Abstract
Metaphase chromosome preparations from primary cultures are usually of better quality than those from bone marrow. However, in a number of cases bone marrow is the only practical material for cytological preparations; therefore, mastering this technique is essential in studies of mammalian karyotypes. These reasons include:
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1
Not every investigator has a tissue culture laboratory at his disposal.
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2
Direct bone marrow preparations reduce the cost.
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3
In field trips, bone marrows can be processed with relative ease without large pieces of equipment and supply items, such as fresh growth media.
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4
Bone marrow preparations should be made as an insurance in case primary cultures fail or become contaminated.
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5
In case of mosaicism, bone marrow offers an excellent second material.
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© 1969 Springer-Verlag New York Inc.
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Hsu, T.C., Patton, J.L. (1969). Bone Marrow Preparations for Chromosome Studies. In: Benirschke, K. (eds) Comparative Mammalian Cytogenetics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-85943-4_28
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DOI: https://doi.org/10.1007/978-3-642-85943-4_28
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-85945-8
Online ISBN: 978-3-642-85943-4
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