Peroxisomes pp 37-57 | Cite as

Morphology of Peroxisomes in Light- and Electron Microscopy

  • E. Baumgart
Part of the Springer Laboratory book series (SLM)


Peroxisomes (POs) vary markedly in distribution, morphological shape and enzyme composition in different organs and cell types (for a review see [14]). In the original studies of DE DUVE and associates (for a review see [9]), POs were considered to be present only in a few cell types, like hepatocytes and renal tubular epithelial cells. The development of the alkaline 3,3′-diaminobenzidine (DAB) method for the light- and electron microscopical visualization of the peroxidatic activity of catalase (a marker enzyme of POs)[10, 11] led to the discovery of the ubiquity of this cell organelle [18]. Since POs are involved in the metabolism of long-chain fatty acids, they are very numerous and large in organs metabolizing fatty acids like the liver. Hepatic POs are spherical cell organelles with a single limiting membrane surrounding a homogeneous, finely granular matrix. In some mammalian species (like beef, sheep, cat, rodents), hepatic, or even renal, POs may contain crystalline inclusions in their matrix (cores = crystallized urate oxidase [1, 25]; marginal plates = crystallized α-hydroxy acid oxidase B [29, 30].


Automatic Image Analysis Urate Oxidase Peroxisomal Enzyme Cytochemical Localization Gradient Medium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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© Springer-Verlag Berlin Heidelberg 1994

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  • E. Baumgart

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