Erythrocyte Shape Alteration « In Vitro » : Relationship to Plasma and Cellular Factors Abstract
Erythrocytes in plasma at 37° C change from disc to sphere when ATP falls to <0.25 μmoles/ml RBC at ~16 hours. From 0 to 16 hours, intracellular calcium was zero but rose to 0.11 and 0.51 × 10-17 moles/ cell at 24 and 48 hours. Membrane calcium (0.14 × 10-17 moles/cell) remained unchanged over 48 hours. Erythrocytes in autologous plasma at 37° C were nearly all (>85 %) crenated spheres after 18 hours (ATP <0.15 μmoles/ml) and were tightly crenated or smooth spheres after 36 hours (ATP <0.08 moles/ml). However, erythrocytes were largely (>75 %) discs at 18 hours and many were discoidal from 18 to 36 hours if examined in fresh plasma indicating a plasma factor in spherogenicity although injury does become irreversible.
KeywordsDisc Shape LCAT Activity Autologous Plasma Fresh Plasma Smooth Sphere