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Erythrocyte Shape Alteration « In Vitro » : Relationship to Plasma and Cellular Factors Abstract

  • M. A. Lichtman
  • G. V. Marinetti
Conference paper

Abstract

Erythrocytes in plasma at 37° C change from disc to sphere when ATP falls to <0.25 μmoles/ml RBC at ~16 hours. From 0 to 16 hours, intracellular calcium was zero but rose to 0.11 and 0.51 × 10-17 moles/ cell at 24 and 48 hours. Membrane calcium (0.14 × 10-17 moles/cell) remained unchanged over 48 hours. Erythrocytes in autologous plasma at 37° C were nearly all (>85 %) crenated spheres after 18 hours (ATP <0.15 μmoles/ml) and were tightly crenated or smooth spheres after 36 hours (ATP <0.08 moles/ml). However, erythrocytes were largely (>75 %) discs at 18 hours and many were discoidal from 18 to 36 hours if examined in fresh plasma indicating a plasma factor in spherogenicity although injury does become irreversible.

Keywords

Disc Shape LCAT Activity Autologous Plasma Fresh Plasma Smooth Sphere 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Bessis (M.) and Brecher (G.) (1971): Action du plasma sur la forme des globules rouges. Nouv. Rev. Fr. Hématol., 11, 305.PubMedGoogle Scholar
  2. 2.
    Weed (R. I.), La Celle (P. L.) and Merrill (E. W.) (1969): Metabolic dependence of red cell deformability. J. Clin. Invest., 48, 795.PubMedCrossRefGoogle Scholar

Copyright information

© Masson & Cie, Editeurs, Paris 1973

Authors and Affiliations

  • M. A. Lichtman
    • 1
  • G. V. Marinetti
    • 1
  1. 1.School of Medicine and DentistryUniversity of RochesterRochesterUSA

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