Advertisement

Peroxisomes pp 37-57 | Cite as

Morphology of Peroxisomes in Light- and Electron Microscopy

  • E. Baumgart
Chapter
Part of the Springer Laboratory book series (SLM)

Abstract

Peroxisomes (POs) vary markedly in distribution, morphological shape and enzyme composition in different organs and cell types (for a review see [14]). In the original studies of DE DUVE and associates (for a review see [9]), POs were considered to be present only in a few cell types, like hepatocytes and renal tubular epithelial cells. The development of the alkaline 3,3′-diaminobenzidine (DAB) method for the light- and electron microscopical visualization of the peroxidatic activity of catalase (a marker enzyme of POs)[10, 11] led to the discovery of the ubiquity of this cell organelle [18]. Since POs are involved in the metabolism of long-chain fatty acids, they are very numerous and large in organs metabolizing fatty acids like the liver. Hepatic POs are spherical cell organelles with a single limiting membrane surrounding a homogeneous, finely granular matrix. In some mammalian species (like beef, sheep, cat, rodents), hepatic, or even renal, POs may contain crystalline inclusions in their matrix (cores = crystallized urate oxidase [1, 25]; marginal plates = crystallized α-hydroxy acid oxidase B [29, 30].

Keywords

Automatic Image Analysis Urate Oxidase Peroxisomal Enzyme Cytochemical Localization Gradient Medium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Angermüller S, Fahimi HD (1986) Ultrastructural cytochemical localization of uricase in peroxisomes of rat liver. J Histochem Cytochem 34:159–165PubMedCrossRefGoogle Scholar
  2. 2.
    Baudhuin P, Evrard P, Berthet J (1967) Electron microscopic examination of subcellular fractions. I. The preparation of representative samples from suspensions of particles. J Cell Biol 32:181–191PubMedCrossRefGoogle Scholar
  3. 3.
    Baumgart E, Stegmeier K, Schmidt FH, Fahimi HD (1987) Proliferation of peroxisomes in pericentral hepatocytes of rat liver after administration of a new hypochoiesterolemic agent (BM 15766). Sex-dependent ultrastructural differences. Lab Invest 56:554–564PubMedGoogle Scholar
  4. 4.
    Baumgart E, Völkl A, Hashimoto T, Fahimi HD (1989) Biogenesis of peroxisomes: Immunocytochemical investigation of peroxisomal membrane proteins in proliferating rat liver peroxisomes and in catalase-negative membrane loops. J Cell Biol 108:2221–2231PubMedCrossRefGoogle Scholar
  5. 5.
    Baumgart E, Völkl A, Fahimi HD (1990) Proliferation of peroxisomes without simultaneous induction of the peroxisomal fatty acid β-oxidation. FEB S Lett 264: 5–9CrossRefGoogle Scholar
  6. 6.
    Beier K (1992) Light microscopic morphometric analysis of peroxisomes by automatic image analysis: advantages of immunostaining over the alkaline DAB method. J Histochem Cytochem 40:115–121PubMedCrossRefGoogle Scholar
  7. 7.
    Beier K, Fahimi HD (1992) Application of automatic image analysis for quantitative morphological studies of peroxisomes in rat liver in conjunction with cytochemical staining with 3,3′-diaminobenzidine and immunochemistry. Micr Res Tech 21:271–282CrossRefGoogle Scholar
  8. 8.
    Beier K, Völkl A, Hashimoto T, Fahimi HD (1988) Selective induction of peroxisomal enzymes by the hypolipidemic drug bezafibrate. Detection of modulations by automatic image analysis in conjunction with immunoelectron microscopy and immunoblotting. Eur J Cell Biol 46:383–393PubMedGoogle Scholar
  9. 9.
    De Duve C, Baudhuin P (1966) Peroxisomes (microbodies and related particles). Physiol Rev 46:323–357PubMedGoogle Scholar
  10. 10.
    Fahimi HD (1968) Cytochemical localization of peroxidase activity in the rat hepatic microbodies (peroxisomes). J Histochem Cytochem 16:547–550PubMedCrossRefGoogle Scholar
  11. 11.
    Fahimi HD (1969) Cytochemical localization of catalase in rat hepatic microbodies (peroxisomes). J Cell Biol 43:275–288PubMedCrossRefGoogle Scholar
  12. 12.
