of Antigenic Subsites is the Determining Factor
The difficulty in comprehending the immune system resides in the finding that standard immune responses are — at the level of the bone marrow derived B lymphocyte where antibody synthesis and secretion occur — the sum of many clonal responses, both specific and cross-reactive. Therefore, any study concerned with the expression of clones as well as with its products requires the separation of clones. This aim may be approached in several ways: a) the generation of antibody responses with restricted heterogeneity (1), b) the transfer of limited cell numbers from suitably primed animals into syngeneic X-radiated hosts (2), and c) the production of hybridomas (3). Finally, poly-clonal activation of bone marrow derived cells by mitogens may also be used in systems working with limiting cell numbers to evaluate the frequency of monoclonal precursor cells by their products (4).
KeywordsAntibody Response Affinity Antibody Antibody Population High Affinity Antibody Myeloma Protein
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