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Peroxisomes pp 81-90 | Cite as

Synthesis of Oligonucleotides Used as Probes; Purification by HPLC

  • M. Bentéjac
  • M. Bugaut
Part of the Springer Laboratory book series (SLM)

Abstract

Synthetic oligonucleotides are useful research tools in molecular biology [3, 7]. As hybridization probes, they can distinguish a perfect match in the target sequences from various single-base-pair mismatches [20, 21]. They have been used to introduce mutations in DNA at specific sites [8, 13, 15, 22, 23] as well as to probe for mutations at specific loci in genes [4, 9, 13, 20, 22, 23]. They have also been employed in the isolation of specific DNA sequences from a bank of cloned cDNA [6, 16, 19, 21] and in the determination of RNA secondary structure [11]. Moreover, the use of antisense DNA or RNA oligonucleotides to block the translation or processing of specific mRNAs provides a powerful tool for studies of the regulation of gene expression and development [2, 5].

Keywords

Synthetic Oligonucleotide Registered Trademark Main Menu OD260 Unit HPLC Purification 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • M. Bentéjac
  • M. Bugaut

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