Summary
It is suggested that hemolysis is the first step in the degradation of red blood cells after they have entered the phagocytes.
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1.
A method for quantitative determination of the hemolytic activity is described.
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2.
In the homogenate, the hemolytic splenic factor is found to be a structure-bound lipid.
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3.
Quantitatively, the great majority of the hemolytic activity is found in a fraction a considerable part of which are long chain fatty acids but not lysolecithin.
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4.
The hemolytic activity is inhibited by albumin. The pH optimum for hemolytic activity is 4.8–6.0, corresponding to that of lysosomal enzymes. It is suggested that this indicates a regulatory mechanisms which may act in vivo.
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Literatur
Folch, J., Lees, M., Sloane Stanley, G. H.: A simple method for the isolation and purification of total lipides from animal tissues. J. biol. Chem. 226, 497 (1957).
Laser, H.: The isolation of a hemolytic substance from animal tissues and its biological properties. J. Physiol. (Lond.) 110, 338 (1950).
Matsuraba, H., Sasaki, R., Singer, A., Jukes, T. H.: Specific nature of hydrolysis of insulin and tobacco mosaic virus protein by thermolysin. Arch. Biochem. Biophysics 115, 324 (1966).
Nomoto, M., Narahashi, Y., Murakami, M.: Substrate specificity of streptomyces griseus protease. J. Biochem. 48, 906 (1960).
Ponder, E., Ponder, R. V.: Chromatographic identification of lysins in normal tissues and tumours of mice. Nature (Lond.) 204, 995 (1964).
Reinauer, H., Brügelmann, J., Kurz, W., Hollmann, S.: Zur subcellulären Lokalisation der Phospholipasen in der Milz der Ratte. Hoppe-Seyler’s Z. physiol. Chem. 349, 1191 (1968).
SzÄsz, J., Szelenyi, J. G., Gardos, G., Hollän, S. R.: Effect of different washing solutions on some characteristics of erythrocytes. Haematologia 1, 207 (1967).
Tyler, D. B.: The hemolytic and antihemolytic activities of various centrifugally separated fractions of adult and fetal liver cells. Science 112, 456 (1950).
Wagner, H., Hörhammer, L., Wolff, P.: Dünnschichtchromatographie von Phosphatiden und Glykolipiden. Biochem. Z. 334, 175 (1961).
Zöllner, N., Wolfram, G.: Dünnschichtchromatographische Systeme zur Trennung der Plasmalipoide. Klin. Wschr. 40, 1101 (1962).
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© 1970 Springer-Verlag Berlin · Heidelberg
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Schiebel, W., Ruhenstroth-Bauer, G. (1970). Zur Charakterisierung eines hämolysierenden Faktors aus Rattenmilz. In: Lennert, K., Harms, D. (eds) Die Milz / The Spleen. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-92998-4_30
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DOI: https://doi.org/10.1007/978-3-642-92998-4_30
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