Peroxisomes pp 189-194 | Cite as

In Vitro Transformation of Human Fibroblasts with SV40 T Antigen (Lipofection)

  • K. H. Ng
  • E. B. Bioukar
  • F. Straehli
  • J. Deschatrette
Part of the Springer Laboratory book series (SLM)


Transformed established cell lines are a useful material for molecular genetic investigations of heritable diseases. Indeed, experiments involving the search for correlations between modifications of the genetic material of the cells (fusion, transfection) and phenotypic transitions require long term culture, selection of clones and subclones, and cell characterization, which are difficult to perform with untransformed cells. Furthermore, besides the slow growth rate of untransformed cells, the senescence occuring after a number of cell generations limits the potential investigations performed on usually scarce and unique material. This is especially true for samples obtained from patients suffering from peroxisomal diseases. The still unexplained wide range of clinical and biochemical cases render each sample potentially precious.


Untransformed Cell Plastic Support Molecular Genetic Investigation Peroxisomal Disease Lipofectin Reagent 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Benoist C, Chambon P (1981) In vivo sequence requirements of the SV40 early promoter region. Nature 290:304–310PubMedCrossRefGoogle Scholar
  2. 2.
    Feigner PL, Gadek TR, Holf M, Roman R, Chan HW, Wenz M, Northrop JP, Ringold GM, Danielson M (1987) Lipofection: a novel, highly efficient lipid mediated DNA transfection procedure. Proc Natl Acad Sei USA 84:7413–7417CrossRefGoogle Scholar
  3. 3.
    Girardi AJ, Jensen FC, Koprowski H (1965) SV40-induced transformation of human diploid cells: crisis and recovery. J Cell and Comp Physiol 65:69–84CrossRefGoogle Scholar
  4. 4.
    Huschtscha LI, Holliday R (1983) Limited and unlimited growth of SV40-transformed cells from human diploid MRC-5 fibroblasts. J of Cell Sei 63:77–99Google Scholar
  5. 5.
    Mayne LV, Priestley A, James MR, Burk JF (1986) Efficient immortalization and morphological transformation of human fibroblasts by transfection with SV40 DNA linked to a dominant marker. Exp Cell Res 162:530–538PubMedCrossRefGoogle Scholar
  6. 6.
    Neufeld DS, Ripley S, Henderson A, Ozier HL (1987) Immortalization of human fibroblasts transformed by origin-defective simian virus 40. Mol Cell Biol 7:2794–2802PubMedGoogle Scholar
  7. 7.
    Wright WE, Pereira-Smith OM, Shay JW (1989) Reversible cellular senescence: implications for immortalization of normal human diploid fibroblasts. Mol Cell Biol 9:3088–3092PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • K. H. Ng
  • E. B. Bioukar
  • F. Straehli
  • J. Deschatrette

There are no affiliations available

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