Abstract
It has been shown by various authors that immunization with purines, pyrimidines, nucleosides, mono-, di-, and oligo-nucleotides, or single-stranded polynucleotides (conjugated or complexed to proteins or polypeptides) gives antibodies which are specific for the bases (Butler et al., 1962; Tanenbaum and Beiser, 1963; Karol and Tanenbaum, 1967; Erlanger and Beiser, 1964; Sela et al., 1964; Plescia et al., 1964; Lacour and Harel, 1965; Halloran and Parker, 1966; Seaman et al., 1965). These antibodies react principally with denatured DNA, but not with native DNA, an indication that the immune reaction involves nucleotides that are freely accessible and not involved in a hydrogen-bonded helical secondary structure. Some of these antibodies could also react with RNA.
This work was supported in part by CNRS and INSERM.
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References
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Lacour, F., Michelson, A.M., Nahon, E. (1968). Specific Antibodies to Polynucleotide Complexes and their Reaction with Nucleic Acids: Importance of the Secondary Structure of the Antigen. In: Plescia, O.J., Braun, W. (eds) Nucleic Acids in Immunology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-87668-4_4
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