    Fahimi HD, Baumgart E (1992 in press) Peroxisomes. In: Ogawa K and Barka T (eds) Electron Microscopic Cytochemistry and Immunocytochemistry in Bio medicine. Boca Raton: CRC PressGoogle Scholar
  13. 13.
    Fahimi HD, Reinicke A, Sujatta M, Yokota S, Oezel M, Hartig F, Stegmeier K (1982) The short- and long-term effects of bezafibrate in the rats. Ann NY Acad Sei 386:111–135CrossRefGoogle Scholar
  14. 14.
    Fahimi HD, Sies H (1987) Peroxisomes in Biology and Medicine. Heidelberg: Springer-Verlag, pp. 1–458.CrossRefGoogle Scholar
  15. 15.
    Fringes B, Reith A (1982) Time course of peroxisome biogenesis during adaptation to mild hyperthyroidism in rat liver. A morphometric/stereologic study by electron microscopy. Lab Invest 47:19–26PubMedGoogle Scholar
  16. 16.
    Gorgas K, Krisans S (1989) Zonal heterogeneity of peroxisome proliferation and morphology in rat liver after gemfibrozil treatment. J Lipid Res 30:1859–1875PubMedGoogle Scholar
  17. 17.
    Hartl F-U, Just WW (1987) Integral membrane polypeptides of rat liver peroxisomes: topology and response to different metabolic states. Arch Biochem Biophys 255:109–119PubMedCrossRefGoogle Scholar
  18. 18.
    Hruban Z, Vigil EL, Slesers A, Hopkins E (1972) Microbodies. Constituent organelles of animal cells. Lab Invest 27:184–191PubMedGoogle Scholar
  19. 19.
    Litwin JA, Völkl A, Stachura J, Fahimi HD (1988) Detection of peroxisomes in human liver and kidney fixed with formalin and embedded in paraffin: the use of catalase and lipid β-oxidation enzymes as immunocytochemical markers. Histochem J 20:165–173PubMedCrossRefGoogle Scholar
  20. 20.
    Litwin JA, Yokota S, Hashimoto T, Fahimi HD (1984) Light microscopic immunocytochemical demonstration of peroxisomal enzymes in epon sections. Histochemistry 81:15–22PubMedCrossRefGoogle Scholar
  21. 21.
    Lüers G, Beier K, Hashimoto T, Fahimi HD, Völkl A (1990) Biogenesis of peroxisomes: sequential biosynthesis of the membrane and matrix proteins in the course of hepatic regeneration. Eur J Cell Biol 52:175–184PubMedGoogle Scholar
  22. 22.
    Osmundsen H, Bremer J, Pedersen JI (1991) Metabolic aspects of peroxisomal β-oxidation. Biochim Biophys Acta 1085:141–158PubMedCrossRefGoogle Scholar
  23. 23.
    Reddy JK, Lalwani ND (1983) Carcinogenesis by hepatic peroxisome proliferators: evaluation of the risk of hypolipidemic drugs and industrial plasticizers to humans. CRC Crit Rev Toxicol 12:1–58CrossRefGoogle Scholar
  24. 24.
    Slot JW, Geuze HJ (1985) A new method for preparing gold probes for multiple-labeling cytochemistry. Eur J Cell Biol 38:87–93PubMedGoogle Scholar
  25. 25.
    Völkl A, Baumgart E, Fahimi HD (1988) Localization of urate oxidase in the crystalline cores of rat liver peroxisomes by immunocytochemistry and immunoblotting. J Histochem Cytochem 36:329–336PubMedCrossRefGoogle Scholar
  26. 26.
    Völkl A, Fahimi HD (1985) Isolation and characterization of peroxisomes from the liver of normal untreated rats. Eur J Biochem 149:257–265PubMedCrossRefGoogle Scholar
  27. 27.
    Yamamoto K, Fahimi HD (1987) Biogenesis of peroxisomes in regenerating rat liver. I. Sequential changes of catalase and urate oxidase detected by ultrastructural cytochemistry. Eur J Cell Biol 43:293–300PubMedGoogle Scholar
  28. 28.
    Yamamoto K, Fahimi HD (1987) Three-dimensional reconstruction of a peroxisomal reticulum in regenerating rat liver: evidence of interconnections between heterogeneous segments. J Cell Biol 105:713–722PubMedCrossRefGoogle Scholar
  29. 29.
    Zaar K, Fahimi HD (1991) Immunoelectron microscopic localization of the isozymes of L-α-hydroxyacid oxidase in renal peroxisomes of beef and sheep: evidence of distinct intraorganellar subcompartimentation. J Histochem Cytochem 39:801–808PubMedCrossRefGoogle Scholar
  30. 30.
    Zaar K, Völkl A, Fahimi HD (1991) Purification of marginal plates from bovine renal peroxisomes: identification with L-α-hydroxyacid oxidase B. J Cell Biol 113:113–121PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • E. Baumgart

There are no affiliations available

Personalised recommendations