Sequences and Functions of Rous Sarcoma Virus RNA
A procedure has been developed to map the genetic elements of avian tumor virus RNA, which has a molecular weight of about 3 × 106 daltons and a poly(A) sequence at the 3’ end. For this purpose, about 30 RNase T1-resistant oli gonucleotides were ordered relative to the 3’-poly(A) terminus of the RNA, to construct an oligonucleotide map of viral RNAs. A cluster of seven envelope gene (env)-specific oligonucleotides, identified by their absence from the otherwise very similar oligonucleotide map of an envelope-defective deletion mutant (which lacks the major viral glycoprotein), mapped at a distance of 0.9 to 1.6 × 106 daltons from the poly(A) end of sarcoma virus RNA. A cluster of three sarcoma gene (src)-specific oligonucleotides, identified by their absence from the otherwise nearly identical oligonucleotide map of a transformation-defective deletion mutant mapped at a distance of 0.2 to 0.6 × 106 daltons from the poly(A) end of sarcoma virus RNA. The oligonucleotide maps of sarcoma viruses and of related deletion mutants were the same from the poly(A) end up to 0.2 × 106 daltons and included one terminal oligonucleotide, termed C, which is found in all avian tumor viruses tested so far. Preliminary mapping experiments ordering the src-specific and env-specific oligonucleotides of recombinants, selected for sarcoma and envelope genes of different parents, agree with those obtained by comparing maps of wild type viruses and deletion mutants. A partial genetic map consistent with these results suggests that the src gene maps between the env gene and the 3’-poly(A) end of viral RNA. This map reads: poly(A)-src-env-(pol, gag).
KeywordsDeletion Mutant Fingerprint Analysis Fingerprint Pattern Sucrose Gradient Fractionation Exhaustive Digestion
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- 1.Duesberg, P. H. (1970 “Current Topic Microbiol. and Immunology” 51, 79–104.Google Scholar
- 2.Tooze, J. (1973) in The Molecular Biology of Tumour Vuruses. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, N.Y.Google Scholar
- 11.Barrell, G. G. (1971) in Procedures in Nucleic Acid Research (eds. S. L. Cantoni and D. R. Davis) Vol. 2, Harper and Row, New York, N.Y.Google Scholar
- 14.Kawai, S., and Hanafusa, H. (1973) Proc. Nat. Acad. Sci. USA 70, 3493–3497.Google Scholar
- 18.Wang, L. H., Duesberg, P. H., Mellon, P. and Vogt, P. K. (1976) Proc. Nat. Acad. Sci., USA 73, 1073–1077.Google Scholar
- 19.Duesberg, P. H., Wang L. H., Mellon, P., Mason W. S. and Vogt, P. K. (1976) in: Proceedings of the ICN-UCLA Symposium (1976) on Animal Virology, eds. Baltimore, D., Huang, A. and Fox, F. (Academic Press, New York), in press.Google Scholar