Consecutive Determination of the Levels of Twelve Anti-Epileptic Drugs in Blood and Cerebrospinal Fluid
In Japan, as in other countries, we administer a combination of anti-epileptic drugs in refractory cases. At the last workshop we reported a method for the consecutive determination of five drugs by gas chromatography (Miyamoto et al., 1973). Since then, the number of drugs that can be determined from the same specimen has increased to eight: phenytoin (DPH), ethotoin, phenobarbitone (PB), methyl phenobarbitone, primidone, carbamazepine, pheneturide and acetyl pheneturide. A minor modification of the earlier procedure enabled us to obtain better chromatograms and to minimize peaks caused by contaminants. In addition, by applying Meijer’s extraction procedure (Meijer, 1973) for anti-petit mal drugs, we are analysing metharbitone and benzodiazepines in addition to trimethadione (TMO), dimethadione (DMO) and ethosuximide.
KeywordsControl Serum Extraction Centrifuge Good Recovery Rate Consecutive Determination Coiled Column
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