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Preparation and Purification of Peroxisomes; Subfractionation of Purified Peroxisomes; Acyl-CoA Oxidase Activity Measurements

  • P. P. Van Veldhoven
  • C. Brees
  • A. Völkl
Part of the Springer Laboratory book series (SLM)

Abstract

The isolation of peroxisomes is generally accomplished in three steps. After homogenisation of the tissue or disruption of the cells, a subcellular fraction enriched in peroxisomes is obtained by differential centrifugation (for a comprehensive treatise on centrifugation, see [10]). This fraction is then further separated by means of density gradient centrifugation. Such a procedure will be demonstrated on liver, obtained from control rats and from rats treated with bezafibrate. Hypolipidemic drugs such as bezafibrate cause a pronounced proliferation of peroxisomes in liver and kidney of rodents. This is associated with hepatomegaly and the induction of a particular set of peroxisomal enzymes involved in the β-oxidation of fatty acids [8] (see Exp. 1.3).

Keywords

Peroxisomal Membrane Urate Oxidase Peroxisomal Fraction Bile Acid Intermediate Nycodenz Gradient 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • P. P. Van Veldhoven
  • C. Brees
  • A. Völkl

There are no affiliations available

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