Summary
In most systems involving cellular differentiation and cellular transformation the biological process is non-synchronous and the sample heterogeneous. In order to answer some of the basic questions about the control mechanisms of cellular changes and the order in which they proceed one must have access to homogeneous classes of cells. Friend virus transformed erythroid cells which are stably maintained in tissue culture can be chemically induced to differentiate and are thus very advantageous for in vitro studies (1–3).
With such a system the questions which we pose are a) the reversibility of the differentiation process; b) the order of steps in the production of specialized messenger RNA; c) the time of shut-off of undifferentiated messenger production; d) the relationship of viral RNA production to the differentiation process; e) the onset and extent of specific protein synthesis; f) the correlation of DNA metabolism with the timing or course of events. By using a computer-controlled cell separator we can select live cells on the basis of their macromolecular content, membrane properties (using a new parameter, fluorescence emission anisotropy), and size (4, 5, 34). Thus with proper probes as described here, we are able to select cells at different stages in their differentiation and can begin to attack the questions posed above.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Friend, C., Patuleia, M. C., and De Harven, E., National Cancer Institute Monograph 22: 505, 1966.
Friend, C., Scher, W., Holland, J. G., and Sato, T., Proc. Nat. Acad. Sci. USA 68: 378, 1971.
Ostertag, W., Melderis, H., Steinheider, G., Kluge, N., and Dube, S. K., Nature New Biol. 239: 231, 1972.
Arndt-Jovin, D., and Jovin, T. M., J. Histochem. Cytochem. 22:, 622, 1974.
Arndt-Jovin, D., Ostertag, W., Eisen, H., Klimek, F., and Jovin, T. M., J. Histochem. Cytochem. 24: 332, 1976.
Bonner, W. A., Hulett, H. R., Sweet, R. G., and Herzenberg, L. A., Rev. Sci. Instrum. 43: 404, 1972.
Steinkamp, J. A., Fulwyler, M. J., Coulter, J. R., Hiebert, R. D., Horney, J. L., and Mullaney, P. F., Rev. Sci. Instrum. 44: 1301, 1973.
Dube, S. K., Gaedicke, G., Kluge, N., Weimann, B. J., Melderis, H., Steinheider, G., Crozier, T., Beckmann, H., and Ostertag, W., in Differentiation and Control of Malignancy of Tumor Cells. (Eds. W. Nakahara, T. Ono, T. Sugimura, H. Sugano ), University of Tokyo Press, Tokyo, 1974, p. 99.
Gaedicke, G., Abedin, Z., Dube, S. K., Kluge, N., Neth, R., Steinheider, G., Weimann, B. J., Ostertag, W., in Modern Trends in Human Leukemia (Eds. R. Neth, R. Gallo, S. Spiegelman, F. Stohlman ), Grune and Stratton, New York, 1974, p. 278–287.
Ostertag, W., Cole, T., Crozier, T., Gaedicke, G., Kind, J., Kluge, N., Krieg, J. C., Roesler, G., Steinheider, G., Weimann, B. J., and Dube, S. K., in Differentiation and Control of Malignancy of Tumor Cells. (Eds. W. Nakahara, T. Ono, T. Sugimura, H. Sugano ), University of Tokyo Press, Tokyo, 1974, p. 485.
Tanaka, M., Levy, J., Terada, M., Breslow, R., Rifkind, R. A., and Marks, P. A., Proc. Nat. Acad. Sci. USA 72: 1003, 1975.
Leder, A., and Leder, P., Cell 5: 319, 1975.
Takahashi, E., Yamada, M., Saito, M., Kuboyama, M., and Ogasa, K., Gann, 66:, 577, 1975.
Gilmour, R. S., Harrison, P. R., Windass, J. D., Affara, N. A., and Paul, J., Cell Differentiation 3: 9, 1974.
Harrison, P. R., Gilmour, R. S., Affara, N. A., Conkie, D., and Paul, J., Cell Differentiation 3: 23, 1974.
Ross, J., Ikawa, Y., Leder, P., Proc. Nat. Acad. Sci. USA 69: 3620, 1972.
Pragnell, I. B., Ostertag, W., Steinheider, G., Takahashi, E., Paul, J., Williamson, R., submitted for publication.
Dube, S. K., Pragnell, I. B., Kluge, N., Gaedicke, G., Steinheider, G., and Ostertag, W., Proc. Nat. Acad. Sci. USA 72: 1863, 1975.
Ross, J., Gielen, J., Packman, S., Ikawa, Y., and Leder, P., J. Mol. Biol. 87: 697, 1974.
Orkin, S. H., Harosi, F. I., Leder, P., Proc. Nat. Acad. Sci. USA 72: 98, 1975.
Eisen, H., Ostertag, W., and Arndt-Jovin, D. J., submitted for publication.
Jovin, T. M., Morris, S. J., Striker, G., Schultens, H., Digweed, M., and ArndtJovin, D., J. Histochem. Cytochem. 24: 269, 1976.
Arndt-Jovin, D., Jovin, T. M., FEBS Letters 44: 247, 1974.
Klein, J., Biology of the Mouse Histocompatibility-2 Complex. Springer, Heidelberg, 1975, p. 620.
Van Deenen, L. L. M., and De Gier, J., in The Red Blood Cell (ed. D. M. N. Surgenor), Vol 1, Academic Press, New York and London, 1974, p. 147.
Perrin, F., J. Phys. Radium 7: 390, 1926.
Jablonski, A., Acta Phys. Polon. 16: 471, 1957.
Jablonski, A., Bull. Acad. Polon. Sci., Sér sci. math astr. phys. 8: 259, 1960.
Shinitzky, M., and Inbar, M., J. Mol. Biol. 85: 603, 1974.
Shinitzky, M., and Inbar, M., (in press).
Steward, F. C., Mapes, M. O., Kent, A. E., and Holsten, R. D., Science 143: 20, 1964.
Inbar, M., and Shinitzky, M., Proc. Nat. Acad. Sci. USA 71: 2128, 1974.
Inbar, M., and Shinitzky, M., Proc. Nat. Acad. Sci. USA 71: 4229, 1974.
Arndt-Jovin, D. J., and Jovin, T. M., in Membranes and Neoplasia, Progress in Clinical and Biological Research. (Ed. F. Fox), Alan Liss, Inc., New York,1976, in press.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1976 J. F. Lehmanns Verlag München
About this paper
Cite this paper
Arndt-Jovin, D.J., Ostertag, W., Eisen, H., Jovin, T.M. (1976). Analysis by Computer-controlled Cell Sorter of Friend Virus-transformed Cells in Different Stages of Differentiation. In: Neth, R., Gallo, R.C., Mannweiler, K., Moloney, W.C. (eds) Modern Trends in Human Leukemia II. Hämatologie und Bluttransfusion, vol 19. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-87524-3_14
Download citation
DOI: https://doi.org/10.1007/978-3-642-87524-3_14
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-79785-2
Online ISBN: 978-3-642-87524-3
eBook Packages: Springer Book Archive