Summary
Initiation of protein synthesis in tissue culture cells is rapidly inhibited or blocked by addition of either DMSO, ethanol, TPCK, cytochalasin B, or sucrose to the growth medium. In contrast, these agents do not interfere with the initiation of protein synthesis in cell-free extracts to a comparable extent. These results support the hypothesis that protein synthesis in tissue culture cells can be influenced by membrane mediated events. Translation of viral mRNA in RNA virus infected cells is resistant to a number of these inhibitors of peptide chain initiation and proceeds under conditions where translation of host mRNA is almost completely suppressed. It appears that viral mRNA possesses a greater ability than host mRNA to form mRNA-ribosome initiation complexes when the overall rate of peptide chain initiation is reduced. This observation has led to a number of predictions concerning the strategy of virus directed suppression of host mRNA translation. Under optimal growth conditions protein synthesis appears to be regulated mainly, but not exclusively, by the amount of the mRNA available for translation. However, when cellular growth and/or the overall rate of peptide chain initiation is restricted, control of protein synthesis at the translational level becomes decisive with the translation of each mRNA species proceeding with its own characteristic efficiency, most probably as a result of inherent differential affinities of individual mRNA species for ribosomes. Introduction
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Abbreviations
- MEM:
-
Minimal Essential Medium
- HIB:
-
Hypertonic Initiation Block
- VSV:
-
Vesicular Stomatitis Virus
- TPCK:
-
L-1-Tosylamido-2-Phenylethyl Chloromethyl Ketone
- DMSO:
-
Dimethylsulfoxide
- L:
-
Immunoglobulin Light Chain
- H:
-
Immunoglobulin Heavy Chain
- DEAE:
-
Diethylaminoethyl-dextran
- HEPES:
-
N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid
References
Burger, M. M. (1970). Nature 227: 170–171.
Fan, H. and Penman, S. 61970). J. Mol. Biol. 50: 655–670.
Hoffman, A., Bilello, P., Mittelstaedt, R., McFarland, E. and Koch, G. (1975). Arch. Biochem. Biophys. (submitted).
Koch, G. (1973).In, Current Topics in Microbiol. and Immunol. 62: 89–138 ( Braun, Wrand and Wecker ).
Koch, G. (1974). Biochem. Biophys. Res. Commun. 61: 817–824.
Koch, G., Bilello, P., Fishman, M., Mittelstaedt, R. and Borriss, E. (1976). Immune RNA in Neoplasia, Academic Press (in press).
Koch, F. and Koch, G. (1974). Res. Commun. in Chem. Pathol. and Pharma. 9: 291–298.
Koch, G., Kubinski, H. and Koch, F. (1974). Hoppe Seyler’s Z. für Phys. Chemie. 385: 1218.
Koch, G. and Oppermann, H. (1975). Virology 63: 395–403.
Laemmli, U. K. and Favre, M. (1973). J. Mol. Biol. 80: 575–599.
Laskov, R. and Scharff, M. D. (1970). J. Exp. Med. 131: 515–541.
Lawrence, C. and Thach, R. E. (1974). J. Virol. 14: 598–610.
Lodish, H. (1971). J. Biol. Chem. 246: 7131–7138.
Lodish, H. (1974). Nature 251: 385–388.
MacDonald, C. and Gibbs, J. (1969). Biopolymers 7: 707–725.
MacDonald, C., Gibbs, J. and Pipkin, A. (1968). Biopolymers 6: 1–25.
Mans, J. R. and Novelli, G. D. (1961). Arch. Biochem. Biophys. 94: 48–54.
Matthews, T. J., Butterworth, B. E., Chaggin, L. and Rueckert, R. R. (1973). Fed. Proc. 32: 461.
McDowell, M. J., Wolfgang, K. J., Villa-Komaroff, L. and Lodish, H. F. (1972). Proc. Nat. Acad. Sci. USA 69: 2649–2653.
Mudd, J. A. and Summers, D. F. (1970). Virology 42: 328–340.
Nuss, D. L. and Koch, G. (1976a). J. Mol. Biol. (in press).
Nuss, D. L. and Koch, G. (1976b). J. Virol. (in press).
Nuss, D. L. and Koch, G. (1976c). J. Virology (submitted).
Nuss, D. L., Oppermann, H. and Koch, G. (1975). Proc. Nat. Acad. Sci. USA 72: 1258–1262.
Oppermann, H. and Koch, G. (1976a). Arch. of Virol. (in press).
Oppermann, H. and Koch, G. (1976b). J. Gen. Virol. (in press).
Oppermann, H., Saborio, J. L., Zarucki, T. and Koch, G. (1973). Fed. Proc. Fed. Amer. Soc. Exp. Biol. 32: 53.
Pong, S.-S., Nuss, D. L. and Koch, G. (1975).. J. Biol. Chem. 250: 240–245.
Racevskis, J., Kerwar, S. and Koch, G. (1976). J. Gen. Virol. (in press).
Saborio, J. L. and Koch, G. (1973). J. Biol. Chem. 248: 8343–8347.
Saborio, J. L., Pong, S.-S. and Koch, G. (1975). J. Mol. Biol. 85: 195–211.
Saborio, J. L., Wiegers, K. J. and Koch, G. (1975). Arch. Virol. 49: 81–87.
Sefton, B. M. and Rubin, H. (1970). Nature 227: 843–845.
Srere, P. A. (1974). In, Seventh Annual Miami Winter Symposia on “Biology and Chemistry of Eucaryotic Cell Surfaces”, 7: 21–47 ( Lee, E. Y. C. and Smith, E. E., ).
Wright, P. J. and Cooper, P. D. (1974). Virology 59: 1–20.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1976 J. F. Lehmanns Verlag München
About this paper
Cite this paper
Koch, G., Oppermann, H., Bilello, P., Koch, F., Nuss, D. (1976). Control of Peptide Chain Initiation in Uninfected and Virus Infected Cells by Membrane Mediated Events. In: Neth, R., Gallo, R.C., Mannweiler, K., Moloney, W.C. (eds) Modern Trends in Human Leukemia II. Hämatologie und Bluttransfusion, vol 19. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-87524-3_51
Download citation
DOI: https://doi.org/10.1007/978-3-642-87524-3_51
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-79785-2
Online ISBN: 978-3-642-87524-3
eBook Packages: Springer Book Archive